uracil DNA glycosylase (NC_009334) Virus Tagged ORF Clone

CAT#: VC101050

Myc-DDK-tagged ORF clone of viral ORF for BKRF3 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129473

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: uracil DNA glycosylase
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101050 represents NCBI reference of YP_001129473 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATCAAGAGGACTGGATCTGTGGCTCGATGAACATGTGTGGAAACGGAAGCAAGAGATTGGCGTAA
  AAGGCGAAAATCTCCTCCTGCCAGACCTGTGGCTTGACTTCCTGCAGCTCTCACCCATATTCCAGAGGAA
  ACTGGCCGCTGTTATCGCCTGTGTGAGGCGCCTGAGAACTCAGGCAACTGTCTATCCGGAGGAAGATATG
  TGTATGGCTTGGGCCAGATTTTGTGATCCCAGCGACATCAAGGTTGTAATCCTGGGACAGGATCCCTATC
  ATGGAGGGCAGGCTAATGGGCTTGCGTTCTCCATCGCCTACGGGTTCCCTGTACCCCCTTCACTTAGAAA
  TATCTACGCAGAACTCCATCGCTCACTGCCCGAATTTAGTCCCCCTGATCATGGGTGTCTGGACGCATGG
  GCATCACAGGGAGTATTGCTCCTGAACACAATTCTGACAGTGCAAAAGGGCAAGCCGGGTTCTCATGCAG
  ACATCGGCTGGGCCTGGTTCACTGACCACGTTATCAGCCTCCTGTCTGAGCGCCTGAAGGCCTGCGTGTT
  CATGCTCTGGGGCGCTAAGGCAGGAGACAAGGCTTCTTTGATCAACAGTAAAAAGCATCTCGTGCTGACC
  TCTCAGCATCCTTCTCCTCTTGCCCAGAATTCCACTAGGAAAAGCGCACAGCAGAAGTTCCTGGGTAATA
  ACCATTTTGTACTCGCTAACAATTTCCTTCGCGAGAAGGGCCTGGGCGAAATCGACTGGCGGCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101050 representing YP_001129473
  Red=Cloning sites Green=Tags
  
  MASRGLDLWLDEHVWKRKQEIGVKGENLLLPDLWLDFLQLSPIFQRKLAAVIACVRRLRTQATVYPEEDM
  CMAWARFCDPSDIKVVILGQDPYHGGQANGLAFSIAYGFPVPPSLRNIYAELHRSLPEFSPPDHGCLDAW
  ASQGVLLLNTILTVQKGKPGSHADIGWAWFTDHVISLLSERLKACVFMLWGAKAGDKASLINSKKHLVLT
  SQHPSPLAQNSTRKSAQQKFLGNNHFVLANNFLREKGLGEIDWRL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 765 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009334.1, YP_001129473
• RefSeq ORF: 765
• Locus ID: 5176162
• MW: 28.6 kDa