BVRF1 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101076

Myc-DDK-tagged ORF clone of viral ORF for BVRF1 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129499

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BVRF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101076 represents NCBI reference of YP_001129499 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACTGAGCGGACACGTGCTGATTGATCCAGCCCGGCTTCCACGCGACACAGGCCCCGAACTTATGT
  GGGCTCCTTCTCTCAGAAATTCCCTCCGGGTAAGTCCGGAGGCGCTCGAGCTGGCAGAGCGGGAGGCAGA
  GAGAGCACGCTCAGAACGCTGGGATCGGTGCGCCCAGGTTCTTAAGAATCGGCTTTTGCGGGTAGAGCTC
  GATGGAATCATGCGCGACCATCTGGCACGGGCAGAAGAAATCCGACAGGACCTGGATGCAGTTGTGGCCT
  TCAGCGACGGACTCGAATCAATGCAGGTAAGAAGCCCTTCCACCGGCGGCCGATCTGCACCAGCCCCCCC
  CAGTCCCTCACCAGCTCAGCCCTTCACAAGGCTGACAGGAAACGCGCAGTATGCTGTAAGTATTAGCCCT
  ACCGATCCTCCGCTGATGGTCGCTGGGAGCCTTGCGCAGACTCTCCTCGGAAATCTCTATGGAAACATTA
  ATCAGTGGGTCCCAAGCTTCGGTCCCTGGTACCGGACCATGAGCGCCAATGCAATGCAGAGGAGAGTTTT
  CCCTAAGCAGTTGCGAGGTAACCTGAACTTCACGAATTCAGTCTCCCTGAAGCTGATGACGGAGGTCGTG
  GCAGTTCTCGAAGGTACGACCCAGGACTTTTTCAGCGACGTTCGCCATCTCCCCGATCTTCAGGCCGCCT
  TGATACTTAGCGTCGCCTACCTGCTTCTGCAGGGAGGATCTTCACACCAACAGCGGCCTCTGCCGGCCAG
  CCGCGAGGAACTCCTGGAGCTCGGGCCAGAGTCATTGGAGAAGATCATCGCAGACCTCAAAGCCAAGAGC
  CCCGGCGGAAACTTCATGATCCTCACTTCTGGTAATAAAGAAGCCCGGCAGTCAATTGCGCCTCTGAATA
  GGCAGGCTGCCTACCCTCCCGGGACATTCGCCGATAATAAGATTTACAACCTGTTCGTTGGTGCGGGCCT
  CCTGCCCACCACTGCCGCCCTGAACGTGCCTGGTGCCGCAGGTCGGGACCGGGATCTCGTTTATAGAATT
  GCTAACCAGATATTTGGCGAGGATGTTCCACCCTTTTCTTCACACCAGTGGAACCTGAGGGTTGGCTTGG
  CAGCATTGGAAGCTCTGATGCTGGTCTACACACTGTGCGAAACAGCAAACTTGGCCGAGGCGGCCACGAG
  ACGACTTCATCTGAGTTCTCTCCTCCCACAAGCCATGCAGCGACGCAAGCCGGCTATGGCAAGCGCCGGA
  ATGCCAGGGGCTTACCCTGTCCAGACTCTGTTCCGGCATGGAGAACTTTTTCGCTTTATTTGGGCGCACT
  ACGTGAGGCCCACCGTGGCTGCAGACCCACAGGCCTCTATCTCTTCCTTGTTCCCAGGCCTGGTGCTGTT
  GGCACTGGAGCTTAAGCTTATGGATGGCCAGGCTCCCAGTCACTATGCAATTAATCTGACTGGACAGAAG
  TTCGACACTTTGTTTGAGATCATCAACCAGAAACTGCTCTTCCACGACCCTGCCGCTATGCTTGCTGCGA
  GGACACAGTTGCGGCTGGCCTTTGAGGACGGAGTGGGAGTGGCTCTTGGCCGACCTTCTCCCATGCTCGC
  GGCCCGGGAAATCCTTGAGAGGCAGTTTTCTGCAAGCGACGACTACGACAGGCTGTACTTCCTGACCCTG
  GGTTACCTGGCCAGCCCGGTGGCACCCTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101076 representing YP_001129499
  Red=Cloning sites Green=Tags
  
  MALSGHVLIDPARLPRDTGPELMWAPSLRNSLRVSPEALELAEREAERARSERWDRCAQVLKNRLLRVEL
  DGIMRDHLARAEEIRQDLDAVVAFSDGLESMQVRSPSTGGRSAPAPPSPSPAQPFTRLTGNAQYAVSISP
  TDPPLMVAGSLAQTLLGNLYGNINQWVPSFGPWYRTMSANAMQRRVFPKQLRGNLNFTNSVSLKLMTEVV
  AVLEGTTQDFFSDVRHLPDLQAALILSVAYLLLQGGSSHQQRPLPASREELLELGPESLEKIIADLKAKS
  PGGNFMILTSGNKEARQSIAPLNRQAAYPPGTFADNKIYNLFVGAGLLPTTAALNVPGAAGRDRDLVYRI
  ANQIFGEDVPPFSSHQWNLRVGLAALEALMLVYTLCETANLAEAATRRLHLSSLLPQAMQRRKPAMASAG
  MPGAYPVQTLFRHGELFRFIWAHYVRPTVAADPQASISSLFPGLVLLALELKLMDGQAPSHYAINLTGQK
  FDTLFEIINQKLLFHDPAAMLAARTQLRLAFEDGVGVALGRPSPMLAAREILERQFSASDDYDRLYFLTL
  GYLASPVAPS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 1710 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009334.1, YP_001129499
• RefSeq ORF: 1710
• Locus ID: 5176157
• MW: 62.5 kDa