BLRF2 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101038

Myc-DDK-tagged ORF clone of viral ORF for BLRF2 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129461

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BLRF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101038 represents NCBI reference of YP_001129461 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGCACCTCGGAAAGTGCGGCTCCCAAGTGTGAAAGCAGTCGATATGTCAATGGAAGACATGGCTG
  CTAGACTGGCAAGGTTGGAATCCGAGAACAAGGCACTGAAACAGCAGGTGCTGAGAGGAGGCGCCTGCGC
  TTCTAGTACTAGCGTGCCCTCCGCCCCCGTGCCGCCCCCAGAACCCCTTACTGCGAGACAGCGCGAAGTG
  ATGATTACACAGGCTACAGGTCGATTGGCAAGTCAGGCTATGAAGAAGATCGAGGACAAAGTTCGAAAGA
  GCGTGGACGGCGTTACTACTCGGAACGAGATGGAGAACATACTGCAAAATCTGACCCTGCGAATCCAGGT
  CTCCATGCTGGGCGCGAAGGGCCAGCCCAGTCCTGGCGAGGGGACTAGGCCTCGCGAAAGCAATGATCCA
  AATGCGACACGACGCGCTCGGAGCAGAAGTAGAGGCAGGGAAGCCAAGAAGGTACAGATTTCCGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101038 representing YP_001129461
  Red=Cloning sites Green=Tags
  
  MSAPRKVRLPSVKAVDMSMEDMAARLARLESENKALKQQVLRGGACASSTSVPSAPVPPPEPLTARQREV
  MITQATGRLASQAMKKIEDKVRKSVDGVTTRNEMENILQNLTLRIQVSMLGAKGQPSPGEGTRPRESNDP
  NATRRARSRSRGREAKKVQISD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 486 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009334.1, YP_001129461
• RefSeq ORF: 486
• Locus ID: 5176224
• MW: 17.7 kDa