BBRF3 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101056

Myc-DDK-tagged ORF clone of viral ORF for BBRF3 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129479

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BBRF3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101056 represents NCBI reference of YP_001129479 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAATCTTCCAAGAACGACACTTTCGTCTACCGGACCTGGTTTAAAACTTTGGTGGTGTATTTTGTCA
  TGTTCGTTATGTCTGCTGTGGTCCCCATCACTGCTATGTTTCCCAATTTGGGCTACCCTTGCTACTTTAA
  CGCACTGGTGGACTATGGTGCCCTTAACCTCACCAACTATAATCTGGCACATCATCTGACTCCTACCCTG
  TACCTCGAGCCACCGGAGATGTTCGTCTATATCACACTGGTCTTCATCGCAGACTGTGTGGCATTTATTT
  ATTACGCATGTGGGGAAGTGGCACTGATTAAAGCCCGCAAAAAGGTGTCAGGCCTGACAGACCTCTCCGC
  CTGGGTGTCAGCCGTGGGGTCCCCGACCGTTCTGTTCCTGGCCATTTTGAAACTCTGGAGCATACAGGTG
  TTCATTCAGGTGCTGTCTTACAAGCACGTTTTCCTCTCAGCCTTCGTCTATTTCCTGCACTTCCTTGCCT
  CCGTGCTGCACGCCTGCGCATGCGTGACAAGATTCTCCCCTGTCTGGGTCGTTAAAGCCCAGGACAATTC
  CATCCCTCAGGACACATTTCTGTGGTGGGTTGTCTTCTACTTGAAGCCAATCGTAACCAACCTTTACCTT
  GGATGTCTGGCCCTGGAAACTCTGGTCTTCTCCCTCAGCGTATTTCTGGCCCTGGGGAATTCTTTTTATT
  TCATGGTCGGGGACATGGTGCTGGGCGCAGTGAACCTTTTTCTTGTCCTGCCCATTTTTTGGTACATACT
  GACCGAGGTGTGGCTGGCTTCATTTCTTCGACACAATTTTGGCTTCTACTGTGGCATGTTCATCGCGTCC
  ATCATTCTCATACTTCCTCTTGTTCGATACGAGGCTGTGTTCGTCTCCGCCAAGCTGCACACTACCGTGG
  CCATTAATGTCGCGATCATACCGATCCTCTGCTCTGTGGCTATGCTCATACGGATTTGCAGGATTTTCAA
  GAGTATGCGCCAGGGCACCGATTACGTCCCCGTCAGCGAAACCGTAGAGTTGGAGCTGGAATCCGAACCA
  AGGCCCCGGCCATCTCGGACCCCTAGTCCAGGTAGGAATCGACGGAGATCCTCAACAAGTTCTAGCTCTT
  CCCGGTCCACAAGGCGACAGAGGCCTGTGTCCACCCAAGCTCTGATAAGCAGCGTGCTGCCGATGACAAC
  CGATTCAGAAGAGGAGATATTCCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101056 representing YP_001129479
  Red=Cloning sites Green=Tags
  
  MKSSKNDTFVYRTWFKTLVVYFVMFVMSAVVPITAMFPNLGYPCYFNALVDYGALNLTNYNLAHHLTPTL
  YLEPPEMFVYITLVFIADCVAFIYYACGEVALIKARKKVSGLTDLSAWVSAVGSPTVLFLAILKLWSIQV
  FIQVLSYKHVFLSAFVYFLHFLASVLHACACVTRFSPVWVVKAQDNSIPQDTFLWWVVFYLKPIVTNLYL
  GCLALETLVFSLSVFLALGNSFYFMVGDMVLGAVNLFLVLPIFWYILTEVWLASFLRHNFGFYCGMFIAS
  IILILPLVRYEAVFVSAKLHTTVAINVAIIPILCSVAMLIRICRIFKSMRQGTDYVPVSETVELELESEP
  RPRPSRTPSPGRNRRRSSTSSSSSRSTRRQRPVSTQALISSVLPMTTDSEEEIFP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 1215 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009334.1, YP_001129479
• RefSeq ORF: 1215
• Locus ID: 5176222
• MW: 45.9 kDa