UL122 (NC_006273) Virus Tagged ORF Clone

CAT#: VC101293

Myc-DDK-tagged ORF clone of viral ORF for regulatory protein IE2 [Human herpesvirus 5], codon optimized for human cell expression, YP_081561

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UL122
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101293 represents NCBI reference of YP_081561 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGCAGCGCCAAACGGAAAATGGATCCTGACAATCCCGATGAGGGACCTAGCAGCAAAGTGCCAC
  GGCCCGAGACCCCAGTGACCAAGGCCACCACATTCCTGCAGACTATGCTGCGGAAGGAGGTCAACAGCCA
  ACTGTCCCTGGGCGACCCCCTCTTCCCCGAGCTCGCCGAAGAGAGCCTGAAAACCTTCGAGCAGGTCACC
  GAGGACTGCAACGAGAACCCCGAAAAGGACGTGCTGACCGAGCTCGGCGACATCCTCGCACAGGCCGTGA
  ACCACGCCGGCATCGACAGCAGCAGCACCGGCCCCACACTGACCACCCACAGCTGCAGCGTGTCCAGCGC
  CCCCCTGAACAAGCCCACTCCCACCTCCGTGGCCGTCACCAATACCCCCCTGCCCGGCGCCTCCGCCACC
  CCCGAGCTGAGCCCCCGGAAGAAGCCCCGGAAAACCACACGGCCCTTCAAGGTCATCATCAAGCCCCCAG
  TGCCACCCGCCCCCATCATGCTCCCCCTGATTAAGCAGGAGGACATTAAGCCCGAGCCCGACTTCACCAT
  CCAGTACCGGAACAAGATCATCGACACTGCCGGCTGCATCGTGATCTCCGACTCCGAGGAGGAGCAGGGC
  GAGGAGGTCGAGACCCGGGGAGCCACCGCCAGCAGCCCCTCCACCGGCAGCGGCACACCCCGGGTGACCA
  GCCCCACCCACCCTCTGAGCCAGATGAACCACCCCCCCCTCCCCGACCCCCTCGGGCGGCCCGATGAGGA
  CAGCAGCAGCAGCAGCTCCAGCAGCTGCAGCAGCGCCAGCGACAGCGAGTCCGAAAGCGAGGAGATGAAG
  TGCAGCAGCGGGGGCGGCGCAAGCGTGACAAGCTCCCACCACGGCCGGGGCGGGTTCGGGGGCGCCGCCA
  GCTCCTCCCTGCTGAGCTGCGGCCATCAGAGCTCCGGCGGCGCCAGCACTGGCCCCCGGAAAAAGAAGAG
  CAAGCGGATCAGCGAGCTCGACAATGAGAAGGTGCGGAACATCATGAAGGACAAGAATACCCCCTTCTGC
  ACCCCAAACGTGCAAACCAGACGGGGCCGGGTGAAGATCGACGAGGTGAGCCGGATGTTCCGGCACACCA
  ACCGGTCCCTGGAATACAAGAATCTGCCCTTCATGATCCCCAGCATGCACCAGGTGCTCGAGGAGGCCAT
  CAAGGTCTGCAAGACCATGCAGGTGAACAACAAGGGCATCCAGATCATTTACACCCGGAACCACGAAGTG
  AAGAACGAGGTCGACCAGGTCAGATGCCGGCTCGGCAGCATGTGCAACCTGGCCCTGAGCACCCCCTTCC
  TCATGGAGCACACCATGCCCGTCACCCACCCCCCCGACGTGGCACAACGGACCGCCGACGCCTGCAACGA
  CGGCGTCAAGGCCGTGTGGAACCTCAAGGAGCTCCATACCCACCAGCTGTGCCCCCGGAGCAGCGACTAC
  CGGAACATGATCATCCACGCCGCCACACCCGTGGACCTGCTGGGCGCCCTGAACCTCTGCCTCCCCCTGA
  TGCAGAAGTTCCCAAAGCAGGTGATGGTGCGGATCTTCAGCACCAACCAAGGGGGATTCATGCTCCCTAT
  TTACGAGACCGCCGCCAAAGCCTACGCTGTGGGCCAATTCGAAAAGCCCACCGAAACCCCCCCCGAAGAC
  CTGGACACTCTGTCCCTGGCCATCGAGGCCGCCATCCAGGATCTCCGGAATAAGAGCCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101293 representing YP_081561
  Red=Cloning sites Green=Tags
  
  MESSAKRKMDPDNPDEGPSSKVPRPETPVTKATTFLQTMLRKEVNSQLSLGDPLFPELAEESLKTFEQVT
  EDCNENPEKDVLTELGDILAQAVNHAGIDSSSTGPTLTTHSCSVSSAPLNKPTPTSVAVTNTPLPGASAT
  PELSPRKKPRKTTRPFKVIIKPPVPPAPIMLPLIKQEDIKPEPDFTIQYRNKIIDTAGCIVISDSEEEQG
  EEVETRGATASSPSTGSGTPRVTSPTHPLSQMNHPPLPDPLGRPDEDSSSSSSSSCSSASDSESESEEMK
  CSSGGGASVTSSHHGRGGFGGAASSSLLSCGHQSSGGASTGPRKKKSKRISELDNEKVRNIMKDKNTPFC
  TPNVQTRRGRVKIDEVSRMFRHTNRSLEYKNLPFMIPSMHQVLEEAIKVCKTMQVNNKGIQIIYTRNHEV
  KNEVDQVRCRLGSMCNLALSTPFLMEHTMPVTHPPDVAQRTADACNDGVKAVWNLKELHTHQLCPRSSDY
  RNMIIHAATPVDLLGALNLCLPLMQKFPKQVMVRIFSTNQGGFMLPIYETAAKAYAVGQFEKPTETPPED
  LDTLSLAIEAAIQDLRNKSQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006273
• ORF Size: 1740 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006273.2, YP_081561
• RefSeq ORF: 1740
• Locus ID: 3077563
• MW: 63.1 kDa