BNLF2a (NC_009334) Virus Tagged ORF Clone

CAT#: VC101090

Myc-DDK-tagged ORF clone of viral ORF for BNLF2a [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129514

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BNLF2a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101090 represents NCBI reference of YP_001129514 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTCATGTTCTGGAGCGGGCGCTTCTGGAGCAACAGTCCAGCGCTTGTGGCCTGCCCGGAAGTAGCA
  CCGAAACCCGCCCTTCCCACCCATGTCCTGAGGATCCAGATGTTAGCCGACTCCGATTGCTGCTGGTCGT
  TCTCTGTGTACTGTTCGGATTGCTGTGTCTGCTGCTGATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101090 representing YP_001129514
  Red=Cloning sites Green=Tags
  
  MVHVLERALLEQQSSACGLPGSSTETRPSHPCPEDPDVSRLRLLLVVLCVLFGLLCLLLI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 180 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009334.1, YP_001129514
• RefSeq ORF: 180
• Locus ID: 5176159
• MW: 6.5 kDa