BFLF2 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101015

Myc-DDK-tagged ORF clone of viral ORF for BFLF2 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129443

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BFLF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101015 represents NCBI reference of YP_001129443 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCAGTTACACCAGACGCAGTGAATGCCAGACAGCAGAGGCCCGCTGACCCTGCCCTGAGGCGAC
  TGATGCATCCTCACCACCGCAATTATACTGCCTCCAAGGCGTCCGCGCATTCTGTCAAAAGCGTCTCCAG
  ATGCGGAAAGAGCAGATCCGAGCTGGGGAGAATGGAGCGCGTGGGAAGTGTGGCTAGGAGTATCTGCAGT
  AGGCATACCAGACATGGGGTCGACCGCTCCCACTTTAGCCTGCGCGACTTTTTTAGAGGCATAAGTGCCA
  ACTTTGAGTTGGGGAAAGACTTCCTCAGAGAAATGAACACTCCAATCCATGTGAGTGAGGCCGTGTTTCT
  GCCCCTCAGCCTCTGCACGCTTTCACCAGGACGCTGTCTGAGACTGAGCCCATTTGGACACTCACTGACG
  TTGGGGTCTCATTGTGAAATTTGTATTAACAGATCTCAAGTCCACGTGCCCCAAGAGTTCTCTTCTACCC
  AACTGTCTTTTTTTAATAACGTGCACAAGATTATCCCGAACAAAACCTTCTACGTTAGCTTGCTCAGTAG
  CAGCCCTAGTGCCGTGAAGGCTGGGCTGTCCCAGCCATCACTGCTTTACGCTTACCTCGTGACAGGACAT
  TTCTGTGGCACCATTTGCCCGATCTTTAGCACTAATGGCAAGGGAAGACTTATTATGCATCTTCTGCTCC
  AAGGTACCAGTCTTCACATACCCGAGACTTGTCTGAAGCTCTTGTGCGAGAACATTGGCCCGACCTATGA
  GCTCGCTGTCGATCTGGTGGGCGACGCCTTTTGTATTAAGGTGTCTCCACGGGACACTGTTTATGAGAAA
  GCAGTCAATGTGGATGAGGACGCCATTTATGAAGCCATCAAAGATCTCGAATGTGGTGACGAACTGCGCC
  TCCAAATCATCAATTACACGCAACTGATTCTGGAGAATAAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101015 representing YP_001129443
  Red=Cloning sites Green=Tags
  
  MAPVTPDAVNARQQRPADPALRRLMHPHHRNYTASKASAHSVKSVSRCGKSRSELGRMERVGSVARSICS
  RHTRHGVDRSHFSLRDFFRGISANFELGKDFLREMNTPIHVSEAVFLPLSLCTLSPGRCLRLSPFGHSLT
  LGSHCEICINRSQVHVPQEFSSTQLSFFNNVHKIIPNKTFYVSLLSSSPSAVKAGLSQPSLLYAYLVTGH
  FCGTICPIFSTNGKGRLIMHLLLQGTSLHIPETCLKLLCENIGPTYELAVDLVGDAFCIKVSPRDTVYEK
  AVNVDEDAIYEAIKDLECGDELRLQIINYTQLILENKQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 954 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009334.1, YP_001129443
• RefSeq ORF: 954
• Locus ID: 5176194
• MW: 35.4 kDa