UL100 (NC_006273) Virus Tagged ORF Clone

CAT#: VC101279

Myc-DDK-tagged ORF clone of viral ORF for envelope glycoprotein M [Human herpesvirus 5], codon optimized for human cell expression, YP_081547

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UL100
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101279 represents NCBI reference of YP_081547 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCCAGCCACGTGGACAAGGTGAACACTCGGACCTGGTCCGCAAGCATCGTGTTTATGGTGCTCA
  CCTTCGTGAACGTGAGCGTCCACCTGGTGCTGAGCAACTTCCCCCACCTGGGCTACCCCTGCGTGTACTA
  CCACGTGGTGGACTTCGAGCGGCTCAACATGTCCGCCTACAACGTGATGCACCTGCACACCCCCATGCTC
  TTCCTCGACAGCGTGCAGCTCGTGTGCTACGCCGTGTTCATGCAGCTCGTGTTCCTGGCCGTGACCATCT
  ACTACCTGGTCTGTTGGATCAAGATTAGCATGCGGAAGGACAAGGGAATGTCCCTCAACCAGAGCACCCG
  GGACATCTCCTACATGGGGGACTCCCTGACCGCCTTCCTGTTCATCCTGAGCATGGACACCTTCCAGCTG
  TTCACCCTGACCATGAGCTTCCGGCTGCCCAGCATGATCGCCTTCATGGCCGCCGTGCACTTCTTCTGCC
  TGACCATTTTCAACGTGTCCATGGTGACCCAGTATCGGAGCTACAAGAGAAGCCTCTTCTTCTTCAGCCG
  GCTCCATCCCAAGCTCAAGGGCACCGTGCAGTTCCGGACCCTCATCGTGAACCTGGTCGAGGTGGCCCTG
  GGGTTCAACACCACAGTCGTGGCTATGGCACTGTGCTACGGGTTTGGGAACAACTTTTTTGTGCGGACCG
  GCCACATGGTGCTGGCCGTGTTCGTGGTGTACGCCATCATCTCCATCATCTACTTCCTGCTGATCGAGGC
  CGTCTTTTTCCAGTACGTGAAGGTGCAGTTCGGCTACCATCTCGGCGCCTTCTTCGGCCTCTGCGGCCTG
  ATTTACCCCATCGTGCAGTACGACACCTTCCTCAGCAACGAGTACCGGACCGGCATCAGCTGGAGCTTCG
  GCATGCTGTTCTTCATCTGGGCCATGTTCACCACCTGCCGGGCCGTGCGGTACTTTCGGGGACGGGGCTC
  CGGCTCCGTGAAGTACCAGGCCCTCGCCACCGCATCCGGCGAGGAGGTCGCCGCCCTGTCCCACCACGAC
  TCCCTGGAGAGCAGACGGCTGCGGGAGGAAGAGGACGACGACGACGAGGACTTTGAGGATGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101279 representing YP_081547
  Red=Cloning sites Green=Tags
  
  MAPSHVDKVNTRTWSASIVFMVLTFVNVSVHLVLSNFPHLGYPCVYYHVVDFERLNMSAYNVMHLHTPML
  FLDSVQLVCYAVFMQLVFLAVTIYYLVCWIKISMRKDKGMSLNQSTRDISYMGDSLTAFLFILSMDTFQL
  FTLTMSFRLPSMIAFMAAVHFFCLTIFNVSMVTQYRSYKRSLFFFSRLHPKLKGTVQFRTLIVNLVEVAL
  GFNTTVVAMALCYGFGNNFFVRTGHMVLAVFVVYAIISIIYFLLIEAVFFQYVKVQFGYHLGAFFGLCGL
  IYPIVQYDTFLSNEYRTGISWSFGMLFFIWAMFTTCRAVRYFRGRGSGSVKYQALATASGEEVAALSHHD
  SLESRRLREEEDDDDEDFEDA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006273
• ORF Size: 1113 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006273.2, YP_081547
• RefSeq ORF: 1113
• Locus ID: 3077534
• MW: 42.7 kDa