UL148 (NC_006273) Virus Tagged ORF Clone

CAT#: VC101300

Myc-DDK-tagged ORF clone of viral ORF for membrane protein UL148 [Human herpesvirus 5], codon optimized for human cell expression, YP_081568

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UL148
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101300 represents NCBI reference of YP_081568 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCGGCTGCTGTTCACCCTGGTGCTGCTGGCCCTCTACGGACCTTCCGTCGATGCCTCCCGGGACT
  ACGTGCATGTCCGGCTCCTCAGCTATCGGGGCGATCCCCTGGTGTTCAAGCACACATTCAGCGGCGTGCG
  GAGACCATTCACCGAGCTCGGATGGGCCGCATGTCGGGATTGGGACAGCATGCATTGCACCCCATTCTGG
  AGCACCGACCTGGAGCAGATGACCGACAGCGTCCGGCGGTACAGCACAGTGAGCCCCGGCAAGGAGGTGA
  CCCTGCAGCTCCACGGCAATCAAACCGTGCAGCCCAGCTTCCTGAGCTTCACATGCCGGCTCCAGCTCGA
  GCCCGTGGTGGAAAACGTGGGCCTGTACGTGGCCTACGTCGTGAACGACGGCGAGCGGCCCCAGCAGTTC
  TTCACCCCCCAGGTGGACGTGGTGCGGTTCGCACTGTACCTGGAGACACTGAGCCGGATCGTGGAGCCCC
  TCGAGTCCGGGCGGCTGACCGTGGAGTTCGACACCCCCGACCTCGCCCTGGCCCCCGATCTCGTGAGCAG
  CCTGTTTGTGGCCGGACACGGCGAGACCGACTTCTACATGAACTGGACCCTGCGGCGGTCCCAGACACAC
  TACCTCGAGGAGATGGCCCTGCAAGTGGAGATCCTGAAACCACGGGGCGTGCGGCACCGGGCCATCATCC
  ACCACCCCAAGCTGCAGCCCGGCGTCGGCCTGTGGATCGACTTCTGCGTGTACCGGTACAACGCCCGGCT
  GACCAGAGGCTACGTGCGGTACACCCTCAGCCCCAAGGCCCGGCTGCCTGCCAAAGCCGAGGGCTGGCTG
  GTGAGCCTGGACCGGTTCATCGTGCAGTACCTCAACACACTGCTGATCACCATGATGGCTGCCATTTGGG
  CACGGGTGCTGATCACATATCTGGTGTCCCGGCGGAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101300 representing YP_081568
  Red=Cloning sites Green=Tags
  
  MLRLLFTLVLLALYGPSVDASRDYVHVRLLSYRGDPLVFKHTFSGVRRPFTELGWAACRDWDSMHCTPFW
  STDLEQMTDSVRRYSTVSPGKEVTLQLHGNQTVQPSFLSFTCRLQLEPVVENVGLYVAYVVNDGERPQQF
  FTPQVDVVRFALYLETLSRIVEPLESGRLTVEFDTPDLALAPDLVSSLFVAGHGETDFYMNWTLRRSQTH
  YLEEMALQVEILKPRGVRHRAIIHHPKLQPGVGLWIDFCVYRYNARLTRGYVRYTLSPKARLPAKAEGWL
  VSLDRFIVQYLNTLLITMMAAIWARVLITYLVSRRR
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006273
• ORF Size: 948 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006273.2, YP_081568
• RefSeq ORF: 948
• Locus ID: 3077479
• MW: 36.5 kDa