Alkaline exonuclease (NC_009334) Virus Tagged ORF Clone

CAT#: VC101057

Myc-DDK-tagged ORF clone of viral ORF for BBLF1 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129480

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Alkaline exonuclease
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101057 represents NCBI reference of YP_001129480 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGCTTTGTGGAGCCTCTGTAGGAGGCGCGTGAATAGTATAGGGGATGTGGATGGGGGCATAATCA
  ACCTGTACAACGACTATGAGGAATTCAACCTCGAGACAACTAAGCTGATTGCCGCAGAAGAGGGCAGAGC
  ATGCGGGGAGACAAATGAGGGACTGGAGTATGACGAAGATAGTGAAAACGACGAACTCCTGTTTCTGCCT
  AATAAAAAACCTAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101057 representing YP_001129480
  Red=Cloning sites Green=Tags
  
  MGALWSLCRRRVNSIGDVDGGIINLYNDYEEFNLETTKLIAAEEGRACGETNEGLEYDEDSENDELLFLP
  NKKPN
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 225 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009334.1, YP_001129480
• RefSeq ORF: 225
• Locus ID: 5176158
• MW: 8.5 kDa