BGRF1/BDRF1 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101061

Myc-DDK-tagged ORF clone of viral ORF for BGRF1/BDRF1 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129485

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BGRF1/BDRF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101061 represents NCBI reference of YP_001129485 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTATGCATCTCAGAGGGGTAGGCTTACTGAAAATCTGCGGAATGCCCTCCAGCAGGATTCTACTA
  CACAGGGCTGCCTGGGTGCCGAAACTCCATCCATCATGTATACTGGAGCAAAATCTGACCGCTGGGCACA
  TCCTCTGGTGGGAACCATTCATGCCTCTAATTTGTACTGTCCAATGCTGAGAGCCTATTGCCGACACTAC
  GGTCCCAGGCCGGTGTTTGTAGCCTCCGACGAGTCTCTGCCTATGTTCGGCGCCTCCCCCGCACTCCACA
  CACCTGTCCAGGTACAGATGTGCCTCCTGCCAGAGCTTAGGGACACACTCCAGAGGCTCCTCCCGCCTCC
  CAACCTGGAAGATTCTGAGGCTCTCACTGAGTTCAAAACAAGTGTCTCCAGTGCCCGGGCGATACTGGAG
  GACCCTAATTTCCTCGAAATGAGGGAGTTTGTCACCTCACTCGCCAGCTTTCTGAGTGGGCAGTATAAAC
  ACAAACCCGCTCGGCTGGAAGCGTTCCAGAAACAAGTGGTCCTGCACAGCTTTTATTTCCTGATAAGCAT
  TAAATCACTGGAGATTACCGATACCATGTTTGATATTTTCCAAAGCGCATTTGGCCTGGAAGAAATGACA
  CTTGAGAAACTCCATATTTTTAAACAGAAGGCGAGCGTGTTTCTTATTCCTCGGCGACATGGCAAGACAT
  GGATCGTTGTAGCCATCATTTCCCTGATTCTTTCCAACCTTTCAAATGTCCAGATCGGATACGTTGCACA
  CCAGAAACATGTTGCTTCCGCTGTGTTCACAGAGATCATTGATACCCTGACCAAGAGCTTTGACAGTAAG
  AGAGTGGAGGTTAATAAAGAAACTAGTACCATCACCTTTAGGCACTCAGGGAAAATCAGCTCTACGGTTA
  TGTGCGCAACATGTTTCAATAAGAACTCTATTCGCGGGCAGACCTTTCACTTGCTTTTCGTTGACGAGGC
  CAATTTTATAAAAAAAGAGGCCCTGCCAGCCATCCTTGGATTCATGTTGCAGAAAGACGCCAAGATCATC
  TTCATTTCTTCAGTAAATTCCGCGGACCAAGCAACTTCTTTCCTGTACAAGCTGAAGGACGCCCAAGAAC
  GGCTCCTGAACGTCGTGTCATATGTATGTCAGGAACACCGGCAAGATTTCGACATGCAAGACAGTATGGT
  GTCCTGTCCATGCTTTCGGCTCCATATTCCATCTTACATAACCATGGATAGCAACATTCGAGCCACAACG
  AACCTCTTTCTTGACGGAGCTTTCAGCACAGAACTGATGGGCGATACCTCTAGTTTGAGTCAAGGATCAC
  TTTCACGAACCGTCCGGGATGATGCCATAAATCAACTCGAATTGTGCAGGGTGGACACTCTGAATCCTCG
  GGTTGCAGGAAGACTGGCTTCATCACTCTACGTGTACGTTGACCCCGCATATACAAATAACACATCAGCT
  TCCGGAACGGGGATCGCAGCAGTTACTCATGATCGGGCCGACCCTAATCGCGTGATCGTTCTTGGGCTGG
  AACATTTTTTTCTGAAGGATCTGACTGGTGATGCCGCCCTGCAGATAGCTACCTGCGTGGTGGCCCTTGT
  GAGTTCTATTGTAACCCTGCACCCGCACCTGGAAGAGGTGAAAGTTGCAGTGGAAGGTAACTCTAGCCAA
  GATAGCGCTGTAGCTATCGCTTCAATTATCGGCGAATCATGCCCTCTGCCTTGTGCCTTCGTCCATACAA
  AGGATAAAACATCAAGTCTGCAGTGGCCTATGTATCTGTTGACTAATGAGAAGTCTAAAGCTTTCGAAAG
  GCTCATTTATGCCGTTAACACCGCAAGTCTCTCTGCTAGTCAGGTTACCGTTTCCAACACGATTCAACTT
  AGTTTCGACCCTGTGCTGTACCTGATCAGCCAGATTAGAGCCATCAAGCCCATTCCGCTGCGGGACGGTA
  CTTACACCTACACCGGGAAACAAAGAAATCTGTCTGATGACGTTCTCGTCGCCCTCGTGATGGCCCACTT
  CCTCGCCACAACCCAGAAACATACCTTCAAAAAAGTGCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101061 representing YP_001129485
  Red=Cloning sites Green=Tags
  
  MLYASQRGRLTENLRNALQQDSTTQGCLGAETPSIMYTGAKSDRWAHPLVGTIHASNLYCPMLRAYCRHY
  GPRPVFVASDESLPMFGASPALHTPVQVQMCLLPELRDTLQRLLPPPNLEDSEALTEFKTSVSSARAILE
  DPNFLEMREFVTSLASFLSGQYKHKPARLEAFQKQVVLHSFYFLISIKSLEITDTMFDIFQSAFGLEEMT
  LEKLHIFKQKASVFLIPRRHGKTWIVVAIISLILSNLSNVQIGYVAHQKHVASAVFTEIIDTLTKSFDSK
  RVEVNKETSTITFRHSGKISSTVMCATCFNKNSIRGQTFHLLFVDEANFIKKEALPAILGFMLQKDAKII
  FISSVNSADQATSFLYKLKDAQERLLNVVSYVCQEHRQDFDMQDSMVSCPCFRLHIPSYITMDSNIRATT
  NLFLDGAFSTELMGDTSSLSQGSLSRTVRDDAINQLELCRVDTLNPRVAGRLASSLYVYVDPAYTNNTSA
  SGTGIAAVTHDRADPNRVIVLGLEHFFLKDLTGDAALQIATCVVALVSSIVTLHPHLEEVKVAVEGNSSQ
  DSAVAIASIIGESCPLPCAFVHTKDKTSSLQWPMYLLTNEKSKAFERLIYAVNTASLSASQVTVSNTIQL
  SFDPVLYLISQIRAIKPIPLRDGTYTYTGKQRNLSDDVLVALVMAHFLATTQKHTFKKVH
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 2070 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009334.1, YP_001129485
• RefSeq ORF: 2070
• Locus ID: 5176176
• MW: 76.7 kDa