EBNA-3A (NC_009334) Virus Tagged ORF Clone

CAT#: VC101040

Myc-DDK-tagged ORF clone of viral ORF for EBNA-3A [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129463

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EBNA-3A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101040 represents NCBI reference of YP_001129463 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAAGGATCGCCCTGGTCTGCCCGCGCCCGATGACAATATCGAGGAAGTGCCCTCAACTAGCGGAG
  TGCAAGAGCGCGCCAGTGAGGGGGACTGGGAGAACGTTCTGATTGAGATCTCAGATTCTTCTAGCGAAGA
  AGAGGCTGAGGATGCCCATCTTGAGCCTTCCCAGCGCGGTAAGAAGAGAAAAAGGGTCGATGATGATGCC
  GGGGGCTCCGCACCAGCTCAACACGTGCCCCCACCCCAGCTTGACCACCCCGGTAGAGAGGCAATTTTGT
  ACAGGTTTCCCCTGGACCTTCGGAGATTCATTCAAGCCATAGGCGCTGCTGCTACCCATCCCGATACTCG
  GGCCATCGATCAGTTCTTCGGTAGTCAAATTAGCAACACAGATCTCTACGTAATGTACGCAATGGCTATC
  AGGCAGGCGATTCGCGACCGGCGGCGGAACCCCGCAAGTAGAAGGTCCCAGGTTAAATGGCGCATGACCA
  CACTCGCTGCGGGCTGGCCAATGGGCTACCAGGCTTACTCATCTTGGATGTACTCCTATACTGACCCACA
  GGTCACAGCCACGATCATCCACCTGCAGGCAACCCTGGGATGCGCTTCCGGCAGGAGATGCCATGTCACT
  TTCAGCGCCGGCACCTTTAGGCCCCCACGGTGTTCCCCAGGTGACCGACAGTGGCTCTACGTCCAGTCAA
  GAGTTGGCGATCTTGTTCAGTCTAGCAACCCCTGCTATAGCATCTTCTTTGACTACATGGCTATCCACAG
  ATCCTTGACCAAAATATGGGACGAAGTGGTGACTCCTGACCAGCGGGTGACCTTCATGGAGTTTCTTGGT
  TTCCTCCAGCGGACAGAGCTGGTGTATATAAAATCCTTTGTGAGCTACGCCCTGGGCACGACAAGCATTG
  AGACGCCATGGATGGATGAGAACCCCTCTACAGAGACAGCTCAGGCCTGGAACGCAGGGCTTCTGCGCGG
  CCGCGCATACGGACAGGACCTTCTCCGAACGGAAGGCGAACACGGGGAGGGCGCCACATGCGAGACCAGG
  GAGGAGTCAGAAGATACAGAAAGTGATGGCGATGACGAAGAGCTGCCCCGAGTGGTAAGCCGGGACGGGA
  CTAAACATAGAAGGCCTCCGATTTTCCTGCGCCGACTTCATCGGCTGTTGCTCATGCGAGCAGGTAAGGG
  TAAAGAACGGGCCAGAGAGACACTTGCCAAAGCTCCTCGGAGGACCTACGGGACCCCGAGACCCCCTGTG
  CAGAAACCTAGACCCGAGGTACCACAATCTTATGAAACAGCCACCTCCCACGGCAGTGCGCAGGTCCCAG
  AACCACCCCCTACACATCCCCTGCATCAACAGCATTCCATGGCCCCCTGCATGGTGGCTCAGAATCCCCG
  CGCACCTCTGGGGGATCAGTTGCCTGGTGTGCCAAAGGATGGTCGAGGGGCCTGCGCTCCGGTCCCTGCC
  CTGGCCGGACCGATTGTTCGGCCTTGGGAAAGCAGTTTGCTGCAGTCACCTGGTCGAGCCTTCGCCCCCG
  TCAGCCCTCAACCTATGCCAGTTGAACCTGTGCCTGTGCCAACCGTGGCTCTCGAGAGACCAGTCTGCCC
  GGCTCCGCCCGAGATTGCCATGCAAGGCCCCGGGGAGCCCAGCGGCATTAAAAGAACTCGCGAGCGCTGG
  AGACCTGCCCCCTGGACCCCTAACCCCCCAAGGTCCCCCTCACAAATGTCTGTGCGAGATCGACTTGCCC
  GCCTCCGCGCTGAGGCTCAGGCTCGACAAGCAAGCGTCGAGGTTCAGCCAACCCAGCTGACTCAGGTCTC
  ACCTCAACAGCCAATGGAACGGCCACTGGAGCCAGAACAGCAGATGTTCCCCGGGTCTCCCTTTTCCCAG
  GTCGCTGACGTCGCAAGGGAGTCTGGCGTCCCGGCTATGCAGCCACAGTACTTCGATCTTCCGCTGACTC
  AACCAATTAGCCAGGGCGCCCCCGCAGCTCCTCTCCGCGCAAGCATGGGACCAGTCCCTCCGGTGCCTGC
  CACACAACCACAATATTTCGATATCCCCCTGACCGAACCGATTAACCAAGGCGCTTCAGCTGCTCATTTT
  TTGCCCCAGCAGCCGATGGAAGGTCCGCTCGTGCCAGAGAGGTGGATGTTCCAGGGCGCCACACTTAGTC
  AGTCAGTACGCCCAGGAGTTGCCCAGTCCCAATACTTTGATTTGCCCCTGACTCAGCCTATTAACCACGG
  GGCTCCCGCCGCCCACTTTCTGCACCAACCCCCTATGGAGGGTCCATGGGTGCCTGAGCAATGGATGTTT
  CAGGGAGCTCCCCCATCACAGGGTACCGACGTCGTGCAGCATCAGCTGGACGACCTGGGATATCCACTGC
  ATGATCTTAATCATCCAGGTGTGCCGGTGTCACCTGCTGTGAACCAGTACCACTTCAGCCAGGCTGCATT
  TGGTCTCCCAATCGACGAGGACGAATCAGGTGAACGCAGTGATACAAGTGAGCCCTATGAGGCGTTGGAC
  CTCTCCATACACGGAAGACCATGCCCCCAGGCCCCTGAGTGGCCAGTCCAGGGTGAAGGGGGACAGGATG
  CCACTGAAGTATTGGATCTGTCTATCCATGGCCGCCCACGCCCTCGCACACCCGAATGGCCGGTACAGGG
  AGAAAGTGGTCAAAATGTCACAGACCACGAACCCAGAAGAGTTGTTGTGTCTGCCATAGTGCACATGTGC
  CAAGATGACGAGTTTCCTGACCTCCAGGACCCCCCCGATGAGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101040 representing YP_001129463
  Red=Cloning sites Green=Tags
  
  MDKDRPGLPAPDDNIEEVPSTSGVQERASEGDWENVLIEISDSSSEEEAEDAHLEPSQRGKKRKRVDDDA
  GGSAPAQHVPPPQLDHPGREAILYRFPLDLRRFIQAIGAAATHPDTRAIDQFFGSQISNTDLYVMYAMAI
  RQAIRDRRRNPASRRSQVKWRMTTLAAGWPMGYQAYSSWMYSYTDPQVTATIIHLQATLGCASGRRCHVT
  FSAGTFRPPRCSPGDRQWLYVQSRVGDLVQSSNPCYSIFFDYMAIHRSLTKIWDEVVTPDQRVTFMEFLG
  FLQRTELVYIKSFVSYALGTTSIETPWMDENPSTETAQAWNAGLLRGRAYGQDLLRTEGEHGEGATCETR
  EESEDTESDGDDEELPRVVSRDGTKHRRPPIFLRRLHRLLLMRAGKGKERARETLAKAPRRTYGTPRPPV
  QKPRPEVPQSYETATSHGSAQVPEPPPTHPLHQQHSMAPCMVAQNPRAPLGDQLPGVPKDGRGACAPVPA
  LAGPIVRPWESSLLQSPGRAFAPVSPQPMPVEPVPVPTVALERPVCPAPPEIAMQGPGEPSGIKRTRERW
  RPAPWTPNPPRSPSQMSVRDRLARLRAEAQARQASVEVQPTQLTQVSPQQPMERPLEPEQQMFPGSPFSQ
  VADVARESGVPAMQPQYFDLPLTQPISQGAPAAPLRASMGPVPPVPATQPQYFDIPLTEPINQGASAAHF
  LPQQPMEGPLVPERWMFQGATLSQSVRPGVAQSQYFDLPLTQPINHGAPAAHFLHQPPMEGPWVPEQWMF
  QGAPPSQGTDVVQHQLDDLGYPLHDLNHPGVPVSPAVNQYHFSQAAFGLPIDEDESGERSDTSEPYEALD
  LSIHGRPCPQAPEWPVQGEGGQDATEVLDLSIHGRPRPRTPEWPVQGESGQNVTDHEPRRVVVSAIVHMC
  QDDEFPDLQDPPDEA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 2775 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009334.1, YP_001129463
• RefSeq ORF: 2775
• Locus ID: 5176209
• MW: 102.4 kDa