BBRF2 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101054

Myc-DDK-tagged ORF clone of viral ORF for BBRF2 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129477

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BBRF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101054 represents NCBI reference of YP_001129477 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGTGGCAAGCACCATCAGCCCGGAGGCACAAGATCCCTTACCATGCAGAAAGTATCCCTGCGCG
  TGACCCCTCGGCTGGTGCTGGAGGTGAATAGACACAATGCAATTTGCGTGGCCACTAATGTGCCTGAGTT
  TTACAATGCTAGAGGGGATCTTAATATCCGGGATCTTCGGGCACACGTCAAGGCCAGGATGATCTCCAGT
  CAATTTTGCGGATACGTGCTGGTGTCACTGCTTGACTCTGAGGATCAGGTCGATCATCTGAACATTTTCC
  CTCACGTGTTCTCCGAGCGGATGATCTTGTATAAGCCTAATAACGTGAATCTCATGGAGATGTGCGCCCT
  GCTGTCTATGATTGAAAACGCCAAGTCCCCATCAATCGGACTGTGCCGGGAGGTACTCGGCCGCTTGACC
  TTGCTGCACTCTAAATGCAATAACCTGGACAGTCTGTTCCTTTACAATGGTGCCCGCACTCTTCTTTCTA
  CACTGGTCAAATACCATGATCTGGAGGAGGGAGCAGCCACTCCTGGTCCGTGGAATGAAGGTCTCAGCCT
  TTTTAAACTGCACAAAGAACTTAAGCGCGCACCAAGTGAGGCGCGCGATTTGATGCAGAGTTTGTTCCTG
  ACCAGCGGCAAGATGGGGTGCCTGGCCAGGAGCCCTAAGGACTATTGTGCCGATCTCAACAAGGAAGAGG
  ATGCGAACAGCGGATTCACGTTCAATCTGTTTTACCAAGATAGTCTGCTTACCAAGCATTTCCAGTGTCA
  GACTGTGTTGCAGACACTGAGAAGGAAGTGCTTGGGAAGCGACACTGTGAGCAAGATAATTCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101054 representing YP_001129477
  Red=Cloning sites Green=Tags
  
  MASGKHHQPGGTRSLTMQKVSLRVTPRLVLEVNRHNAICVATNVPEFYNARGDLNIRDLRAHVKARMISS
  QFCGYVLVSLLDSEDQVDHLNIFPHVFSERMILYKPNNVNLMEMCALLSMIENAKSPSIGLCREVLGRLT
  LLHSKCNNLDSLFLYNGARTLLSTLVKYHDLEEGAATPGPWNEGLSLFKLHKELKRAPSEARDLMQSLFL
  TSGKMGCLARSPKDYCADLNKEEDANSGFTFNLFYQDSLLTKHFQCQTVLQTLRRKCLGSDTVSKIIP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 834 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009334.1, YP_001129477
• RefSeq ORF: 834
• Locus ID: 5176152
• MW: 31.3 kDa