BMRF2 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101032

Myc-DDK-tagged ORF clone of viral ORF for BMRF2 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129455

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BMRF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101032 represents NCBI reference of YP_001129455 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTTCTTGCAAACAACATCTGTCCTTGGGGGCCTGCGTGTTCTGCTTGGGATTGTTGGCCTCAACTC
  CCTTTATCTGGTGTTTCGTGTTTGCCAACCTCCTCAGCCTGGAAATTTTCAGTCCCTGGCAGACCCACGT
  CTATCGCTTGGGCTTTCCTACAGCCTGCCTGATGGCCGTACTCTGGACCCTGGTCCCTGCTAAGCACGCC
  GTGAGGGCAGTTACGCCAGCCATAATGCTTAACATCGCGTCAGCCCTTATCTTCTTCTCCCTGAGGGTCT
  ACTCAACATCCACATGGGTTAGTGCACCCTGTCTGTTTCTGGCCAATCTCCCCCTGTTGTGCTTGTGGCC
  CCGCCTGGCAATCGAGATAGTTTACATTTGTCCTGCTATACACCAGCGCTTCTTCGAACTGGGTCTGCTG
  CTTGCTTGTACAATTTTTGCCTTGTCAGTGGTGAGCCGAGCACTGGAGGTCAGCGCAGTATTTATGAGCC
  CCTTTTTTATTTTTCTGGCCCTTGGGTCCGGCTCACTGGCCGGGGCACGCCGGAATCAGATCTACACTAG
  TGGATTGGAGCGACGCAGGTCCATCTTTTGTGCTCGAGGGGACCATAGCGTGGCATCCCTGAAAGAGACT
  CTTCACAAATGCCCCTGGGATCTCCTTGCTATTTCCGCCCTCACAGTGCTCGTGGTGTGCGTGATGATCG
  TGCTGCATGTCCACGCAGAAGTGTTCTTCGGCCTCTCACGGTATCTCCCGCTGTTCCTCTGTGGCGCCAT
  GGCAAGCGGGGGACTGTATCTCGGTCACTCCTCCATCATCGCCTGCGTTATGGCCACTCTGTGCACCTTG
  TCCAGCGTGGTTGTGTATTTTCTTCATGAAACGTTGGGTCCTCTGGGAAAGACCGTGCTTTTTATTTCAA
  TCTTCGTGTATTACTTCAGTGGCGTGGCCGCTCTCAGCGCCGCCATGAGGTACAAGCTCAAGAAATTCGT
  AAATGGACCTCTCGTGCATCTTAGAGTTGTATATATGTGTTGTTTCGTCTTCACCTTTTGCGAATACCTG
  CTGGTTACTTTTATTAAAAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101032 representing YP_001129455
  Red=Cloning sites Green=Tags
  
  MFSCKQHLSLGACVFCLGLLASTPFIWCFVFANLLSLEIFSPWQTHVYRLGFPTACLMAVLWTLVPAKHA
  VRAVTPAIMLNIASALIFFSLRVYSTSTWVSAPCLFLANLPLLCLWPRLAIEIVYICPAIHQRFFELGLL
  LACTIFALSVVSRALEVSAVFMSPFFIFLALGSGSLAGARRNQIYTSGLERRRSIFCARGDHSVASLKET
  LHKCPWDLLAISALTVLVVCVMIVLHVHAEVFFGLSRYLPLFLCGAMASGGLYLGHSSIIACVMATLCTL
  SSVVVYFLHETLGPLGKTVLFISIFVYYFSGVAALSAAMRYKLKKFVNGPLVHLRVVYMCCFVFTFCEYL
  LVTFIKS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 1071 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009334.1, YP_001129455
• RefSeq ORF: 1071
• Locus ID: 5176179
• MW: 39.5 kDa