RL13 (NC_006273) Virus Tagged ORF Clone

CAT#: VC101193

Myc-DDK-tagged ORF clone of viral ORF for membrane protein RL13 [Human herpesvirus 5], codon optimized for human cell expression, YP_081461

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RL13
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101193 represents NCBI reference of YP_081461 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATTGGCATCTGGCCATCACCTGGACAGTTATCATACTCACCTTCAGCGAGTGCTATAACCAGACCT
  GCCCTTGCCCTTGTATTTGCGTGAATAGTACGACAGTGTCTATTTCTACATCTGAGACAACGTCCAAGAA
  CATCACCCCTACGACTACAACAAACTCCAAAAAAACAACCAGTAGCATCGCAACCACAACGCCTTCCTTG
  GTAACTACAGGCAAAGTGGTGTCTACCGCTGCCTCCTCTACCATTATAAGCCAGACCAACCGCAGCCATA
  CCTCCAACGCCATTACGACACCCAAGACTCGATTTGAGTACAATATCACGGGGTACGTGGGACAGGAGGT
  GACCTTGAATTTCACCGGATCTTGGAACTATATCCAGTGGTTTCGGTATGGTAGCCCAGGGTGGCTGTAC
  AGTTCTGAGCCCATTTGCACCGTCACTAGTAATTACCATCACACTTTTCCCAGGGGCGCTCTTTGTTTTG
  ATTGCGATATGACCAAGCTGCTGATATATGACCTCACACTCAACGACAGTGGAAAATACGTGGTCAAAAG
  GACACGCCATGATAACCAGTACGAGGAGGCTTGTTATTCACTGACCGTGATATTCGCAAATACTACAAGC
  ATCGTCACGAACCGAACATGTGATCGCAAAAGGACGGAAAACACAGACACAACCAACCACGAGATTGGCA
  AGCACATAATAGAAACCATCAAAAAGGCTAATATCCCTTTGGGCATTCATGCTGTGTGGGCCGGCATCGT
  TGTAAGCGTGGCCTTGATCGCTCTCTATATGGGGAACAGGCGGCGCCCCAGAAAGCCTAGATATACTCGC
  CTGCCCAAGTACGACCCCGATGAAAGCTGGACAAAAACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101193 representing YP_081461
  Red=Cloning sites Green=Tags
  
  MHWHLAITWTVIILTFSECYNQTCPCPCICVNSTTVSISTSETTSKNITPTTTTNSKKTTSSIATTTPSL
  VTTGKVVSTAASSTIISQTNRSHTSNAITTPKTRFEYNITGYVGQEVTLNFTGSWNYIQWFRYGSPGWLY
  SSEPICTVTSNYHHTFPRGALCFDCDMTKLLIYDLTLNDSGKYVVKRTRHDNQYEEACYSLTVIFANTTS
  IVTNRTCDRKRTENTDTTNHEIGKHIIETIKKANIPLGIHAVWAGIVVSVALIALYMGNRRRPRKPRYTR
  LPKYDPDESWTKT
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006273
• ORF Size: 879 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006273.2, YP_081461
• RefSeq ORF: 879
• Locus ID: 3077492
• MW: 33.0 kDa