BRRF1 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101046

Myc-DDK-tagged ORF clone of viral ORF for BRRF1 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129469

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BRRF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101046 represents NCBI reference of YP_001129469 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCCAGCAACCGGGGGAATGCACGCCCACTGAAAAGCTTCCTGCACGAACTGTACCTTAAGCATT
  ATCCTGAAGTAGGGGACGTTGTTCACCTTCTGAATACTATCGGCGTGGATTGCGACCTTCCTCCTAGCCA
  TCCCCTTTTGACCGCCCAGAGAGGTCTTTTCCTTGCACGGGTGCTCCAGGCTGTGCAACAACACAAACTG
  CTGGAGGACACAATCGTGCCCAAAATCTTGAAAAAACTTGCTTACTTTCTCGAACTGCTGTCCTATTACT
  CCCCCAAAGACGAGCAGCGGGATATTGCTGAAGTCCTGGACCACCTGAAAACAAACAGAGATTTGGGGCT
  GGACGACAGACTGTGGGCCTTGATCCGGAAGCTCAGGCAGGACCGACATCATGCTTCTGTCAATGTCCTG
  ATGCCCGGGTCCGATTACACAGCCGTGTCACTGCAGTATTACGACGGCATCAGTATCGGGATGCGGAAGG
  TGATTGCAGACGTGTGTAGATCAGGTTACGCCTCTATGCCATCCATGACAGCAACCCACAACCTCAGCCA
  TCAGCTCCTGATGGCCTCAGGGCCTAGCGAAGAACCCTGCGCTTGGAGAGGCTTCTTCAATCAGGTGCTC
  CTGTGGACCGTGGCTCTGTGCAAGTTTAGACGCTGTATTTACTATAACTACATTCAAGGGTCCATTGCTA
  CCATTTCACAGTTGTTGCACCTTGAGATCAAGGCGCTGTGCTCATGGATTATTAGCCAGGACGGCATGCG
  GCTGTTCCAGCACAGCCGGCCACTTCTGACTCTGTGGGAATCTGTGGCAGCTAACCAAGAGGTGACTGAC
  GCTATTACCTTGCCTGACTGTGCCGAGTACATTGATCTCCTGAAACATACAAAACACGTTCTGGAAAATT
  GCAGCGCTATGCAGTACAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101046 representing YP_001129469
  Red=Cloning sites Green=Tags
  
  MASSNRGNARPLKSFLHELYLKHYPEVGDVVHLLNTIGVDCDLPPSHPLLTAQRGLFLARVLQAVQQHKL
  LEDTIVPKILKKLAYFLELLSYYSPKDEQRDIAEVLDHLKTNRDLGLDDRLWALIRKLRQDRHHASVNVL
  MPGSDYTAVSLQYYDGISIGMRKVIADVCRSGYASMPSMTATHNLSHQLLMASGPSEEPCAWRGFFNQVL
  LWTVALCKFRRCIYYNYIQGSIATISQLLHLEIKALCSWIISQDGMRLFQHSRPLLTLWESVAANQEVTD
  AITLPDCAEYIDLLKHTKHVLENCSAMQYK
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 930 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009334.1, YP_001129469
• RefSeq ORF: 930
• Locus ID: 5176203
• MW: 35.3 kDa