FGAM-synthase (NC_009334) Virus Tagged ORF Clone

CAT#: VC101013

Myc-DDK-tagged ORF clone of viral ORF for BNRF1 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129438

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FGAM-synthase
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101013 represents NCBI reference of YP_001129438 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGACAGAGGGCGGGAGACTCAGATGCCCGTGGCCCGCTATGGTGGACCTTTCATTATGGTCAGGT
  TGTTCGGCCAGGACGGCGAGGCCAATATCCAGGAACAGCGCCTTTACGAACTGCTGTCAGATCCAAGATC
  AGCTCTCGGCTTGGACCCAGGACCACTGATCGCAGAAAACCTGTTGCTTGTGGCATTGCGCGGAACAAAT
  AACGATCCCAGGCCACAACGACAGGAGCGCGCCCGAGAGCTGGCCTTGGTTGGGATACTCCTGGGCAACG
  GTGAGCAGGGTGAGCACCTTGGAACCGAGTCCGCATTGGAAGCTAGTGGTAACAATTACGTCTATGCCTA
  TGGGCCAGATTGGATGGCCCGGCCTAGCACATGGTCTGCTGAAATCCAACAGTTCCTGAGACTGCTCGGG
  GCCACATACGTTCTGAGAGTAGAGATGGGTCGGCAGTTTGGCTTTGAAGTTCATAGATCACGCCCTTCCT
  TTAGGCAGTTTCAAGCTATTAATCATCTCGTTCTTTTTGACAACGCACTGCGCAAATACGACTCTGGGCA
  GGTTGCCGCCGGTTTTCAAAGAGCCCTGCTTGTCGCCGGACCAGAAACTGCCGACACCCGGCCAGATCTT
  AGGAAACTTAACGAGTGGGTGTTCGGGGGTCGAGCTGCTGGAGGCCGCCAGCTCGCCGACGAACTCAAAA
  TAGTCTCCGCACTGAGGGACACCTATTCCGGACACCTCGTCCTGCAGCCAACTGAAACACTGGACACCTG
  GAAGGTGCTGTCTCGCGATACCAGAACAGCGCACTCCCTTGAACATGGTTTCATACACGCCGCCGGTACG
  ATCCAGGCCAATTGTCCACAGCTGTTTATGAGAAGACAGCATCCGGGCCTCTTTCCGTTCGTGTCAGCTA
  TAGCCAGCTCACTCGGGTGGTACTACCAGACGGCAACCGGTCCCGGCGCCGACGCACGCGCAGCAGCCAG
  ACGCCAGCAAGCATTTCAAACACGAGCTGCCGCAGAGTGCCATGCAAAAAGTGGAGTCCCTGTTGTGGCA
  GGCTTTTACAGGACTATCAACGCCACACTTAAGGGAGGCGAGGGCCTTCAGCCAACAATGTTCAACGGGG
  AACTCGGTGCAATAAAGCACCAAGCCCTGGATACCGTTCGGTACGATTACGGGCACTATCTTATCATGCT
  GGGTCCCTTTCAGCCTTGGTCCGGTTTGACTGCTCCACCTTGTCCATACGCAGAATCATCCTGGGCACAA
  GCCGCAGTTCAGACTGCCCTTGAACTTTTTAGTGCGCTGTATCCAGCCCCTTGTATTTCAGGATACGCCA
  GGCCCCCGGGACCCTCAGCTGTGATTGAACATCTGGGAAGCTTGGTTCCAAAAGGGGGTCTCCTTCTCTT
  TCTCTCACACCTCCCAGACGACGTGAAGGACGGTCTGGGCGAGATGGGGCCAGCTCGAGCAACAGGGCCA
  GGAATGCAACAGTTCGTGTCATCATATTTCCTCAACCCCGCCTGCAGCAATGTGTTCATCACCGTGCGAC
  AAAGAGGAGAAAAAATTAATGGACGCACCGTACTGCAGGCCCTGGGAAGAGCCTGTGACATGGCAGGGTG
  TCAGCACTACGTCCTGGGGAGTACCGTTCCCCTCGGTGGCCTGAATTTTGTGAATGATTTGGCGTCCCCT
  GTGTCAACCGCGGAGATGATGGATGATTTCTCCCCCTTCTTTACTGTGGAATTTCCTCCCATTCAAGAAG
  AAGGTGCCCGAAGCCCAGTTCCACTCGACGTGGACGAGAGCATGGACATCAGCCCTTCTTATGAACTCCC
  ATGGCTGTCACTGGAGTCCTGTCTCACCTCCATACTCTCCCACCCCACCGTCGGCAGTAAAGAGCACTTG
  GTACGACATACAGATCGCGTGTCAGGCGGCCGAGTGGCACAGCAACCCGGGGTGGGTCCCCTCGACCTCC
  CCCTCGCTGATTACGCTTTCGTTGCCCACTCACAGGTCTGGACCAGGCCTGGCGGTGCCCCCCCACTTCC
  ATACCGAACTTGGGACCGCATGACGGAAAAACTGCTCGTGTCCGCAAAGCCTGGGGGAGAGAACGTTAAG
  GTGAGTGGCACTGTCATCACACTGGGGGAACAAGGCTATAAAGTATCCTTGGACCTCAGAGAGGGAACGC
  GGCTGGCCATGGCCGAGGCGCTGCTTAACGCAGCTTTTGCCCCAATACTGGACCCCGAGGACGTACTCCT
  GACCCTTCACTTGCACTTGGATCCACGCCGGGCGGACAATTCAGTGGTGATGGAAGCAATGACCGCCGCA
  TCTGACTACGCTAGGGGCCTGGGCGTGAAGCTGACATTTGGGAGCGCAAGTTGTCCTGAAACCGGAAGCA
  GTGCATCATCTTTTATGACAGTGGTCGCGTCAGTTTCAGCTCCAGGTGAGTTCTCTGGACCTCTGATCAC
  ACCCGTGCTGCAAAAAACGGGTTCTTTGCTGATTGCTGTCCGCTGTGGAGACGGCAAAATCCAGGGTGGA
  TCTCTTTTTGAGCAACTGTTCTCCGACGTCGCTACAACCCCCCGCGCACCAGAGGCCCTGTCCCTGAAAA
  ATCTGTTTAGGGCCGTCCAGCAGCTGGTGAAGTCTGGAATTGTCTTGTCCGGACACGACATCTCCGATGG
  CGGCCTCGTCACCTGTCTCGTGGAGATGGCCTTGGCAGGTCAGAGGGGAGTAACAATTACAATGCCCGTG
  GCGAGCGACTATTTGCCCGAAATGTTTGCCGAACACCCGGGTTTGGTGTTCGAGGTGGAAGAGAGGTCTG
  TGGGCGAGGTACTGCAGACTCTCAGATCCATGAATATGTACCCCGCAGTACTGGGCAGAGTGGGCGAGCA
  AGGCCCAGATCAGATGTTCGAGGTTCAGCATGGACCCGAAACTGTGCTGCGCCAATCACTCCGACTTCTC
  CTGGGAACATGGAGCAGCTTTGCCTCCGAGCAATACGAGTGCCTCCGACCTGACAGAATTAATCGCAGTA
  TGCACGTCTCCGACTATGGATATAATGAAGCCCTGGCAGTTTCACCCTTGACTGGTAAAAATCTGAGCCC
  TCGCAGACTGGTGACAGAGCCGGATCCAAGATGCCAGGTGGCCGTGCTGTGTGCTCCCGGGACAAGAGGA
  CATGAGAGCCTGCTGGCCGCTTTCACAAACGCAGGTTGTCTTTGCCGCAGGGTGTTCTTCCGGGAGGTTC
  GGGATAATACATTCCTGGACAAGTACGTCGGGCTCGCAATCGGAGGGGTGCACGGGGCTCGCGACTCAGC
  CTTGGCGGGCAGAGCGACAGTCGCTCTGATAAATCGCTCTCCCGCCCTGAGAGACGCCATTCTGAAGTTC
  CTGAACCGGCCGGACACTTTCAGTGTTGCCCTGGGCGAATTGGGGGTACAGGTCCTGGCAGGGCTTGGCG
  CCGTGGGCTCAACCGACAATCCACCAGCCCCCGGGGTGGAGGTCAACGTACAGCGGAGCCCTTTGATACT
  CGCTCCTAACGCCTCTGGAATGTTCGAGAGCAGGTGGCTGAACATCAGCATACCTGCCACCACTAGTAGC
  GTTATGCTCCGGGGCCTCAGGGGATGCGTGCTGCCATGTTGGGTGCAGGGCTCATGTCTGGGCCTCCAGT
  TTACCAACCTCGGTATGCCATATGTCCTCCAGAATGCCCACCAGATCGCGTGCCACTTTCATAGTAACGG
  GACCGACGCCTGGCGCTTTGCGATGAATTATCCCAGAAACCCAACCGAACAGGGCAATATCGCGGGCCTC
  TGCAGTAGGGATGGGAGGCATCTCGCTCTGCTGTGCGATCCGAGCCTGTGCACCGATTTTTGGCAGTGGG
  AACATATTCCCCCCGCCTTCGGACACCCTACCGGCTGTAGCCCTTGGACTTTGATGTTTCAGGCAGCACA
  CCTGTGGAGTCTCCGGCATGGGCGGCCATCCGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101013 representing YP_001129438
  Red=Cloning sites Green=Tags
  
  MEDRGRETQMPVARYGGPFIMVRLFGQDGEANIQEQRLYELLSDPRSALGLDPGPLIAENLLLVALRGTN
  NDPRPQRQERARELALVGILLGNGEQGEHLGTESALEASGNNYVYAYGPDWMARPSTWSAEIQQFLRLLG
  ATYVLRVEMGRQFGFEVHRSRPSFRQFQAINHLVLFDNALRKYDSGQVAAGFQRALLVAGPETADTRPDL
  RKLNEWVFGGRAAGGRQLADELKIVSALRDTYSGHLVLQPTETLDTWKVLSRDTRTAHSLEHGFIHAAGT
  IQANCPQLFMRRQHPGLFPFVSAIASSLGWYYQTATGPGADARAAARRQQAFQTRAAAECHAKSGVPVVA
  GFYRTINATLKGGEGLQPTMFNGELGAIKHQALDTVRYDYGHYLIMLGPFQPWSGLTAPPCPYAESSWAQ
  AAVQTALELFSALYPAPCISGYARPPGPSAVIEHLGSLVPKGGLLLFLSHLPDDVKDGLGEMGPARATGP
  GMQQFVSSYFLNPACSNVFITVRQRGEKINGRTVLQALGRACDMAGCQHYVLGSTVPLGGLNFVNDLASP
  VSTAEMMDDFSPFFTVEFPPIQEEGARSPVPLDVDESMDISPSYELPWLSLESCLTSILSHPTVGSKEHL
  VRHTDRVSGGRVAQQPGVGPLDLPLADYAFVAHSQVWTRPGGAPPLPYRTWDRMTEKLLVSAKPGGENVK
  VSGTVITLGEQGYKVSLDLREGTRLAMAEALLNAAFAPILDPEDVLLTLHLHLDPRRADNSVVMEAMTAA
  SDYARGLGVKLTFGSASCPETGSSASSFMTVVASVSAPGEFSGPLITPVLQKTGSLLIAVRCGDGKIQGG
  SLFEQLFSDVATTPRAPEALSLKNLFRAVQQLVKSGIVLSGHDISDGGLVTCLVEMALAGQRGVTITMPV
  ASDYLPEMFAEHPGLVFEVEERSVGEVLQTLRSMNMYPAVLGRVGEQGPDQMFEVQHGPETVLRQSLRLL
  LGTWSSFASEQYECLRPDRINRSMHVSDYGYNEALAVSPLTGKNLSPRRLVTEPDPRCQVAVLCAPGTRG
  HESLLAAFTNAGCLCRRVFFREVRDNTFLDKYVGLAIGGVHGARDSALAGRATVALINRSPALRDAILKF
  LNRPDTFSVALGELGVQVLAGLGAVGSTDNPPAPGVEVNVQRSPLILAPNASGMFESRWLNISIPATTSS
  VMLRGLRGCVLPCWVQGSCLGLQFTNLGMPYVLQNAHQIACHFHSNGTDAWRFAMNYPRNPTEQGNIAGL
  CSRDGRHLALLCDPSLCTDFWQWEHIPPAFGHPTGCSPWTLMFQAAHLWSLRHGRPSE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 3954 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009334.1, YP_001129438
• RefSeq ORF: 3954
• Locus ID: 5176185
• MW: 142.9 kDa