helicase-primase primase subunit BSLF1 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101034

Myc-DDK-tagged ORF clone of viral ORF for BSLF1 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129457

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: helicase-primase primase subunit BSLF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101034 represents NCBI reference of YP_001129457 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGCTCCAGTGGTGATCAAGGCTCTCGTTGCTTCAAACACAGATATTGCAGAGGCCATCCTCGATG
  CCATCCTGTCCAGACCTGACGAAGGGTTTAGATTGTTCTGTCTGTGCCATAATGCCTCCCCTCTGCACCA
  CGTCGCCGGCTCACTGGTGGAGCTGCAGCTGCATCTGCCCAAAAAGCGGTTGACCTCTCAGAGTCGGTGT
  GGGCTCGTGCTCACCCTCCATCTGCCAGCGGAGGAGGCCTTTCCGTTCTTGCGAGGACTTACGCCACTGA
  CTGCCGATAGACTGTCAACCTATCTGGATAGAGCGGGCGCCCTGAGAAGTCTTACACCTCTGGTTGAATT
  GCTCACCCTCAGCGCGAAGAAACAGCCCCAGGGCGACGCGCGGGGCCGCGTAGCCTGGCTCAGACCAAAG
  ATCGTGGGCTGTCTTCGCAGAATCTATAGAGTGAATATCAGCGCACGCTGGTTCATCAGCACCTTCGGGA
  GTCACGAGGCACAGTTTGTGCTCGTGACTGCAGCATATTATTTCTGGGGAATTCCATGTACAATAGAGAC
  CCTGGCGCATCTGACTGAGTTGTTTACTTCAGAAAGCGGGCAGAGTCTGGCGGCGGTGACCAGTCTGGCA
  GAACTCGGAGAAGTGTTCGGATCCTCTGCTTGGGCCGAGCAAACTGAGGCCTTCGCCCATTTTGCCCATG
  AAAAATTGAGGCGAGACAGCCGCGAAATTCGCGCCGTGGCTAGAACTATAGACGCCTATCGAGGTAGACT
  TCCACTCGCCAGCGCTGACCTGGTGCGGTACGTGTACCTCGCCCACGCTCAGTGTTTCAATGAGGGGACT
  TTTAAGAGGTATTCACAGTTGACCAGTGTCGGGGAGATTGGCTGTCTGCCAAGTGGTGGAGTCGTTCTGC
  CAAGTCTGCTTGACAGGGGCTTCGCTGAGCACATGCGGACGTATTTCACACGGGAAACCTACTTGGCAGA
  ACACGTGAGAGTTCAGCAGCTTAAAATTAGAATGGAGCCACCAGCCCCGTACACATGGGATCCTGACCCC
  GACGACGGTCTGATGAGAGCCTGGGCCGGCCTGTCCGTCGACGTAGCCAGGGAACTCGTTGAATTGGCGC
  GGTGGCATGCGGACGAAGGGCCTACATATCCCCCAACGCTTCAGGGCTTTCTCTGTCTGGCTGGCCAGGC
  AACCTGCAGAGGACAGTGGAACCCCAAAGAACAGTTTCTGCCGCCGACGGTGTTGAGGCGGGTGCAAAGA
  TTGCCCGTGTTCCTCTGCCACTTCGCTGATAGGCACTACTTCGTGATGACCGCCGCCGATCCATTCAGTT
  CACATCTCGCTGAAGTGGTCTCAACACCCACGAACTGCAGGCTGCCAGACACCTGCCTCACAAGAGCCCT
  CAGTTATACACCCGTGTATTACAGCCAAAACTCTCTTAGCGAACAGCTGTTTGTCAGCAGGCACGAGTAT
  TTTAATCCACGCCTGCCAGTGTGCAATCTGGTGCTTGATCTGGATCTCAAGATCAAGGGAGCACCATGGT
  CTCTGGAAGAGATATATGATCTCTGCCGAACTGTGCGCCGGGAAGTACTGAGGCTGATGAGAAGGCTTGG
  GCCAGTGAGCAGAGCCCATCCCGTCTATTTCTTCAAAAGCGCTTGTCCTCCAGCCGACCCAGATAATATG
  GAAGACGTTCTCCCATTTTGTATCTGCACGGGCAAATTGGGTTTCAGGGTCATCACTCCTCTCCCTCGCG
  GCCACGCGATCGTGGGGACGAGCGCCGTCCAGGGGTTCGTGTCTGTGCTGCAAAAACTCATGGGTCTCAC
  TGCTTGCTTGAGGAGGATGAGGCATAAGATTAAGGAGATCGGAGCTCCTTTGTTTGATTCAGGAGTATAT
  CACGCCGGAAGATGCATCAGGCTGCCGCACACCTACAAGGTTGATCGGGGCGGCGGTCTTTCAAGGCAGC
  TGCGCCTGTTTGTGTGCCACCCAGAAGAGGAGGACAAACATTCCTATGTTAAGAACGCTCTCAACATACA
  AAATCTCCTTCACCACAGTCTGCATGTCGGTTGGCCCGCACCCAAGACTTTCTGTTACCATATTGCCGAC
  GATGGACGCGACTATCTTATTCAGAGGACACGCGAGACACTGCCTCCCACTGTCGAGAATGTCTGCGCGA
  TGATAGAAGGGCACCTGGGGTTGGATTTGGTGGCTTGGGTTTCTAGCTGTATTTGGCCTTCCCTGATGTC
  AACCCTTGCCACTGCAGTCCCAGAAGATAAGTTCCCCCAGTTTCTCCACGTAACATTTGAGCAGACAGGC
  CCCAATCTGGTACAGGTATGCCATGCGAGAGGCCGAAATTTCGCGTGTCTCCGCCACACGCATAGAGCGT
  CTTCAAAGAACGTTCGGGTGTTTCTTGTGCTGTACTATACGTCCCAGGCCATTACGGTTACCTTCATGTC
  TCAATGTTTTGCGGGCCGATGCGGAGCAAACCAGCCCACCGCGCACTTCTCTATAAGTGTCCCAGCGTCC
  AGAATAATCAACAGGGCCGAGGCCAGCCAGGATTCCACTACCAGCCAACTTGCCAGGCGCCGGGATAGGC
  AGGACGGCAGTTTCAGTGAGACTCTGCCTAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101034 representing YP_001129457
  Red=Cloning sites Green=Tags
  
  MSAPVVIKALVASNTDIAEAILDAILSRPDEGFRLFCLCHNASPLHHVAGSLVELQLHLPKKRLTSQSRC
  GLVLTLHLPAEEAFPFLRGLTPLTADRLSTYLDRAGALRSLTPLVELLTLSAKKQPQGDARGRVAWLRPK
  IVGCLRRIYRVNISARWFISTFGSHEAQFVLVTAAYYFWGIPCTIETLAHLTELFTSESGQSLAAVTSLA
  ELGEVFGSSAWAEQTEAFAHFAHEKLRRDSREIRAVARTIDAYRGRLPLASADLVRYVYLAHAQCFNEGT
  FKRYSQLTSVGEIGCLPSGGVVLPSLLDRGFAEHMRTYFTRETYLAEHVRVQQLKIRMEPPAPYTWDPDP
  DDGLMRAWAGLSVDVARELVELARWHADEGPTYPPTLQGFLCLAGQATCRGQWNPKEQFLPPTVLRRVQR
  LPVFLCHFADRHYFVMTAADPFSSHLAEVVSTPTNCRLPDTCLTRALSYTPVYYSQNSLSEQLFVSRHEY
  FNPRLPVCNLVLDLDLKIKGAPWSLEEIYDLCRTVRREVLRLMRRLGPVSRAHPVYFFKSACPPADPDNM
  EDVLPFCICTGKLGFRVITPLPRGHAIVGTSAVQGFVSVLQKLMGLTACLRRMRHKIKEIGAPLFDSGVY
  HAGRCIRLPHTYKVDRGGGLSRQLRLFVCHPEEEDKHSYVKNALNIQNLLHHSLHVGWPAPKTFCYHIAD
  DGRDYLIQRTRETLPPTVENVCAMIEGHLGLDLVAWVSSCIWPSLMSTLATAVPEDKFPQFLHVTFEQTG
  PNLVQVCHARGRNFACLRHTHRASSKNVRVFLVLYYTSQAITVTFMSQCFAGRCGANQPTAHFSISVPAS
  RIINRAEASQDSTTSQLARRRDRQDGSFSETLPN
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 2622 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009334.1, YP_001129457
• RefSeq ORF: 2622
• Locus ID: 5176218
• MW: 98.0 kDa