BMRF1 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101031

Myc-DDK-tagged ORF clone of viral ORF for BMRF1 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129454

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BMRF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101031 represents NCBI reference of YP_001129454 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACCACACAAACTCTGAGATTTAAAACGAAAGCCCTTGCCGTTCTTTCTAAGTGCTACGACCATG
  CACAGACCCACCTGAAGGGTGGCGTCCTGCAGGTCAACCTGCTGAGCGTAAATTATGGAGGACCCAGGCT
  TGCAGCAGTGGCCAACGCCGGCACCGCCGGCCTCATATCATTCGAGGTGAGCCCTGATGCCGTTGCTGAA
  TGGCAGAACCACCAGTCTCCCGAGGAGGCACCCGCCGCCGTGTCCTTTCGGAATCTGGCATATGGCAGAA
  CATGCGTGCTCGGGAAGGAGCTGTTCGGATCAGCTGTGGAGCAGGCATCACTTCAGTTCTATAAGCGCCC
  CCAGGGCGGAAGCCGACCTGAGTTCGTGAAGCTCACAATGGAATACGATGATAAGGTGTCCAAGAGCCAC
  CACACATGTGCGCTGATGCCTTACATGCCGCCCGCCTCCGACAGGCTGCGGAATGAGCAGATGATTGGAC
  AAGTTCTGTTGATGCCCAAGACAGCGAGCTCACTGCAGAAATGGGCTCGCCAACAAGGTTCTGGCGGTGT
  CAAAGTGACCCTGAACCCTGACCTGTATGTTACCACTTACACGTCTGGCGAGGCCTGTCTGACACTGGAT
  TATAAGCCCCTGTCTGTTGGACCTTATGAGGCCTTCACTGGCCCTGTGGCAAAAGCCCAGGATGTGGGAG
  CTGTCGAAGCACATGTCGTTTGCTCCGTCGCAGCGGACAGCCTCGCCGCCGCGCTGTCACTGTGCAGGAT
  CCCTGCTGTTTCCGTGCCAATTCTGCGGTTTTATAGGAGTGGAATTATCGCCGTAGTCGCCGGTTTGCTC
  ACATCCGCGGGAGACCTGCCCCTGGACTTGAGTGTCATCCTCTTCAACCACGCAAGTGAGGAGGCTGCAG
  CCAGTACGGCCTCTGAACCCGAGGACAAGTCTCCCAGAGTTCAACCCCTGGGAACTGGACTGCAGCAGCG
  CCCCAGACACACCGTCTCCCCCTCTCCAAGCCCCCCCCCACCACCGAGGACACCCACTTGGGAAAGCCCG
  GCTAGACCAGAAACTCCCTCCCCAGCCATACCCAGCCACAGTTCTAATACAGCCCTGGAAAGGCCACTGG
  CCGTACAGCTGGCACGCAAGAGAACCTCCTCCGAGGCCAGACAGAAACAAAAACATCCAAAGAAAGTTAA
  GCAGGCCTTTAACCCCCTCATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101031 representing YP_001129454
  Red=Cloning sites Green=Tags
  
  METTQTLRFKTKALAVLSKCYDHAQTHLKGGVLQVNLLSVNYGGPRLAAVANAGTAGLISFEVSPDAVAE
  WQNHQSPEEAPAAVSFRNLAYGRTCVLGKELFGSAVEQASLQFYKRPQGGSRPEFVKLTMEYDDKVSKSH
  HTCALMPYMPPASDRLRNEQMIGQVLLMPKTASSLQKWARQQGSGGVKVTLNPDLYVTTYTSGEACLTLD
  YKPLSVGPYEAFTGPVAKAQDVGAVEAHVVCSVAADSLAAALSLCRIPAVSVPILRFYRSGIIAVVAGLL
  TSAGDLPLDLSVILFNHASEEAAASTASEPEDKSPRVQPLGTGLQQRPRHTVSPSPSPPPPPRTPTWESP
  ARPETPSPAIPSHSSNTALERPLAVQLARKRTSSEARQKQKHPKKVKQAFNPLI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 1212 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009334.1, YP_001129454
• RefSeq ORF: 1212
• Locus ID: 5176169
• MW: 43.4 kDa