BALF3 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101085

Myc-DDK-tagged ORF clone of viral ORF for BALF3 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129509

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BALF3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101085 represents NCBI reference of YP_001129509 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGGACTCCTGGCAGCAGCTTATAGTCAAGTCTATGCCCTGGCTGTAGAATTGTCCGTCTGCGCAC
  GCCTGGATCCCCGAAGCCTCGACGTGGCTGCTGTTGTAAGAAATGCAGGACTGCTGGCCGAGCTGGAGGC
  AATACTGCTTCCACGCCTGCGCAGGCAAAACGATCGCGCATGCAGTGCTCTCTCCCTGGAGCTTGTGCAT
  CTTTTGGAGAATTCAAGAGAGGCCTCCGCCGCCCTTTTGGCCCCCGGGCGGAAGGGGACACGGGTCCCGC
  CACTTAGAACGCCGAGCGTTGCCTACTCCGTGGAATTTTATGGCGGCCACAAGGTAGACGTGTCCCTGTG
  CCTGATTAATGATATCGAGATTCTCATGAAGAGGATTAACTCCGTGTTCTATTGTATGTCCCATACCATG
  GGGCTGGAGAGCCTCGAACGCGCACTGGACCTCCTGGGGAGGTTCAGAGGGGTGAGTCCTATTCCCGATC
  CACGACTGTACATAACCTCTGTCCCGTGCTGGCGCTGCGTCGGAGAACTGATGGTGCTGCCCAACCACGG
  CAATCCCTCCACCGCAGAAGGCACCCACGTGTCCTGCAACCATTTGGCTGTTCCTGTAAATCCTGAGCCA
  GTGAGCGGACTGTTCGAAAACGAGGTACGCCAGGCCGGTTTGGGTCATTTGCTTGAAGCCGAAGAAAAGG
  CCCGGCCCGGAGGACCTGAGGAGGGCGCAGTACCCGGCCCCGGCAGACCTGAGGCTGAGGGCGCTACACG
  AGCCCTTGACACGTACAACGTTTTCTCCACAGTGCCTCCTGAGGTAGCCGAGCTGAGCGAGTTGTTGTAC
  TGGAATTCCGGCGGCCATGCAATCGGGGCCACCGGACAGGGCGAAGGTGGCGGCCATTCCCGCCTTAGCG
  CTCTGTTCGCCCGGGAACGCAGGTTGGCCCTTGTAAGAAGAGCATGTGAAGAGGCTCTCGCCGGAGCCAG
  GTTGACTCATCTGTTCGACGCCGTTGCTCCCGGCGCCACTGAAAGGTTGTTTTGCGGTGGAGTCTACTCT
  AGCTCAGGCGACGCCGTTGAGGCTCTGAAAGCTGATTGCGCAGCCGCTTTTACCGCCCACCCTCAATATA
  GGGCGATCCTCCAGAAAAGGAACGAACTTTATACCAGACTCAATAGGGCCATGCAGAGGTTGGGCAGGGG
  AGAGGAGGAAGCTTCCAGAGAGTCTCCAGAAGTCCCCAGACCGGCAGGAGCACGGGAGCCCGGTCCGAGC
  GGCGCGCTGTCAGATGCCCTCAAGCGGAAAGAGCAGTACCTTCGGCAGGTAGCTACCGAAGGACTGGCTA
  AGCTGCAGAGCTGCCTCGCTCAGCAGTCTGAGACACTCACAGAGACACTGTGTCTGAGAGTCTGGGGTGA
  TGTGGTGTACTGGGAGCTGGCCCGGATGCGAAACCACTTTCTGTACAGGAGAGCATTCGTTAGTGGCCCC
  TGGGAGGATCGCAGGGCTGGAGAAGGCGCAGCCTTCGAGAACAGTAAGTATATTAAAACTCATCTGTTCA
  CGCAAACACTGAGCTCCGAGCACCTCCACGCCCTCACCCATTCCCTCTACACGTTTATCACTGGCCCGCT
  GGCAGAGGAATCAGGCCTCTTTCCTCCACCATCAAACGTGGCTCTCGCCCGATGCTGCGACGCTGCTGGC
  ACACTGCCTCACCAGAAAGCCTTCCTTACATCCCTGATCTGGCCTGGCATAGAGCCAAGCGACTGGATTG
  AAACCTCCTTCAACTCTTTCTATTCCGTTCCCGGAGGCAGCCTGGCGTCCTCCCAGCAGATCCTCTGCCG
  CGCTCTCAGAGAAGCAGTCCTCACAGTGAGCCTCTATAACAAGACATGGGGCAGAAGCCTGATCCTGCGC
  AGGGCCGACGCCGTGAGTCCCGGGCAGGCCCTCCCCCCAGACGGGTTGTACCTGACTTATGACTCAGATC
  GACCACTGATCCTCCTGTATAAGGGACGGGGGTGGGTGTTCAAGGATCTGTACGCACTGCTGTATCTTCA
  CTTGCAGATGAGAGATGACTCAGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101085 representing YP_001129509
  Red=Cloning sites Green=Tags
  
  MSGLLAAAYSQVYALAVELSVCARLDPRSLDVAAVVRNAGLLAELEAILLPRLRRQNDRACSALSLELVH
  LLENSREASAALLAPGRKGTRVPPLRTPSVAYSVEFYGGHKVDVSLCLINDIEILMKRINSVFYCMSHTM
  GLESLERALDLLGRFRGVSPIPDPRLYITSVPCWRCVGELMVLPNHGNPSTAEGTHVSCNHLAVPVNPEP
  VSGLFENEVRQAGLGHLLEAEEKARPGGPEEGAVPGPGRPEAEGATRALDTYNVFSTVPPEVAELSELLY
  WNSGGHAIGATGQGEGGGHSRLSALFARERRLALVRRACEEALAGARLTHLFDAVAPGATERLFCGGVYS
  SSGDAVEALKADCAAAFTAHPQYRAILQKRNELYTRLNRAMQRLGRGEEEASRESPEVPRPAGAREPGPS
  GALSDALKRKEQYLRQVATEGLAKLQSCLAQQSETLTETLCLRVWGDVVYWELARMRNHFLYRRAFVSGP
  WEDRRAGEGAAFENSKYIKTHLFTQTLSSEHLHALTHSLYTFITGPLAEESGLFPPPSNVALARCCDAAG
  TLPHQKAFLTSLIWPGIEPSDWIETSFNSFYSVPGGSLASSQQILCRALREAVLTVSLYNKTWGRSLILR
  RADAVSPGQALPPDGLYLTYDSDRPLILLYKGRGWVFKDLYALLYLHLQMRDDSA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 2055 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009334.1, YP_001129509
• RefSeq ORF: 2055
• Locus ID: 5176189
• MW: 74.8 kDa