BHRF1 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101020

Myc-DDK-tagged ORF clone of viral ORF for BHRF1 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129442

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BHRF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101020 represents NCBI reference of YP_001129442 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTACAGCACTAGAGAGATTCTGCTGGCTCTGTGCATAAGAGATAGTCGCGTTCATGGAAATGGAA
  CCCTGCACCCCGTGCTTGAACTGGCTGCAAGAGAGACTCCCCTTAGGCTCTCTCCAGAGGATACCGTAGT
  GCTCAGATACCATGTCCTGCTGGAAGAAATTATCGAACGCAACTCTGAGACCTTCACCGAAACTTGGAAT
  CGCTTTATTACGCATACCGAGCATGTTGACTTGGACTTCAACTCCGTTTTCCTCGAGATTTTCCACCGCG
  GCGACCCCTCCCTGGGCAGAGCGCTGGCCTGGATGGCTTGGTGCATGCACGCATGTCGGACCCTCTGCTG
  TAATCAGTCAACACCGTACTACGTGGTAGATCTGTCTGTCAGGGGTATGTTGGAAGCGTCAGAAGGGCTG
  GACGGTTGGATCCATCAGCAGGGAGGATGGTCTACCCTCATCGAGGACAATATCCCAGGATCCCGAAGAT
  TCTCATGGACTTTGTTCCTGGCGGGACTGACGCTGTCACTGCTTGTGATATGCTCCTACCTTTTCATTTC
  AAGGGGCAGACAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101020 representing YP_001129442
  Red=Cloning sites Green=Tags
  
  MAYSTREILLALCIRDSRVHGNGTLHPVLELAARETPLRLSPEDTVVLRYHVLLEEIIERNSETFTETWN
  RFITHTEHVDLDFNSVFLEIFHRGDPSLGRALAWMAWCMHACRTLCCNQSTPYYVVDLSVRGMLEASEGL
  DGWIHQQGGWSTLIEDNIPGSRRFSWTLFLAGLTLSLLVICSYLFISRGRH
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 573 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009334.1, YP_001129442
• RefSeq ORF: 573
• Locus ID: 5176205
• MW: 21.9 kDa