LMP-2B (NC_009334) Virus Tagged ORF Clone

CAT#: VC101016

Myc-DDK-tagged ORF clone of viral ORF for LMP-2B [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129437

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LMP-2B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101016 represents NCBI reference of YP_001129437 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCCCGTGTGCCTGCCCGTCATCGTAGCACCTTACCTCTTTTGGCTGGCAGCAATTGCTGCAAGCT
  GCTTCACCGCTTCAGTTAGTACAGTCGTTACAGCGACTGGGCTCGCTCTTTCCCTGCTTCTGCTGGCCGC
  TGTTGCCAGCAGCTACGCTGCGGCCCAAAGAAAGCTCTTGACACCCGTAACTGTTCTTACCGCTGTGGTG
  ACCTTTTTCGCTATCTGCTTGACTTGGCGGATTGAGGATCCACCTTTCAACAGCCTGCTGTTTGCCCTGC
  TTGCGGCAGCAGGAGGACTCCAGGGTATTTACGTCCTCGTCATGCTGGTTCTGCTGATTCTGGCCTATAG
  GCGACGGTGGCGACGCCTTACTGTGTGCGGCGGCATTATGTTCTTGGCATGTGTCCTGGTTCTCATTGTC
  GATGCAGTGCTCCAGTTGAGTCCCCTTCTGGGGGCTGTTACGGTTGTGTCTATGACTCTGCTCCTCCTCG
  CATTTGTTCTCTGGCTTTCAAGCCCTGGCGGCCTCGGAACCCTCGGAGCTGCCCTGCTGACATTGGCAGC
  AGCCCTCGCACTCCTGGCAAGCCTCATTCTCGGCACACTGAACCTGACGACCATGTTCCTGCTGATGCTG
  CTCTGGACACTTGTGGTTCTGCTCATCTGTAGCTCCTGCAGCTCCTGTCCTCTCTCAAAAATTCTGCTGG
  CCCGGCTGTTTCTGTACGCCCTGGCTCTCCTGCTGTTGGCCTCAGCCCTGATAGCCGGCGGGAGCATTCT
  GCAGACTAATTTTAAAAGCCTTTCCAGCACCGAATTTATCCCAAACCTGTTCTGTATGCTCCTGCTCATC
  GTTGCCGGCATTCTGTTCATCCTTGCCATCCTCACCGAATGGGGGTCCGGTAACAGGACCTATGGGCCAG
  TGTTCATGTGTCTCGGAGGCCTCCTGACCATGGTGGCAGGTGCCGTCTGGCTGACAGTGATGACCAACAC
  ACTGCTGTCTGCTTGGATTCTGACGGCCGGGTTCCTGATTTTCCTTATTGGGTTCGCTCTGTTCGGCGTG
  ATTAGATGTTGCCGGTACTGTTGTTACTATTGCCTGACGCTGGAATCAGAGGAGAGACCACCTACCCCAT
  ATAGGAACACAGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101016 representing YP_001129437
  Red=Cloning sites Green=Tags
  
  MNPVCLPVIVAPYLFWLAAIAASCFTASVSTVVTATGLALSLLLLAAVASSYAAAQRKLLTPVTVLTAVV
  TFFAICLTWRIEDPPFNSLLFALLAAAGGLQGIYVLVMLVLLILAYRRRWRRLTVCGGIMFLACVLVLIV
  DAVLQLSPLLGAVTVVSMTLLLLAFVLWLSSPGGLGTLGAALLTLAAALALLASLILGTLNLTTMFLLML
  LWTLVVLLICSSCSSCPLSKILLARLFLYALALLLLASALIAGGSILQTNFKSLSSTEFIPNLFCMLLLI
  VAGILFILAILTEWGSGNRTYGPVFMCLGGLLTMVAGAVWLTVMTNTLLSAWILTAGFLIFLIGFALFGV
  IRCCRYCCYYCLTLESEERPPTPYRNTV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 1134 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009334.1, YP_001129437
• RefSeq ORF: 1134
• Locus ID: 5176195
• MW: 40.4 kDa