US22 (NC_006273) Virus Tagged ORF Clone

CAT#: VC101339

Myc-DDK-tagged ORF clone of viral ORF for tegument protein US22 [Human herpesvirus 5], codon optimized for human cell expression, YP_081607

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: US22
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101339 represents NCBI reference of YP_081607 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCTGCTGACTAAGGCCGCAGCCGAAGCCTGGGGCACCTATCTGCGGCAGAGAGATGAGCGGTGCG
  AAGACGCCATTCGGTGCGATTACGGCGTGTTCCAATTTAGAAACACCGTGTTTCAGAAGACACTGAGCAT
  GCTGCAGGGCCTGTATCTCCGGCAATACGATCCCCCCGCCCTCAGAACCTACGTCCAACGGCATCAGGGG
  ACTACCGTGGCACTGCGGAACCCCGCCAACTGGTTCCTCGTCATGCGGGAGCAGGCCGCCATCCCTCAGA
  TCTACGCCAGAAGCCTCGCCGCAGATTACCTGTGTTGCGACGACACCCTGGAAGCCGTCGGCGTGCTGGC
  CGTCCGGCCACCCGACAGCGACCTGACCCGGAACACAAAGCAGGCCCAGGAGCTCCCATGCGTCCTCATG
  CTCAGCCACTACGGGACCGTGTACGTCTACGACTGGGAGACCGACGGGCTGTACGAAGTGGCAAGCGACA
  TCAAGGCCTTCAGCAAGAACGGCCTGCTCTGGTGCGAGTACGTGTACCGGCACCCCCAGACACCCTTCGC
  CACCACCGAGCCCCGGTATCACGTGCAGAAATTCCTGTGCACCGACCCCACCGATGCTGCCGCCGTGGCC
  AAGACCGCCCGGGAGATGAACGGCCTGAACCTGGTGATTCGGACCCCCGGGCGGACCGAGGTCGAGCCCC
  TCCTCATGCTCGGCAGCATTGAAGGCCTGCGGGCCTGCCGGCCCTTCGACCACATGCCCGCTGCCGACTT
  CCGGGACCTGCTCAACTTCATCCGGCAGCGGCTGTGCTGTGAGTGGTACGTCGTGGGCCTGGTCGGCTAC
  TACCTCGCCTACGGCCCCTTTGTCCCCTCCGGCATGGTCCTGCTCGATAAGTTCGGCGTGGTGTACCTGC
  ACAAGATCGAGGACTCCGACCTGTATCGGATCGCCGACAACTTCCACATGTTCCTGAAGTGCGGCCTCCT
  CAAACTGCGGGGCCTGTGCCGGTTCGACCGGGGGCTGCGGGGCGAATGCCGGCTGGAAGAACTGCCCGTG
  TGCCACCACACCCTGAAGCGGGACGTGCTGCGGTGGCATGGCGCCCTGGGCACCATCACCCGGAGCCAAC
  TCGAGTCCGCCCTGGACTGGTTCCTGCGGCCCACCCGGGGCACTGACAAGGTGCCCAACAACAGCAGCGC
  ATGGGGCCGGACCGATCTGCTGCCCACCGGCGCCCTCCAGGACAACCAGAATTGGGCATTCCCCAGCCAC
  AGCGTGGAGACCCTGCCCGCCCTGCAGGGGGGCCTCTGGGAGGACAACGACGAGACCACCCAGACCGTGG
  ACGGGCAGCGGTGCTTCCGGATGCCCAAGTTCTTCCCCCCCCCCATGTGCCGGGCCCCCTACAACGAGTG
  CGGCGTCGAGCTGCCCGGGGGCGACTCCAGCGACGAAGACGAAAGCGGGCGGCCCCGGCGGATCGCCAAC
  CGGATCGGCGATACCCCCGAGACACCCTGCAACTCCGAAAACGAGGATGACACCACCGTCGAGGGCACCA
  GCGGGGGACCCGAGGCTATGGACAGCCAGGCCCCCTACCCCAGCGAGGACAGCCCCACAACCGAGAAGGA
  AGCTTGGCTGCAACGGGGCCGGCGGGCCAAGGCCATGCACGCTCGGGGACACACACTCAAACAAGTGCCC
  ATCCCCGAGCCCGACCAAATGGGCGATGACGGCCCAGACCCCGGCCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101339 representing YP_081607
  Red=Cloning sites Green=Tags
  
  MSLLTKAAAEAWGTYLRQRDERCEDAIRCDYGVFQFRNTVFQKTLSMLQGLYLRQYDPPALRTYVQRHQG
  TTVALRNPANWFLVMREQAAIPQIYARSLAADYLCCDDTLEAVGVLAVRPPDSDLTRNTKQAQELPCVLM
  LSHYGTVYVYDWETDGLYEVASDIKAFSKNGLLWCEYVYRHPQTPFATTEPRYHVQKFLCTDPTDAAAVA
  KTAREMNGLNLVIRTPGRTEVEPLLMLGSIEGLRACRPFDHMPAADFRDLLNFIRQRLCCEWYVVGLVGY
  YLAYGPFVPSGMVLLDKFGVVYLHKIEDSDLYRIADNFHMFLKCGLLKLRGLCRFDRGLRGECRLEELPV
  CHHTLKRDVLRWHGALGTITRSQLESALDWFLRPTRGTDKVPNNSSAWGRTDLLPTGALQDNQNWAFPSH
  SVETLPALQGGLWEDNDETTQTVDGQRCFRMPKFFPPPMCRAPYNECGVELPGGDSSDEDESGRPRRIAN
  RIGDTPETPCNSENEDDTTVEGTSGGPEAMDSQAPYPSEDSPTTEKEAWLQRGRRAKAMHARGHTLKQVP
  IPEPDQMGDDGPDPGP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006273
• ORF Size: 1728 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006273.2, YP_081607
• RefSeq ORF: 1728
• Locus ID: 3077463
• MW: 65.0 kDa