BcLF1 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101070

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for BcLF1 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129493


  View other clones from "Virus" (71)

Reconstitution Protocol

USD 2,190.00

6 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Symbol BcLF1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101070 represents NCBI reference of YP_001129493 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTCTAACGAGGGGGTGGAAAATCGGCCTTTCCCCTATCTCACTGTCGACGCGGATCTCCTTTCCA
ACTTGCGCCAGAGCGCAGCTGAAGGCCTCTTTCATTCTTTTGACCTGCTGGTGGGAAAAGATGCAAGGGA
GGCTGGAATCAAATTTGAAGTTTTGCTCGGGGTTTACACAAATGCGATTCAGTATGTGAGATTTCTTGAG
ACCGCTCTGGCTGTGTCTTGCGTTAATACTGAGTTTAAAGATCTGAGCAGAATGACCGACGGCAAGATAC
AGTTTAGAATTAGCGTGCCTACCATAGCACACGGCGATGGCAGAAGGCCCTCAAAGCAGCGCACCTTCAT
CGTGGTGAAGAACTGTCACAAACACCATATTTCCACCGAGATGGAACTGAGCATGCTGGATCTCGAGATA
CTGCACTCCATTCCAGAAACCCCCGTCGAGTATGCCGAATACGTTGGAGCAGTAAAAACAGTTGCTAGCG
CCCTCCAGTTCGGTGTTGACGCACTCGAGAGAGGACTTATAAACACAGTGCTGTCAGTGAAGCTCAGACA
CGCACCACCCATGTTTATATTGCAGACACTGGCGGATCCGACTTTCACGGAGAGAGGATTTAGCAAGACC
GTGAAGAGCGATCTTATCGCCATGTTCAAGCGCCACCTCCTTGAGCACAGCTTCTTCCTGGATAGAGCTG
AGAATATGGGGTCTGGGTTCTCACAATACGTACGATCTCGCCTGAGCGAGATGGTTGCAGCCGTGTCAGG
TGAAAGCGTGCTGAAGGGCGTCTCTACTTACACAACTGCCAAGGGTGGCGAACCCGTCGGAGGGGTGTTT
ATCGTAACCGACAATGTGCTGCGCCAGCTGCTGACCTTTCTTGGTGAAGAGGCTGATAACCAGATCATGG
GTCCCTCCTCCTATGCCAGCTTTGTTGTTAGGGGCGAGAACCTCGTAACTGCTGTGTCCTATGGTAGGGT
GATGCGAACATTCGAACACTTCATGGCCAGGATTGTCGATTCTCCCGAGAAAGCCGGATCAACCAAGTCC
GACTTGCCTGCTGTTGCCGCAGGCGTTGAGGATCAGCCCAGGGTGCCTATATCTGCCGCCGTGATTAAAC
TGGGGAATCATGCTGTTGCTGTGGAGTCCCTGCAAAAGATGTATAATGACACTCAATCCCCCTACCCACT
GAACAGGCGAATGCAGTACAGCTATTACTTTCCTGTCGGACTGTTCATGCCTAACCCTAAGTACACTACA
AGTGCGGCTATAAAGATGCTTGACAATCCCACCCAGCAACTGCCTGTGGAGGCATGGATCGTTAATAAAA
ATAACCTGCTGCTCGCCTTCAATCTCCAGAACGCACTCAAAGTCTTGTGTCACCCTCGCCTCCATACACC
TGCCCACACCCTTAATTCCCTGAATGCTGCACCCGCTCCGAGAGACCGAAGGGAAACTTACAGTCTGCAA
CATAGGAGACCCAACCACATGAACGTCCTTGTCATCGTGGATGAGTTCTATGACAACAAGTACGCTGCAC
CAGTTACAGACATTGCACTTAAGTGTGGGCTTCCAACAGAGGATTTCCTGCACCCCTCTAATTACGACCT
CCTTCGCCTGGAGCTCCATCCTCTTTACGATATATACATCGGACGGGACGCCGGCGAAAGGGCTCGGCAC
AGAGCTGTGCATCGCCTGATGGTGGGTAATTTGCCCACACCCCTGGCCCCAGCGGCCTTCCAGGAGGCCA
GAGGACAGCAATTTGAGACCGCCACCAGCCTGGCACACGTCGTAGACCAGGCTGTAATCGAAACGGTGCA
GGATACAGCATATGATACCGCCTACCCGGCCTTTTTTTATGTTGTGGAGGCCATGATCCACGGTTTCGAG
GAAAAGTTCGTGATGAACGTTCCGCTGGTCTCTCTTTGCATCAACACATACTGGGAAAGAGCGGGACGGC
TTGCCTTCGTAAATAGCTTCAGCATGATTAAGTTCATCTGCCGACACCTCGGCAACAACGCTATATCCAA
GGAGGCCTATTCCATGTACAGGAAGATTTACGGTGAACTGATTGCCCTCGAGCAAGCCCTCATGCGACTC
GCCGGCTCAGACGTTGTTGGCGACGAGTCCGTTGGCCAATACGTTTGTGCACTGCTTGATCCCAACCTGC
TTCCACCCGTGGCTTATACGGACATCTTTACCCACCTGCTTACTGTGAGTGATCGCGCCCCCCAAATTAT
CATAGGAAATGAGGTTTACGCCGACACACTGGCCGCGCCCCAGTTCATCGAAAGGGTTGGGAACATGGAT
GAGATGGCTGCTCAGTTTGTGGCGCTCTACGGCTACCGCGTCAATGGAGATCATGACCATGACTTCCGAC
TGCATCTTGGCCCTTACGTGGACGAAGGACACGCGGATGTGTTGGAAAAAATATTTTACTACGTCTTCCT
GCCCACATGTACCAACGCGCATATGTGCGGGCTGGGTGTAGATTTCCAGCACGTGGCTCAAACTCTCGCA
TACAATGGCCCGGCTTTTAGCCACCATTTCACTAGGGATGAGGATATTCTGGATAACCTTGAAAACGGCA
CTTTGAGAGATCTCTTGGAGATCAGTGATCTTCGACCGACCGTAGGCATGATACGCGATCTGAGTGCCTC
CTTTATGACTTGTCCAACCTTTACTAGAACCGTGCGGGTGAGCGTGGATAATGATGTAACCCAGCAGCTG
GCTCCTAACCCTGCTGATAAGCGCACGGAGCAGACAGTCCTGGTTAACGGGCTGGTGGCCTTCGCCTTCA
GCGAGCGAACCCGCGCAGTGACCCAGTGTCTGTTTCACGCGATCCCATTCCACATGTTCTACGGAGACCC
TCGCGTCGCCGCAACCATGCACCAGGATGTGGCAACTTTTGTGATGCGGAATCCGCAGCAGCGGGCTGTG
GAAGCATTCAACCGACCAGAGCAACTTTTCGCCGAGTACAGGGAGTGGCACAGGTCCCCAATGGGCAAGT
ACGCCGCTGAGTGTCTTCCCTCCTTGGTGAGCATCTCAGGGATGACCGCAATGCATATCAAGATGAGCCC
CATGGCGTACATTGCCCAGGCGAAATTGAAGATCCACCCCGGAGTGGCTATGACGGTAGTTAGAACTGAT
GAGATCCTGAGTGAAAACATACTGTTCTCCTCCCGGGCCTCAACAAGTATGTTCATCGGGACCCCAAACG
TGAGTCGCCGGGAGGCAAGAGTTGACGCAGTAACGTTTGAGGTACACCACGAAATGGCAAGCATAGACAC
TGGGCTGTCCTATTCTTCCACAATGACACCTGCGCGGGTAGCCGCCATCACAACTGACATGGGCATTCAT
ACACAGGATTTCTTTTCTGTTTTTCCCGCCGAGGCCTTCGGGAATCAGCAGGTCAATGACTATATTAAAG
CAAAAGTAGGAGCGCAGCGAAACGGTACTCTGCTGCGGGACCCCCGGACTTACCTCGCCGGCATGACCAA
CGTTAACGGAGCACCTGGACTGTGTCATGGACAGCAGGCCACTTGCGAAATCATTGTCACCCCAGTCACT
GCAGATGTCGCGTACTTTCAAAAGTCCAACTCCCCACGCGGCAGAGCTGCTTGCGTTGTTTCCTGCGAAA
ACTACAATCAAGAAGTGGCCGAAGGGCTGATTTATGATCACAGCCGACCCGATGCTGCCTATGAATATCG
GAGTACCGTAAACCCTTGGGCATCTCAGCTGGGGAGCCTGGGAGATATCATGTATAACTCAAGCTATAGG
CAGACAGCTGTGCCAGGTCTGTACTCTCCATGTCGCGCTTTCTTTAACAAGGAGGAACTGCTGCGGAATA
ACCGGGGACTCTACAATATGGTCAACGAATACTCCCAGAGGCTGGGCGGGCACCCTGCTACTAGTAATAC
AGAGGTCCAATTTGTAGTGATCGCCGGGACCGACGTGTTCTTGGAACAACCCTGCTCATTTCTGCAGGAG
GCATTCCCCGCCCTGTCCGCAAGCAGTCGGGCTCTGATAGACGAGTTCATGTCCGTGAAACAGACCCACG
CCCCCATTCACTATGGACACTATATCATTGAGGAGGTAGCACCCGTGAGACGAATTCTCAAGTTCGGCAA
CAAGGTTGTGTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101070 representing YP_001129493
Red=Cloning sites Green=Tags

MASNEGVENRPFPYLTVDADLLSNLRQSAAEGLFHSFDLLVGKDAREAGIKFEVLLGVYTNAIQYVRFLE
TALAVSCVNTEFKDLSRMTDGKIQFRISVPTIAHGDGRRPSKQRTFIVVKNCHKHHISTEMELSMLDLEI
LHSIPETPVEYAEYVGAVKTVASALQFGVDALERGLINTVLSVKLRHAPPMFILQTLADPTFTERGFSKT
VKSDLIAMFKRHLLEHSFFLDRAENMGSGFSQYVRSRLSEMVAAVSGESVLKGVSTYTTAKGGEPVGGVF
IVTDNVLRQLLTFLGEEADNQIMGPSSYASFVVRGENLVTAVSYGRVMRTFEHFMARIVDSPEKAGSTKS
DLPAVAAGVEDQPRVPISAAVIKLGNHAVAVESLQKMYNDTQSPYPLNRRMQYSYYFPVGLFMPNPKYTT
SAAIKMLDNPTQQLPVEAWIVNKNNLLLAFNLQNALKVLCHPRLHTPAHTLNSLNAAPAPRDRRETYSLQ
HRRPNHMNVLVIVDEFYDNKYAAPVTDIALKCGLPTEDFLHPSNYDLLRLELHPLYDIYIGRDAGERARH
RAVHRLMVGNLPTPLAPAAFQEARGQQFETATSLAHVVDQAVIETVQDTAYDTAYPAFFYVVEAMIHGFE
EKFVMNVPLVSLCINTYWERAGRLAFVNSFSMIKFICRHLGNNAISKEAYSMYRKIYGELIALEQALMRL
AGSDVVGDESVGQYVCALLDPNLLPPVAYTDIFTHLLTVSDRAPQIIIGNEVYADTLAAPQFIERVGNMD
EMAAQFVALYGYRVNGDHDHDFRLHLGPYVDEGHADVLEKIFYYVFLPTCTNAHMCGLGVDFQHVAQTLA
YNGPAFSHHFTRDEDILDNLENGTLRDLLEISDLRPTVGMIRDLSASFMTCPTFTRTVRVSVDNDVTQQL
APNPADKRTEQTVLVNGLVAFAFSERTRAVTQCLFHAIPFHMFYGDPRVAATMHQDVATFVMRNPQQRAV
EAFNRPEQLFAEYREWHRSPMGKYAAECLPSLVSISGMTAMHIKMSPMAYIAQAKLKIHPGVAMTVVRTD
EILSENILFSSRASTSMFIGTPNVSRREARVDAVTFEVHHEMASIDTGLSYSSTMTPARVAAITTDMGIH
TQDFFSVFPAEAFGNQQVNDYIKAKVGAQRNGTLLRDPRTYLAGMTNVNGAPGLCHGQQATCEIIVTPVT
ADVAYFQKSNSPRGRAACVVSCENYNQEVAEGLIYDHSRPDAAYEYRSTVNPWASQLGSLGDIMYNSSYR
QTAVPGLYSPCRAFFNKEELLRNNRGLYNMVNEYSQRLGGHPATSNTEVQFVVIAGTDVFLEQPCSFLQE
AFPALSASSRALIDEFMSVKQTHAPIHYGHYIIEEVAPVRRILKFGNKVVF

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_009334
ORF Size 4143 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Reference Data
RefSeq NC_009334.1, YP_001129493
RefSeq ORF 4143
Locus ID 5176226
MW 153.9 kDa

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