BORF2 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101029

Myc-DDK-tagged ORF clone of viral ORF for BORF2 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129452

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BORF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101029 represents NCBI reference of YP_001129452 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACCACCTCCCACGTCGAGCACGAACTGCTGAGTAAGTTGATCGACGAGCTGAAAGTTAAAGCCA
  ACTCTGATCCAGAAGCAGATGTGCTCGCCGGTAGGTTGCTGCATAGGCTTAAAGCTGAGAGTGTCACCCA
  TACCGTGGCCGAGTACCTTGAAGTTTTCAGTGATAAATTCTATGATGAGGAATTCTTCCAGATGCATAGG
  GACGAATTGGAGACTCGCGTGTCTGCCTTCGCCCAATCCCCCGCATACGAAAGGATTGTTTCATCTGGAT
  ACTTGAGTGCGCTCAGGTATTACGACACCTACTTGTATGTAGGACGCTCCGGAAAGCAAGAAAGTGTACA
  GCATTTCTACATGAGGCTTGCCGGGTTCTGTGCATCCACTACTTGTCTGTATGCTGGGCTGCGGGCCGCT
  CTGCAGCGGGCAAGGCCTGAAATTGAGAGTGATATGGAGGTCTTCGATTATTACTTTGAGCACCTCACAT
  CTCAGACCGTGTGCTGCTCAACTCCCTTTATGAGATTCGCCGGAGTGGAAAATTCTACACTTGCGTCATG
  TATACTCACCACTCCCGATCTGTCAAGCGAATGGGACGTAACCCAAGCCCTTTATCGCCATTTGGGCCGA
  TATCTTTTTCAGCGCGCCGGTGTAGGGGTAGGTGTTACAGGCGCAGGGCAGGATGGAAAACATATCTCTC
  TGCTTATGCGCATGATCAATAGCCACGTTGAGTACCACAACTACGGCTGCAAGCGGCCCGTGAGCGTGGC
  CGCCTACATGGAACCTTGGCATTCACAAATATTTAAGTTCCTGGAAACCAAGCTGCCTGAGAATCATGAG
  CGCTGCCCCGGTATATTTACAGGGTTGTTCGTGCCTGAGCTCTTCTTCAAGCTGTTTCGCGACACCCCTT
  GGTCTGATTGGTATCTGTTCGATCCTAAGGATGCCGGTGACTTGGAACGGCTTTACGGAGAAGAATTTGA
  AAGAGAATACTACCGACTGGTGACCGCTGGCAAATTCTGCGGCAGGGTGAGTATCAAAAGTCTTATGTTT
  AGTATAGTAAACTGTGCAGTAAAGGCAGGATCCCCCTTTATCCTCCTCAAAGAAGCCTGCAACGCACATT
  TCTGGCGGGACCTTCAGGGGGAGGCGATGAATGCAGCTAACTTGTGCGCCGAAGTTTTGCAGCCGAGCAG
  GAAATCTGTAGCCACCTGTAACCTCGCCAATATCTGTCTGCCTCGCTGTCTCGTGAATGCGCCACTCGCA
  GTTCGAGCCCAAAGGGCAGACACACAAGGAGACGAATTGCTGCTTGCTCTGCCACGACTGTCTGTAACCC
  TGCCCGGCGAGGGGGCTGTGGGGGACGGGTTTTCCCTGGCGAGGCTCCGAGATGCAACACAGTGCGCAAC
  CTTTGTTGTCGCTTGCTCCATCCTGCAGGGCAGCCCCACCTATGACTCCCGGGATATGGCCAGTATGGGC
  CTCGGCGTGCAGGGGCTCGCAGATGTGTTCGCTGACCTGGGCTGGCAATACACTGATCCACCTTCTCGGT
  CCCTGAACAAGGAAATCTTCGAACACATGTATTTCACTGCCCTGTGTACCTCATCTCTGATCGGTCTGCA
  TACCCGGAAGATCTTCCCAGGCTTCAAGCAATCAAAATACGCGGGAGGCTGGTTTCATTGGCATGACTGG
  GCTGGAACGGACCTCAGTATTCCGAGAGAAATATGGAGCAGGCTGTCCGAACGAATCGTACGGGACGGCC
  TGTTTAATAGTCAGTTTATTGCACTCATGCCGACGTCTGGCTGTGCCCAGGTGACTGGTTGTTCCGATGC
  GTTCTATCCCTTCTACGCAAATGCTAGTACTAAGGTGACAAACAAAGAAGAAGCTCTGAGGCCCAATAGA
  TCCTTTTGGCGGCACGTAAGATTGGATGACCGCGAGGCATTGAACCTGGTGGGCGGGCGGGTATCATGTC
  TTCCCGAGGCACTCAGGCAGCGCTACTTGCGCTTCCAGACCGCATTTGACTATAATCAGGAGGACCTGAT
  CCAGATGTCAAGAGACCGCGCCCCCTTTGTCGACCAATCTCAATCACACAGTTTGTTTCTGAGAGAAGAG
  GATGCGGCAAGAGCCAGTACCCTGGCAAACCTCCTGGTGCGGTCCTACGAGTTGGGCCTTAAGACTATCA
  TGTATTATTGCAGAATCGAAAAGGCCGCCGATCTTGGCGTGATGGAGTGTAAAGCCTCTGCTGCATTGTC
  CGTTCCCAGGGAAGAGCAGAACGAGAGATCTCCAGCAGAGCAGATGCCTCCAAGGCCCATGGAACCCGCT
  CAGGTGGCCGGGCCAGTGGACATCATGAGCAAAGGACCAGGTGAGGGACCCGGTGGCTGGTGTGTCCCGG
  GCGGTTTGGAAGTGTGCTACAAGTACCGGCAGCTGTTTAGTGAAGACGATCTGCTGGAAACCGACGGATT
  CACAGAGAGGGCCTGTGAGTCCTGTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101029 representing YP_001129452
  Red=Cloning sites Green=Tags
  
  MATTSHVEHELLSKLIDELKVKANSDPEADVLAGRLLHRLKAESVTHTVAEYLEVFSDKFYDEEFFQMHR
  DELETRVSAFAQSPAYERIVSSGYLSALRYYDTYLYVGRSGKQESVQHFYMRLAGFCASTTCLYAGLRAA
  LQRARPEIESDMEVFDYYFEHLTSQTVCCSTPFMRFAGVENSTLASCILTTPDLSSEWDVTQALYRHLGR
  YLFQRAGVGVGVTGAGQDGKHISLLMRMINSHVEYHNYGCKRPVSVAAYMEPWHSQIFKFLETKLPENHE
  RCPGIFTGLFVPELFFKLFRDTPWSDWYLFDPKDAGDLERLYGEEFEREYYRLVTAGKFCGRVSIKSLMF
  SIVNCAVKAGSPFILLKEACNAHFWRDLQGEAMNAANLCAEVLQPSRKSVATCNLANICLPRCLVNAPLA
  VRAQRADTQGDELLLALPRLSVTLPGEGAVGDGFSLARLRDATQCATFVVACSILQGSPTYDSRDMASMG
  LGVQGLADVFADLGWQYTDPPSRSLNKEIFEHMYFTALCTSSLIGLHTRKIFPGFKQSKYAGGWFHWHDW
  AGTDLSIPREIWSRLSERIVRDGLFNSQFIALMPTSGCAQVTGCSDAFYPFYANASTKVTNKEEALRPNR
  SFWRHVRLDDREALNLVGGRVSCLPEALRQRYLRFQTAFDYNQEDLIQMSRDRAPFVDQSQSHSLFLREE
  DAARASTLANLLVRSYELGLKTIMYYCRIEKAADLGVMECKASAALSVPREEQNERSPAEQMPPRPMEPA
  QVAGPVDIMSKGPGEGPGGWCVPGGLEVCYKYRQLFSEDDLLETDGFTERACESCQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 2478 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009334.1, YP_001129452
• RefSeq ORF: 2478
• Locus ID: 5176186
• MW: 93.0 kDa