hypothetical protein (NC_009334) Virus Tagged ORF Clone

CAT#: VC101080

Myc-DDK-tagged ORF clone of viral ORF for LF2 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129504

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: hypothetical protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101080 represents NCBI reference of YP_001129504 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGAAGCTTATCCTGGGGGCGCCCACGCTGCTCTGGCATCCCGGCGCTCTAGCTTTCGGAACTCTC
  TGAGACGCCTCCGCCCCACAGAAAAACCAGATACATCTTTTATGCGAGGTGTGTGGAAGTACGAAATCTT
  TCCCTCCTACGTAAGAGTCACCAATAAGCAGGTGCTGCAACTGGACGCCCAGTGTCAGGAGTTGCCACCA
  TGCCCTAGCGTTGGCCAGATCCTCTCCTTCAAACTCCCAAGCTTTAGCTTCAATACCACCACGTACGGGT
  CCAGGTACTTTACGGTTGCTTTCCTGTTTTTTGGGGCCGAGGATAACGAAGTGTTTCTGAAGCCGTTTTT
  CGTCATGCACTCCGATCAGGACATTGTTCTGTCCGTGCTCAACCCCAGGTCTCTCTTTATCGAGAAAGGT
  AAGTTTACTTGGTATATAGTGCCAATTCGATTGGTGAAAAATCCCTATCTGTACCTGCAGATACTGCCTG
  GCCAGTCCGATATTCAATTGACAAGAAGCTGCACCCAAAGTGGAGACAAGCTGAACACTTCTGAACCCCA
  GATCTTTTTGTCTGGATCCCCAGTGACTAGCCAGGACGAATGCCTGCCATATCTGCTGGCGCAGCACACT
  CCACCCTTTTTGAAGTCCTACGCTAGGATCCACACCTTCCCTGGGAAAGTTTGTCCGGTGAATGCTATCA
  GACGCGGAAAAGGGTACGTCAGAGTGTCCGTCGACACCCCAGATCTGAAACGGGAGGGGCCACTTAATGT
  GAAGGTAGGCATGACCCTCCTGGACGACGTGATCATCGCTTTCCGATATAACCCATATCCTAAGAGCCAC
  TGGAGATGGGACGGGGAATCTACCGATATACGATATTTTGGCAGCCCTGTTATTATTCCACCTAACTTCA
  TAACAGAATTGGAGTATAATAATACCTACGAGGCTCCTCTGTCCTCAAAAATTACAGCGGTTGTCGTGAG
  CCACTCCAGCAACCCTGTCTTTTACGTCTACCCACAAGAATGGAAACCCGGTCAGACCCTCAAACTGACA
  GTCAGAAATATCTCTAATAATCCCATCACAATAGTCACCGGCCAGAGTATGGCGCAGGCCTTTTTCATCT
  ACGCAGGTGACCCAAGCATCAGTACCATCATGCGACGCTACATCCAGCGCCAGGGCTGTGCGTTGACATT
  GCCAGGCAATATCGTAGTTGAGAGTAGCAGCCTGCCCACTTTCGAGAGAATCAACAAGACGTTCAATGGC
  AACATTGTCGCCTCTGAAGGAACACTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101080 representing YP_001129504
  Red=Cloning sites Green=Tags
  
  MAEAYPGGAHAALASRRSSFRNSLRRLRPTEKPDTSFMRGVWKYEIFPSYVRVTNKQVLQLDAQCQELPP
  CPSVGQILSFKLPSFSFNTTTYGSRYFTVAFLFFGAEDNEVFLKPFFVMHSDQDIVLSVLNPRSLFIEKG
  KFTWYIVPIRLVKNPYLYLQILPGQSDIQLTRSCTQSGDKLNTSEPQIFLSGSPVTSQDECLPYLLAQHT
  PPFLKSYARIHTFPGKVCPVNAIRRGKGYVRVSVDTPDLKREGPLNVKVGMTLLDDVIIAFRYNPYPKSH
  WRWDGESTDIRYFGSPVIIPPNFITELEYNNTYEAPLSSKITAVVVSHSSNPVFYVYPQEWKPGQTLKLT
  VRNISNNPITIVTGQSMAQAFFIYAGDPSISTIMRRYIQRQGCALTLPGNIVVESSSLPTFERINKTFNG
  NIVASEGTL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 1287 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009334.1, YP_001129504
• RefSeq ORF: 1287
• Locus ID: 5176211
• MW: 48.4 kDa