BGLF3.5 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101060

Myc-DDK-tagged ORF clone of viral ORF for BGLF35 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129483

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BGLF3.5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101060 represents NCBI reference of YP_001129483 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAATGAGCTCATCAAAGAGGGATCTGGTGGCTCAGCAACTGAGAGCTTCTGTGGAGAAAAGAGCCG
  CAGTCAGTGCCAGAGACCGCTTCGGGCGGGACCACGCCCTGTTCGAGACCCAGTTTACCTCCGCCAGGGG
  CGCACTGGAAAGCTTGAGGCGCGCGCGCGAAACTTTCGAGAGCAAGCAGCTGATCTCTACATACCAGAGG
  GTGGTCACAGCCACCAAGACCCAATTTCCTAAAATTAACTATAAGCAGCTGGAGCGGGTCGAGGAACTCC
  GCGAGCAGGAACTTGAGGCTCGGGACGAGCTCAGACAGGCGCTCGAGCCATTCGAGGAGCATGGCTGTGA
  GTATGGTTGCGGGGTCGAACCTGATGAACTCCTGCAGCAGTGGCGCGTGGAGTGTCTGCCTCGCACCCCC
  AGCAGGGATCCTGGGCTGTTCGGCGAGGGCGATTGTCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101060 representing YP_001129483
  Red=Cloning sites Green=Tags
  
  MKMSSSKRDLVAQQLRASVEKRAAVSARDRFGRDHALFETQFTSARGALESLRRARETFESKQLISTYQR
  VVTATKTQFPKINYKQLERVEELREQELEARDELRQALEPFEEHGCEYGCGVEPDELLQQWRVECLPRTP
  SRDPGLFGEGDCH
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 459 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009334.1, YP_001129483
• RefSeq ORF: 459
• Locus ID: 5176184
• MW: 17.7 kDa