BGLF1 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101063

Myc-DDK-tagged ORF clone of viral ORF for BGLF1 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129487

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BGLF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101063 represents NCBI reference of YP_001129487 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGTGCACATCGATAATCAAGTGTTGAGTGGCTTGGGCACTCCCTTGCTTGTTCATCTGTTTGTGC
  CAGATACAGTGATGGCCGAACTGTGCCCTAATCGGGTCCCTAATTGTGAGGGTGCTTGGTGCCAGACACT
  TTTTTCCGACAGGACCGGCCTGACACGAGTGTGCCGCGTCTTTGCTGCGCGCGGAATGCTGCCCGGTCGA
  CCAAGCCACAGAGGGACTTTCACTAGTGTGCCTGTGTACTGCGAGGAAGGCCTCCCAGAGCTGTACAACC
  CCTTTCACGTGGCTGCTCTTAGATTTTACGACGAGGGAGGACTGGTTGGCGAACTGCAGATATACTACCT
  GAGTCTTTTTGAGGGAGCTAAACGCGCCTTGACTGACGGTCACCTTATACGAGAGGCAAGCGGGGTTCAG
  GAGTCCGCCGCGGCCATGCAACCCATCCCGATTGACCCCGGACCTCCCGGTGGGGCCGGGATCGAGCACA
  TGCCAATGGCTGCAGCACAAGTGGAGCACCCAAAAACTTACGACCTGAAGCAGATTTTGCTGGAAATAAC
  TCAGGAGGAGAATCGAGGTGAGCAGCGCCTGGGCCACGCGGGTTCACCTGCATTGTGCTTGGGATTGAGG
  CTCCGAGCGGGCGCAGAGACCAAGGCTGCAGCTGAGACATCTGTCTCAAAGCACCATCCTGCTCTGGAAA
  ATCCCTCTAATATCCGCGGGAGCGCAGGCGGCGAAGGTGGTGGAGGCCGGGCAGGCACTGGTGGAACAGT
  TGGAGTCGGTTCTGGCGCACTCTCCAGAGTGCCAGTCAGCTTTTCCAAGACTAGGAGAGCTATACGAGAG
  AGTAGAGCGCTGGTTCGGGGTATTGCCCACATCTTTAGCCCACATGCTCTCTACGTGGTCACATATCCTG
  AGCTGTCCGCCCAGGGCAGACTGCATCGGATGACCGCTGTGACCCACGCTTCCCCCGCTACAGATCTTGC
  AGAAGTCAGTATCCTGGGCGCTCCTGAACGGGAGTTTCGCTTCCTTATAAGCGTTGCCCTGAGAATTAGC
  GCCTCTTTCCGCGAGAAGCTGGCAATGCAAGCGTGGACAGCCCAGCAAGAGATTCCAGTGGTGATTCCTA
  CCTCTTATTCCCGCATTTATAAAAATTCTGATCTGATTCGCGAAGCATTTTTCACTGTACAAACAAGAGT
  CAGTTGGGAGAGTTGTTGGGTGAAAGCAATTAGCAACGCCCCAAAGACACCCGATGCATGCCTGTGGATT
  GACTCCCATCCACTGTATGAGGAAGGCGCCTCCGCTTGGGGCAAGGTGATTGATAGCAGACCTCCTGGTG
  GTCTGGTGGGGGCAGCCAGTCAACTGGTCGCCCTCGGAACAGACGGTCATTGCGTGCACCTGGCTACCAC
  TTCTGACGGTCAGGCCTTCTTGGTTCTCCCAGGCGGCTTCGTAATTAAGGGACAGCTGGCGCTGACTCCC
  GAAGAAAGGGGCTACATTCTTGCACGGCATGGAATTAGGCGAGAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101063 representing YP_001129487
  Red=Cloning sites Green=Tags
  
  MDVHIDNQVLSGLGTPLLVHLFVPDTVMAELCPNRVPNCEGAWCQTLFSDRTGLTRVCRVFAARGMLPGR
  PSHRGTFTSVPVYCEEGLPELYNPFHVAALRFYDEGGLVGELQIYYLSLFEGAKRALTDGHLIREASGVQ
  ESAAAMQPIPIDPGPPGGAGIEHMPMAAAQVEHPKTYDLKQILLEITQEENRGEQRLGHAGSPALCLGLR
  LRAGAETKAAAETSVSKHHPALENPSNIRGSAGGEGGGGRAGTGGTVGVGSGALSRVPVSFSKTRRAIRE
  SRALVRGIAHIFSPHALYVVTYPELSAQGRLHRMTAVTHASPATDLAEVSILGAPEREFRFLISVALRIS
  ASFREKLAMQAWTAQQEIPVVIPTSYSRIYKNSDLIREAFFTVQTRVSWESCWVKAISNAPKTPDACLWI
  DSHPLYEEGASAWGKVIDSRPPGGLVGAASQLVALGTDGHCVHLATTSDGQAFLVLPGGFVIKGQLALTP
  EERGYILARHGIRREQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 1518 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009334.1, YP_001129487
• RefSeq ORF: 1518
• Locus ID: 5176151
• MW: 54.4 kDa