BDLF4 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101065

Myc-DDK-tagged ORF clone of viral ORF for BDLF4 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129488

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BDLF4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101065 represents NCBI reference of YP_001129488 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGACCAGGGCCGCCTGTCTCTGCCCCGCGGCGAGGGTGGTACCGACGAACCTAATCCACGCCATT
  TGTGCAGCTACAGTAAACTGGAGTTCCACCTGCCTCTGCCTGAAAGTATGGCCTCAGTCTTTGCCTGCTG
  GGGTTGTGGCGAGTACCATGTGTGCGACGGAAGCAGCGAATGCACACTTATTGAGACGCATGAGGGGGTG
  GTGTGCGCGCTCACCGGGAACTATATGGGACCACACTTCCAGCCCGCGCTCAGGCCATGGACTGAAATCA
  GGCAAGATACGCAGGATCAGAGAGATAAGTGGGAACCAGAACAGGTTCAAGGGCTCGTCAAAACCGTGGT
  GAACCACTTGTACCACTATTTTCTGAACGAGAACGTGATTTCCGGCGTGAGCGAGGCGCTGTTCGATCAG
  GAGGGCGCGCTGAGGCCCCACATACCGGCACTGGTTTCCTTCGTGTTCCCCTGCTGTCTGATGCTCTTCC
  GAGGAGCATCAAGCGAGAAGGTCGTCGACGTGGTCCTCTCCCTGTATATTCATGTGATTATAAGCATCTA
  CAGCCAGAAAACAGTCTATGGCGCTCTTCTTTTTAAGTCCACAAGGAATAAACGCTACGACGCCGTCGCT
  AAGCGGATGCGCGAGCTCTGGATGTCTACTCTGACTACAAAGTGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101065 representing YP_001129488
  Red=Cloning sites Green=Tags
  
  MSDQGRLSLPRGEGGTDEPNPRHLCSYSKLEFHLPLPESMASVFACWGCGEYHVCDGSSECTLIETHEGV
  VCALTGNYMGPHFQPALRPWTEIRQDTQDQRDKWEPEQVQGLVKTVVNHLYHYFLNENVISGVSEALFDQ
  EGALRPHIPALVSFVFPCCLMLFRGASSEKVVDVVLSLYIHVIISIYSQKTVYGALLFKSTRNKRYDAVA
  KRMRELWMSTLTTKC
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 675 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009334.1, YP_001129488
• RefSeq ORF: 675
• Locus ID: 5176193
• MW: 25.4 kDa