BFRF1A (NC_009334) Virus Tagged ORF Clone

CAT#: VC101022

Myc-DDK-tagged ORF clone of viral ORF for BFRF1A [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129445

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BFRF1A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101022 represents NCBI reference of YP_001129445 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCACCTGGGCGGGAAGGTAAGGAGCAGCGGATTCCTTATTCCTGGTAGCGCACCCGTCAGTGGAA
  CCCCTCCACAAGGGCAGGGAGGGCTGGCCTCCTCCCGGGTGTACGAAGATAGTGGTGGTTCACTTCTGAG
  CTTCTCCCCAATGATACACCCGAATCTGGCTCCTCTTATAGCCCCCAGCGCGTATGCCAGGCTGAATCTT
  CTTAACCACTGCATCTTTCTTGAGGCGTTCAAGGAGGAGAACTCCGGGCAAGGACTCGGCGGCGTGCAAA
  GAGAGGTGCTCTTTCTCAAAAGGGACATCATCAAGCAAGTGATAGCTAGAATCGTCGCAGCAGACTCCAC
  GCTGGATCTGGTGAATCACGGGGAACCTGGGCGCGAAGCTCCAAGGAGAGCCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101022 representing YP_001129445
  Red=Cloning sites Green=Tags
  
  MTHLGGKVRSSGFLIPGSAPVSGTPPQGQGGLASSRVYEDSGGSLLSFSPMIHPNLAPLIAPSAYARLNL
  LNHCIFLEAFKEENSGQGLGGVQREVLFLKRDIIKQVIARIVAADSTLDLVNHGEPGREAPRRAQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 405 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009334.1, YP_001129445
• RefSeq ORF: 405
• Locus ID: 5176168
• MW: 14.3 kDa