UL55 (NC_006273) Virus Tagged ORF Clone

CAT#: VC101245

Myc-DDK-tagged ORF clone of viral ORF for envelope glycoprotein B [Human herpesvirus 5], codon optimized for human cell expression, YP_081514

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UL55
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101245 represents NCBI reference of YP_081514 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTCTCGCATCTGGTGTCTCGTCGTTTGTGTAAACTTGTGTATCGTTTGTCTCGGTGCTGCCGTAT
  CCAGCTCTAGTACGAGGGGAACCTCCGCAACTCACTCCCACCACAGCTCACACACCACAAGTGCGGCACA
  TTCAAGGTCCGGGAGTGTGTCTCAGAGAGTGACATCCAGCCAGACTGTTTCACATGGAGTAAACGAGACT
  ATATATAACACTACCCTTAAATACGGCGACGTTGTGGGCGTGAACACTACTAAGTACCCTTACAGAGTGT
  GTTCCATGGCCCAGGGAACAGACCTGATCCGGTTCGAGCGGAACATCGTGTGTACTAGCATGAAACCAAT
  CAACGAGGATCTCGATGAGGGAATTATGGTTGTGTACAAACGCAACATCGTTGCACATACTTTTAAGGTA
  AGAGTGTATCAGAAAGTGCTGACTTTTAGGCGGTCCTATGCCTACATCCACACCACTTACCTGCTGGGCT
  CAAATACAGAGTATGTGGCACCTCCAATGTGGGAAATTCACCACATCAATTCTCATAGCCAGTGCTATAG
  CAGCTATTCTCGCGTGATAGCCGGAACCGTGTTCGTTGCATATCATAGAGATTCCTATGAGAACAAAACC
  ATGCAGCTCATGCCCGACGATTACTCCAACACTCACAGTACAAGGTATGTCACCGTCAAGGATCAGTGGC
  ACAGTCGAGGAAGCACGTGGCTCTACAGAGAGACATGCAATCTGAACTGTATGGTTACCATAACTACGGC
  CAGATCAAAATATCCTTATCATTTTTTCGCGACTTCCACCGGCGATGTGGTGGACATTAGCCCTTTCTAC
  AACGGCACTAACAGAAATGCGAGCTACTTTGGGGAGAATGCCGATAAATTTTTTATCTTCCCAAATTACA
  CCATAGTGTCAGATTTCGGCAGACCTAACTCAGCCCTGGAAACCCACCGATTGGTCGCCTTCCTGGAGCG
  CGCAGATTCCGTGATCAGCTGGGATATTCAAGACGAAAAGAACGTTACCTGCCAGCTGACATTCTGGGAG
  GCCTCCGAGAGAACTATCAGAAGTGAAGCCGAGGATAGCTACCATTTTAGCAGTGCGAAGATGACGGCCA
  CGTTCCTTTCAAAGAAACAAGAGGTGAACATGAGCGACTCCGCCTTGGACTGCGTTAGGGACGAGGCGAT
  CAACAAGCTTCAGCAGATTTTCAATACATCTTACAACCAGACCTACGAGAAGTACGGAAATGTGTCCGTA
  TTCGAGACAACGGGAGGGCTGGTGGTATTTTGGCAGGGTATTAAACAGAAAAGCCTCGTGGAGCTCGAAC
  GGCTGGCTAACAGGAGCTCTCTGAATCTTACCCACAATCGGACGAAACGGTCCACTGACGGCAACAACGC
  TACACATTTGAGTAACATGGAAAGCGTGCACAATCTTGTGTACGCCCAGCTCCAGTTCACTTACGATACC
  CTGCGCGGGTACATCAACAGGGCACTGGCCCAGATCGCCGAGGCATGGTGTGTTGATCAGAGGCGCACTT
  TGGAGGTCTTCAAGGAGCTCTCCAAGATCAACCCCTCTGCCATTCTTAGCGCTATCTACAACAAGCCCAT
  TGCTGCCAGGTTCATGGGGGATGTCCTGGGCCTGGCTTCCTGCGTCACCATTAATCAGACGAGTGTTAAG
  GTGCTCCGAGACATGAATGTGAAGGAGAGTCCCGGGAGATGCTACTCCCGACCCGTCGTGATTTTCAACT
  TCGCGAACAGCAGCTATGTGCAGTACGGCCAACTGGGAGAAGATAACGAAATCCTCCTGGGTAACCACCG
  GACCGAAGAATGTCAGTTGCCTAGCCTGAAGATTTTCATTGCTGGCAACTCCGCTTATGAGTATGTGGAC
  TATTTGTTCAAGAGGATGATTGACCTCAGTAGCATCTCAACAGTGGACAGTATGATCGCTTTGGATATAG
  ACCCACTGGAGAACACAGACTTTAGAGTCCTGGAACTGTACTCCCAGAAAGAGCTCAGATCTTCCAACGT
  GTTCGATTTGGAGGAGATAATGCGCGAGTTTAATTCTTACAAACAACGGGTGAAGTACGTGGAAGACAAG
  GTGGTGGATCCCCTCCCCCCCTATCTCAAAGGACTGGATGATCTTATGAGTGGTCTGGGAGCCGCAGGTA
  AGGCTGTGGGAGTGGCAATCGGTGCCGTTGGGGGGGCCGTAGCCAGTGTCGTCGAAGGTGTGGCGACTTT
  CCTGAAGAACCCCTTCGGCGCCTTCACGATCATCCTCGTGGCCATCGCTGTAGTCATTATCACTTACTTG
  ATTTATACTAGACAACGGAGACTCTGCACGCAGCCTCTGCAGAATCTGTTCCCTTACCTTGTATCTGCCG
  ATGGAACAACTGTCACCAGCGGCTCAACCAAGGATACGAGCTTGCAGGCTCCTCCGAGTTATGAAGAATC
  TGTTTACAACAGCGGCCGGAAGGGACCAGGCCCACCCAGTAGCGACGCCAGCACTGCCGCCCCACCATAC
  ACTAACGAACAGGCCTACCAGATGCTGCTTGCTTTGGCTAGGCTGGACGCTGAACAGCGCGCACAACAAA
  ACGGGACAGACAGTCTGGACGGGCGGACCGGAACCCAGGATAAGGGGCAGAAGCCCAACTTGCTTGATCG
  GCTGCGGCACCGGAAGAACGGCTATCGGCATCTCAAAGATAGCGACGAAGAGGAGAACGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101245 representing YP_081514
  Red=Cloning sites Green=Tags
  
  MESRIWCLVVCVNLCIVCLGAAVSSSSTRGTSATHSHHSSHTTSAAHSRSGSVSQRVTSSQTVSHGVNET
  IYNTTLKYGDVVGVNTTKYPYRVCSMAQGTDLIRFERNIVCTSMKPINEDLDEGIMVVYKRNIVAHTFKV
  RVYQKVLTFRRSYAYIHTTYLLGSNTEYVAPPMWEIHHINSHSQCYSSYSRVIAGTVFVAYHRDSYENKT
  MQLMPDDYSNTHSTRYVTVKDQWHSRGSTWLYRETCNLNCMVTITTARSKYPYHFFATSTGDVVDISPFY
  NGTNRNASYFGENADKFFIFPNYTIVSDFGRPNSALETHRLVAFLERADSVISWDIQDEKNVTCQLTFWE
  ASERTIRSEAEDSYHFSSAKMTATFLSKKQEVNMSDSALDCVRDEAINKLQQIFNTSYNQTYEKYGNVSV
  FETTGGLVVFWQGIKQKSLVELERLANRSSLNLTHNRTKRSTDGNNATHLSNMESVHNLVYAQLQFTYDT
  LRGYINRALAQIAEAWCVDQRRTLEVFKELSKINPSAILSAIYNKPIAARFMGDVLGLASCVTINQTSVK
  VLRDMNVKESPGRCYSRPVVIFNFANSSYVQYGQLGEDNEILLGNHRTEECQLPSLKIFIAGNSAYEYVD
  YLFKRMIDLSSISTVDSMIALDIDPLENTDFRVLELYSQKELRSSNVFDLEEIMREFNSYKQRVKYVEDK
  VVDPLPPYLKGLDDLMSGLGAAGKAVGVAIGAVGGAVASVVEGVATFLKNPFGAFTIILVAIAVVIITYL
  IYTRQRRLCTQPLQNLFPYLVSADGTTVTSGSTKDTSLQAPPSYEESVYNSGRKGPGPPSSDASTAAPPY
  TNEQAYQMLLALARLDAEQRAQQNGTDSLDGRTGTQDKGQKPNLLDRLRHRKNGYRHLKDSDEEENV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006273
• ORF Size: 2721 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006273.2, YP_081514
• RefSeq ORF: 2721
• Locus ID: 3077424
• MW: 101.9 kDa