BALF5 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101083

Myc-DDK-tagged ORF clone of viral ORF for BALF5 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129507

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BALF5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101083 represents NCBI reference of YP_001129507 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGGGGGCCTGTTCTATAACCCCTTTCTGCGGCCCAACAAGGGTCTGCTCAAGAAACCCGACAAGG
  AATATCTGCGGTTGATTCCTAAGTGTTTTCAAACCCCCGGTGCAGCCGGGGTCGTGGACGTCAGGGGGCC
  ACAACCGCCCCTGTGTTTTTACCAGGATTCATTGACCGTGGTGGGCGGAGACGAGGATGGCAAGGGTATG
  TGGTGGAGGCAGCGCGCACAGGAAGGCACAGCGAGACCCGAAGCCGATACCCATGGGTCACCACTCGACT
  TCCACGTGTATGATATCCTTGAGACCGTTTATACCCACGAAAAATGTGCGGTGATTCCTAGCGACAAACA
  AGGCTACGTCGTCCCATGTGGCATCGTAATTAAGCTCCTCGGTAGACGGAAAGCCGACGGCGCTAGCGTT
  TGCGTAAACGTCTTCGGTCAGCAGGCTTATTTCTACGCCAGCGCCCCGCAGGGTCTGGACGTTGAATTCG
  CTGTGCTCAGCGCACTCAAGGCCAGTACCTTCGACAGACGCACACCATGTCGGGTGAGCGTTGAGAAGGT
  GACAAGGAGAAGTATCATGGGGTACGGGAACCATGCCGGGGATTACCATAAGATTACCCTCAGCCACCCA
  AACAGTGTCTGCCATGTGGCTACCTGGCTCCAGGACAAGCACGGCTGCCGCATCTTTGAGGCTAACGTCG
  ACGCCACGAGGAGGTTTGTACTCGACAACGATTTTGTCACTTTCGGATGGTATAGCTGCAGGAGAGCTAT
  TCCACGGCTCCAACATAGAGATAGCTACGCAGAACTGGAATACGACTGTGAGGTGGGCGATCTGAGTGTG
  CGGAGAGAAGATTCCTCTTGGCCATCCTACCAAGCACTGGCGTTCGATATTGAGTGCCTCGGAGAAGAAG
  GTTTTCCTACTGCGACGAACGAGGCAGACCTGATCTTGCAGATTTCATGCGTGCTGTGGTCAACAGGAGA
  AGAGGCCGGGCGGTACAGACGAATCCTCCTGACCCTGGGAACCTGCGAAGACATCGAGGGCGTAGAGGTG
  TACGAATTTCCCAGCGAGCTTGACATGCTCTATGCCTTCTTTCAGCTTATTCGCGACCTCTCTGTGGAGA
  TCGTGACCGGCTATAATGTTGCCAATTTCGACTGGCCCTACATCCTGGACCGCGCCCGACACATTTACTC
  CATTAATCCAGCTAGCCTGGGGAAAATAAGAGCTGGTGGCGTCTGTGAGGTGAGGAGGCCCCACGACGCT
  GGAAAAGGATTCCTTAGAGCCAATACCAAGGTGCGGATAACTGGTCTGATCCCAATTGACATGTATGCTG
  TGTGTAGGGACAAACTGTCTCTGAGTGACTATAAACTCGACACCGTCGCCAGGCATCTGCTGGGAGCTAA
  AAAAGAGGATGTTCATTATAAGGAGATTCCCAGACTTTTCGCTGCTGGCCCCGAGGGCCGCAGGCGCCTT
  GGGATGTACTGCGTCCAGGATTCCGCCCTGGTCATGGATCTGCTTAATCATTTCGTGATTCACGTGGAGG
  TGGCCGAAATCGCCAAAATTGCTCATATCCCTTGCAGGCGAGTGTTGGACGATGGGCAGCAGATCCGAGT
  GTTCTCATGCCTGCTGGCCGCAGCGCAGAAGGAGAATTTCATCCTGCCAATGCCCAGTGCCAGCGACAGA
  GATGGTTACCAGGGGGCCACAGTGATTCAGCCACTGAGCGGATTTTACAATTCTCCGGTCCTGGTAGTCG
  ACTTCGCTAGTCTCTATCCCTCAATCATTCAGGCACACAACCTCTGTTATTCAACCATGATCACACCCGG
  CGAGGAACATAGGCTCGCTGGGCTTCGGCCCGGGGAGGACTACGAATCTTTCAGACTGACCGGTGGTGTA
  TACCATTTTGTCAAGAAGCACGTTCATGAGTCCTTCCTCGCCTCCTTGTTGACTTCATGGCTGGCAAAGA
  GAAAGGCGATAAAAAAACTCCTCGCGGCATGTGAGGATCCCAGGCAGCGGACTATTCTCGACAAGCAGCA
  GTTGGCGATCAAGTGCACCTGTAACGCCGTATATGGCTTCACTGGCGTGGCTAACGGGCTGTTTCCCTGC
  CTCAGCATTGCGGAGACGGTAACCCTCCAGGGAAGAACCATGCTGGAACGAGCCAAAGCATTTGTGGAGG
  CCCTGTCTCCGGCCAACCTGCAAGCTTTGGCTCCTTCACCTGATGCCTGGGCACCACTTAATCCTGAGGG
  TCAATTGAGGGTTATTTACGGGGATACAGACTCCTTGTTTATAGAATGTAGGGGATTTTCTGAATCTGAA
  ACACTGCGGTTTGCTGAGGCCTTGGCCGCCCACACAACAAGGTCCCTTTTCGTTGCTCCAATCAGTTTGG
  AAGCTGAAAAAACTTTCTCTTGTCTGATGCTGATCACTAAGAAACGGTACGTGGGCGTGCTGACCGACGG
  CAAAACTCTGATGAAAGGGGTGGAGTTGGTCAGGAAGACAGCTTGTAAATTCGTGCAGACTCGCTGTCGC
  AGAGTTCTGGATTTGGTTCTCGCAGACGCCAGGGTCAAGGAGGCTGCCTCCTTGTTGTCTCATAGGCCCT
  TCCAGGAGTCATTCACACAGGGCCTTCCTGTAGGTTTCCTGCCAGTGATTGATATCCTCAATCAGGCGTA
  CACAGACTTGCGGGAAGGGAGAGTGCCGATGGGCGAGCTTTGCTTTAGCACCGAGCTGAGTAGAAAGCTG
  TCAGCTTATAAATCAACACAGATGCCCCATCTGGCCGTGTACCAGAAATTCGTTGAACGGAATGAAGAAC
  TGCCCCAGATTCACGACAGAATCCAATATGTTTTCGTGGAACCCAAGGGCGGCGTGAAGGGAGCCAGAAA
  GACAGAGATGGCTGAAGATCCAGCCTATGCAGAACGGCACGGAGTCCCAGTGGCTGTTGATCACTACTTC
  GACAAACTGTTGCAGGGGGCTGCCAATATCCTGCAATGTCTGTTCGACAACAACAGCGGCGCTGCACTTA
  GCGTGCTGCAGAACTTCACTGCCAGGCCCCCCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101083 representing YP_001129507
  Red=Cloning sites Green=Tags
  
  MSGGLFYNPFLRPNKGLLKKPDKEYLRLIPKCFQTPGAAGVVDVRGPQPPLCFYQDSLTVVGGDEDGKGM
  WWRQRAQEGTARPEADTHGSPLDFHVYDILETVYTHEKCAVIPSDKQGYVVPCGIVIKLLGRRKADGASV
  CVNVFGQQAYFYASAPQGLDVEFAVLSALKASTFDRRTPCRVSVEKVTRRSIMGYGNHAGDYHKITLSHP
  NSVCHVATWLQDKHGCRIFEANVDATRRFVLDNDFVTFGWYSCRRAIPRLQHRDSYAELEYDCEVGDLSV
  RREDSSWPSYQALAFDIECLGEEGFPTATNEADLILQISCVLWSTGEEAGRYRRILLTLGTCEDIEGVEV
  YEFPSELDMLYAFFQLIRDLSVEIVTGYNVANFDWPYILDRARHIYSINPASLGKIRAGGVCEVRRPHDA
  GKGFLRANTKVRITGLIPIDMYAVCRDKLSLSDYKLDTVARHLLGAKKEDVHYKEIPRLFAAGPEGRRRL
  GMYCVQDSALVMDLLNHFVIHVEVAEIAKIAHIPCRRVLDDGQQIRVFSCLLAAAQKENFILPMPSASDR
  DGYQGATVIQPLSGFYNSPVLVVDFASLYPSIIQAHNLCYSTMITPGEEHRLAGLRPGEDYESFRLTGGV
  YHFVKKHVHESFLASLLTSWLAKRKAIKKLLAACEDPRQRTILDKQQLAIKCTCNAVYGFTGVANGLFPC
  LSIAETVTLQGRTMLERAKAFVEALSPANLQALAPSPDAWAPLNPEGQLRVIYGDTDSLFIECRGFSESE
  TLRFAEALAAHTTRSLFVAPISLEAEKTFSCLMLITKKRYVGVLTDGKTLMKGVELVRKTACKFVQTRCR
  RVLDLVLADARVKEAASLLSHRPFQESFTQGLPVGFLPVIDILNQAYTDLREGRVPMGELCFSTELSRKL
  SAYKSTQMPHLAVYQKFVERNEELPQIHDRIQYVFVEPKGGVKGARKTEMAEDPAYAERHGVPVAVDHYF
  DKLLQGAANILQCLFDNNSGAALSVLQNFTARPPF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 3045 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009334.1, YP_001129507
• RefSeq ORF: 3045
• Locus ID: 5176180
• MW: 113.4 kDa