BKRF4 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101051

Myc-DDK-tagged ORF clone of viral ORF for BKRF4 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129474

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BKRF4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101051 represents NCBI reference of YP_001129474 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTATGTTTCTCAAGAGCCGAGGTGTCAGAAGCTGCCGGGACCGGAGGCTCCTGTCTGACGAAGAGG
  AGGAGACGTCTCAAAGCTCATCCTACACTCTTGGCAGCCAAGCCTCCCAGAGCATCCAGGAGGAGGATGT
  CTCCGACACTGACGAGAGCGACTACTCCGACGAGGACGAGGAAATTGACCTCGAGGAAGAATATCCAAGC
  GACGAGGATCCGAGCGAAGGATCAGACAGCGATCCGAGCTGGCACCCATCTGACAGCGATGAAAGCGACT
  ATTCTGAGTCAGATGAGGACGAGGCCACGCCAGGTTCCCAGGCATCTAGGAGCAGCAGAGTCAGCCCTTC
  TACCCAGCAGAGCTCTGGCCTGACACCTACACCTTCTTTCAGCAGACCTCGAACACGGGCTCCCCCTAGA
  CCGCCTGCTCCAGCACCAGTGAGGGGGAGAGCCTCTGCCCCACCACGCCCCCCAGCTCCTGTCCAGCAGT
  CAACCAAAGATAAAGGACCCCACCGACCAACCCGACCCGTGTTGAGAGGTCCTGCCCCACGCAGGCCCCC
  TCCACCCTCTTCCCCAAACACCTACAATAAGCATATGATGGAGACAACACCACCTATTAAGGGGAACAAT
  AATTATAATTGGCCGTGGCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101051 representing YP_001129474
  Red=Cloning sites Green=Tags
  
  MAMFLKSRGVRSCRDRRLLSDEEEETSQSSSYTLGSQASQSIQEEDVSDTDESDYSDEDEEIDLEEEYPS
  DEDPSEGSDSDPSWHPSDSDESDYSESDEDEATPGSQASRSSRVSPSTQQSSGLTPTPSFSRPRTRAPPR
  PPAPAPVRGRASAPPRPPAPVQQSTKDKGPHRPTRPVLRGPAPRRPPPPSSPNTYNKHMMETTPPIKGNN
  NYNWPWL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 651 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009334.1, YP_001129474
• RefSeq ORF: 651
• Locus ID: 5176197
• MW: 24.0 kDa