BRLF1 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101044

Myc-DDK-tagged ORF clone of viral ORF for BRLF1 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129468

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BRLF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101044 represents NCBI reference of YP_001129468 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGACCCAAGAAGGACGGTCTGGAGGATTTTTTGAGGTTGACTCCAGAAATTAAGAAGCAGCTCGGTA
  GCCTGGTAAGTGATTATTGCAACGTGCTCAACAAAGAATTCACCGCGGGGTCAGTCGAAATTACATTGCG
  CTCCTACAAAATCTGCAAGGCCTTCATTAATGAAGCGAAGGCTCATGGTCGCGAATGGGGAGGCCTCATG
  GCTACACTGAACATATGCAATTTCTGGGCTATTCTGAGAAATAATCGCGTTCGCAGACGCGCCGAGAACG
  CTGGGAACGACGCCTGTAGTATCGCTTGTCCCATCGTGATGCGGTATGTGCTCGACCACCTGATTGTGGT
  CACCGATAGGTTCTTCATCCAGGCCCCTAGTAATCGCGTGATGATTCCTGCAACAATCGGAACAGCTATG
  TACAAATTGCTTAAACACAGTCGCGTGAGGGCATATACATATAGTAAAGTGCTGGGCGTGGATCGGGCGG
  CCATTATGGCAAGCGGTAAGCAAGTAGTGGAACACCTGAACCGCATGGAGAAAGAGGGCCTCCTGTCTTC
  CAAATTCAAAGCGTTTTGTAAATGGGTCTTCACTTATCCAGTGTTGGAGGAAATGTTTCAAACCATGGTA
  TCATCTAAAACAGGCCACCTCACTGACGACGTCAAAGACGTGAGAGCTCTCATCAAAACCCTGCCCCGCG
  CATCATACAGCTCCCACGCAGGCCAGAGGAGCTATGTTTCTGGGGTTCTGCCAGCATGCCTGCTGAGCAC
  TAAAAGCAAGGCTGTAGAGACGCCTATTCTTGTCAGCGGCGCAGACCGAATGGATGAAGAACTGATGGGC
  AATGATGGTGGAGCATCTCACACCGAGGCCCGGTATTCAGAATCCGGTCAGTTCCACGCTTTCACCGATG
  AGTTGGAAAGTCTGCCATCCCCCACCATGCCGTTGAAACCCGGCGCCCAAAGCGCAGACTGTGGAGATAG
  TAGCTCAAGCTCCTCCGATAGTGGCAATAGCGATACAGAGCAGTCTGAAAGGGAGGAAGCCCGGGCCGAA
  GCACCACGCCTGCGCGCCCCTAAGAGCCGCCGAACCTCTCGACCAAACCGAGGGCAAACACCCTGCCCAA
  GTAATGCTGAGGAGCCGGAGCAGCCATGGATCGCAGCGGTGCACCAGGAGTCAGACGAACGCCCCATCTT
  CCCCCACCCATCTAAACCAACATTTCTGCCGCCAGTGAAAAGGAAAAAAGGTCTGCGAGACTCTCGGGAA
  GGGATGTTCCTGCCTAAGCCAGAGGCGGGGTCAGCTATCTCAGATGTATTTGAAGGGCGGGAGGTCTGCC
  AGCCGAAGAGGATCAGGCCCTTCCATCCCCCTGGTTCTCCCTGGGCTAATAGACCGTTGCCGGCCTCATT
  GGCACCTACCCCCACTGGACCAGTGCATGAGCCCGTAGGCTCCCTGACACCTGCACCCGTCCCTAGACCT
  CTGGATCCAGCGCCCGCAGTTACACCTGAGGCCTCACACCTGCTGGAAGACCCCGATGAGGAAACGTCTC
  AGGCCGTGAAGGCCCTTCGGGAAATGGCCGATACCGTGATCCCCCAGAAAGAAGAAGCTGCCATTTGTGG
  TCAGATGGACCTGAACCACCCTCCTCCTCGCGGTCATTTGGATGAGCTGACAACCACCCTGGAGAGCATG
  ACCGAGGACCTGAATCTGGATTCACCACTGACACCTGAACTCAACGAGATACTCGATACTTTCTTGAATG
  ATGAGTGCCTGCTCCATGCTATGCATATCAGCACCGGCTTGAGCATTTTCGATACATCTTTGTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101044 representing YP_001129468
  Red=Cloning sites Green=Tags
  
  MRPKKDGLEDFLRLTPEIKKQLGSLVSDYCNVLNKEFTAGSVEITLRSYKICKAFINEAKAHGREWGGLM
  ATLNICNFWAILRNNRVRRRAENAGNDACSIACPIVMRYVLDHLIVVTDRFFIQAPSNRVMIPATIGTAM
  YKLLKHSRVRAYTYSKVLGVDRAAIMASGKQVVEHLNRMEKEGLLSSKFKAFCKWVFTYPVLEEMFQTMV
  SSKTGHLTDDVKDVRALIKTLPRASYSSHAGQRSYVSGVLPACLLSTKSKAVETPILVSGADRMDEELMG
  NDGGASHTEARYSESGQFHAFTDELESLPSPTMPLKPGAQSADCGDSSSSSSDSGNSDTEQSEREEARAE
  APRLRAPKSRRTSRPNRGQTPCPSNAEEPEQPWIAAVHQESDERPIFPHPSKPTFLPPVKRKKGLRDSRE
  GMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWANRPLPASLAPTPTGPVHEPVGSLTPAPVPRP
  LDPAPAVTPEASHLLEDPDEETSQAVKALREMADTVIPQKEEAAICGQMDLNHPPPRGHLDELTTTLESM
  TEDLNLDSPLTPELNEILDTFLNDECLLHAMHISTGLSIFDTSLF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 1815 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009334.1, YP_001129468
• RefSeq ORF: 1815
• Locus ID: 5176221
• MW: 66.7 kDa