BSLF2/BMLF1 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101033

Myc-DDK-tagged ORF clone of viral ORF for BSLF2/BMLF1 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129456

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BSLF2/BMLF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101033 represents NCBI reference of YP_001129456 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCCTTCACAAAGACTCAGCCGAACTAGCAGCATTAGCTCAAATGAGGACCCTGCAGAGTCACATA
  TCTTGGAACTCGAGGCTGTATCCGACACAAATACCGATTGCGATATGGACCCCATGGAGGGCTCTGAAGA
  GCACAGTACAGATGGCGAAATCAGTTCTTCAGAAGAGGAAGACGAGGATCCCACACCTGCACACACCATT
  CCCGCCAGGCCCTCTTCTGTAGTGATTACGCCCACGAGCGCCTCTTTCGTCATCCCCCGCAAAAAATGGG
  ACTTGCAGGACAAAACAGTTACATTGCATCGATCTCCGCTTTGCAGAGATGAAGACGAGAAGGAAGAAAC
  CGGCAACAGTAGTTATACAAGAGGCCACAAGAGGCGCCGCGGTGAAGTGCACGGGTGCACCGACGAAAGT
  TATGGCAAACGAAGGCACTTGCCTCCAGGAGCCAGAGCCCCCAGAGCTCCTCGGGCACCAAGAGTCCCAA
  GAGCACCTCGCTCCCCAAGAGCTCCCAGAAGCAATAGGGCAACCCGCGGTCCCCGGTCTGAGAGTAGAGG
  TGCTGGTCGCAGCACGCGGAAACAAGCCCGCCAGGAACGCTCTCAAAGGCCTCTCCCCAATAAACCCTGG
  TTCGATATGAGCCTGGTCAAACCAGTAAGTAAGATCACATTCGTGACTCTCCCATCCCCCTTGGCTTCCC
  TTACCCTGGAACCCATTCAAGATCCATTTCTTCAGTCAATGTTGGCAGTGGCCGCTCATCCTGAAATAGG
  GGCATGGCAAAAAGTGCAACCTCGACATGAGCTCCGCCGGAGCTACAAAACTCTCCGAGAGTTCTTCACT
  AAGTCAACCAATAAAGACACCTGGCTGGATGCAAGGATGCAAGCAATCCAGAACGCCGGGCTCTGCACTC
  TCGTGGCTATGCTGGAAGAGACGATCTTTTGGCTGCAAGAAATCACATATCATGGTGATCTTCCACTGGC
  CCCTGCCGAGGATATTCTCCTGGCCTGCGCAATGTCACTTAGCAAGGTCATTCTTACCAAGCTGAAAGAG
  CTGGCACCCTGTTTTTTGCCCAATACTCGCGACTATAATTTCGTGAAGCAGCTCTTCTATATCACCTGTG
  CTACCGCCCGGCAGAATAAAGTTGTAGAGACGTTGAGCTCCTCCTATGTAAAGCAGCCCCTGTGCCTGTT
  GGCAGCGTACGCCGCGGTTGCTCCGGCTTACATCAATGCTAATTGCAGGCGCCGCCATGACGAAGTGGAA
  TTCTTGGGACACTACATCAAAAACTACAATCCTGGTACTCTGTCAAGCCTGCTTACTGAGGCCGTGGAGA
  CTCACACCAGGGATTGTAGATCTGCAAGTTGCTCCAGATTGGTGAGAGCAATACTCAGCCCAGGAACCGG
  GAGCTTGGGCCTCTTCTTCGTGCCTGGTCTCAACCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101033 representing YP_001129456
  Red=Cloning sites Green=Tags
  
  MVPSQRLSRTSSISSNEDPAESHILELEAVSDTNTDCDMDPMEGSEEHSTDGEISSSEEEDEDPTPAHTI
  PARPSSVVITPTSASFVIPRKKWDLQDKTVTLHRSPLCRDEDEKEETGNSSYTRGHKRRRGEVHGCTDES
  YGKRRHLPPGARAPRAPRAPRVPRAPRSPRAPRSNRATRGPRSESRGAGRSTRKQARQERSQRPLPNKPW
  FDMSLVKPVSKITFVTLPSPLASLTLEPIQDPFLQSMLAVAAHPEIGAWQKVQPRHELRRSYKTLREFFT
  KSTNKDTWLDARMQAIQNAGLCTLVAMLEETIFWLQEITYHGDLPLAPAEDILLACAMSLSKVILTKLKE
  LAPCFLPNTRDYNFVKQLFYITCATARQNKVVETLSSSYVKQPLCLLAAYAAVAPAYINANCRRRHDEVE
  FLGHYIKNYNPGTLSSLLTEAVETHTRDCRSASCSRLVRAILSPGTGSLGLFFVPGLNQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 1437 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009334.1, YP_001129456
• RefSeq ORF: 1437
• Locus ID: 5176227
• MW: 53.5 kDa