K15 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101014

Myc-DDK-tagged ORF clone of viral ORF for K15 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129436

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: K15
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101014 represents NCBI reference of YP_001129436 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTCTCTTGAAATGGTGCCCATGGGTGCAGGACCACCTAGCCCTGGGGGCGACCCCGATGGCGATG
  ACGGCGGTAACAACAGTCAGTATCCCAGCGCAAGCGGGAGTAGCGGCAATACACCAACTCCACCTAACGA
  CGAGGAGCGCGAGTCCAACGAGGAGCCACCACCACCCTACGAAGATCCCTATTGGGGGAACGGGGATCGG
  CACTCTGACTACCAACCTCTGGGAACACAAGATCAGTCCCTCTATTTGGGGCTGCAGCATGATGGAAACG
  ACGGGTTGCCGCCACCCCCGTACAGCCCTCGCGATGACTCTTCACAACATATTTACGAAGAAGCAGGGCG
  AGGAAGTATGAACCCCGTCTGTCTCCCGGTTATTGTTGCTCCGTATCTTTTCTGGCTCGCCGCTATTGCT
  GCCTCTTGCTTCACCGCTAGCGTCAGTACTGTGGTCACAGCCACAGGACTGGCTCTTTCTCTGCTGCTGT
  TGGCCGCCGTGGCCAGTTCCTACGCAGCAGCCCAGCGGAAGCTGTTGACCCCTGTTACAGTTTTGACTGC
  TGTGGTTACTTTTTTCGCTATCTGCCTGACATGGCGAATCGAGGACCCTCCATTCAATTCACTGCTTTTT
  GCCTTGCTTGCTGCTGCCGGGGGCCTGCAGGGTATATACGTACTCGTTATGCTTGTCCTCTTGATACTGG
  CTTACCGAAGAAGGTGGAGGCGCCTCACCGTGTGTGGCGGCATCATGTTCCTGGCTTGTGTCCTGGTGCT
  GATCGTCGACGCTGTCCTCCAGTTGTCTCCGCTGCTCGGCGCCGTGACAGTAGTCAGCATGACCCTGCTC
  TTGCTCGCATTCGTCCTGTGGCTGAGCAGCCCAGGTGGACTGGGGACACTCGGGGCCGCCCTGCTGACCC
  TCGCGGCTGCACTCGCCCTCCTTGCCTCTCTGATCCTGGGAACACTCAATCTCACCACCATGTTCCTTCT
  GATGCTTCTTTGGACATTGGTTGTACTGCTTATCTGTTCTTCTTGTAGTTCATGCCCTCTTAGTAAAATC
  CTGCTCGCTCGCCTTTTTCTCTACGCTCTCGCACTTCTCCTCCTCGCATCAGCCTTGATTGCAGGGGGCA
  GCATTCTGCAGACCAACTTCAAAAGTTTGTCTTCTACAGAATTCATTCCAAACCTCTTTTGTATGTTGTT
  GCTCATTGTCGCAGGGATTCTGTTCATCCTGGCTATTCTGACTGAATGGGGGTCTGGGAACCGGACATAT
  GGTCCAGTCTTCATGTGCCTGGGGGGGCTCTTGACCATGGTTGCAGGCGCCGTTTGGTTGACTGTGATGA
  CAAACACACTGTTGTCAGCATGGATTCTGACCGCGGGGTTCTTGATTTTCCTGATAGGTTTCGCTCTGTT
  CGGTGTGATTAGATGCTGCCGGTATTGTTGCTATTACTGCCTCACCCTCGAGAGCGAAGAGCGCCCGCCA
  ACTCCCTACAGGAACACAGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101014 representing YP_001129436
  Red=Cloning sites Green=Tags
  
  MGSLEMVPMGAGPPSPGGDPDGDDGGNNSQYPSASGSSGNTPTPPNDEERESNEEPPPPYEDPYWGNGDR
  HSDYQPLGTQDQSLYLGLQHDGNDGLPPPPYSPRDDSSQHIYEEAGRGSMNPVCLPVIVAPYLFWLAAIA
  ASCFTASVSTVVTATGLALSLLLLAAVASSYAAAQRKLLTPVTVLTAVVTFFAICLTWRIEDPPFNSLLF
  ALLAAAGGLQGIYVLVMLVLLILAYRRRWRRLTVCGGIMFLACVLVLIVDAVLQLSPLLGAVTVVSMTLL
  LLAFVLWLSSPGGLGTLGAALLTLAAALALLASLILGTLNLTTMFLLMLLWTLVVLLICSSCSSCPLSKI
  LLARLFLYALALLLLASALIAGGSILQTNFKSLSSTEFIPNLFCMLLLIVAGILFILAILTEWGSGNRTY
  GPVFMCLGGLLTMVAGAVWLTVMTNTLLSAWILTAGFLIFLIGFALFGVIRCCRYCCYYCLTLESEERPP
  TPYRNTV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 1491 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009334.1, YP_001129436
• RefSeq ORF: 1491
• Locus ID: 5176182
• MW: 53.0 kDa