single-stranded DNA binding protein (NC_009334) Virus Tagged ORF Clone

CAT#: VC101086

Myc-DDK-tagged ORF clone of viral ORF for BALF2 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129510

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: single-stranded DNA binding protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101086 represents NCBI reference of YP_001129510 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGGGGCGCAAACTTCAGAGGACAATCTGGGCTCTCAGTCACAACCTGGACCCTGTGGATACATTT
  ATTTCTATCCTCTGGCTACTTATCCACTCCGGGAAGTGGCTACCCTCGGGACCGGCTATGCAGGACATCG
  ATGTCTGACTGTCCCACTGCTTTGCGGGATTACCGTCGAGCCCGGCTTTAGCATAAACGTGAAAGCCTTG
  CATCGGCGACCTGATCCAAACTGCGGGCTCCTCCGAGCGACCTCTTACCACAGAGATATCTATGTGTTTC
  ACAATGCCCACATGGTTCCTCCCATCTTTGAAGGGCCTGGTCTGGAGGCCCTGTGCGGAGAGACTCGCGA
  GGTATTTGGTTACGATGCTTACTCCGCCCTCCCGCGAGAGAGCTCAGAACCTGGCGATTTTTTCCCTGAG
  GGATTGGACCCATCCGCGTACTTGGGAGCCGTTGCTATCACGGAGGCCTTTAAGGAGAGACTGTATTCCG
  GGAATCTTGTCGCCATCCCATCTCTGAAGCAAGAGGTGGCCGTGGGTCAGAGCGCATCCGTGCGGGTGCC
  TCTTTACGATAAAGAAGTGTTCCCTGAAGGGGTGCCCCAGCTGAGACAGTTCTATAATTCCGATCTGTCT
  CGCTGCATGCACGAAGCTTTGTACACTGGCCTCGCACAGGCACTCCGCGTACGGCGCGTGGGTAAACTGG
  TCGAGCTGCTGGAAAAGCAAAGTCTCCAGGATCAGGCCAAAGTTGCCAAGGTGGCTCCACTGAAAGAGTT
  CCCCGCAAGTACAATATCTCATCCGGATAGTGGCGCACTCATGATTGTCGATTCTGCCGCTTGCGAACTT
  GCAGTCTCTTACGCCCCTGCCATGCTCGAAGCATCCCATGAGACTCCCGCCTCCCTTAATTACGACTCCT
  GGCCATTGTTCGCTGATTGTGAGGGACCAGAAGCCCGCGTCGCAGCTCTCCACCGGTACAACGCTAGCCT
  GGCCCCGCATGTGAGCACCCAGATTTTTGCTACCAACTCCGTGCTGTACGTCAGTGGGGTATCCAAGAGC
  ACAGGGCAGGGAAAGGAGAGTCTGTTCAACAGCTTCTACATGACACACGGATTGGGCACTCTTCAAGAAG
  GCACATGGGACCCCTGTAGAAGGCCCTGCTTTAGTGGATGGGGCGGACCTGATGTGACTGGGACTAATGG
  CCCAGGGAACTACGCCGTGGAGCATCTGGTATATGCCGCTAGTTTCTCCCCCAACTTGCTGGCCCGGTAT
  GCATACTACCTGCAGTTCTGTCAAGGACAAAAGAGTTCTTTGACCCCTGTACCCGAGACAGGCTCCTACG
  TGGCGGGGGCTGCTGCTTCACCCATGTGTTCCTTGTGTGAGGGTCGAGCGCCTGCAGTTTGTCTGAATAC
  CCTGTTTTTCCGCTTGCGCGATAGATTCCCGCCAGTCATGTCAACTCAACGGCGCGACCCCTATGTTATA
  AGCGGGGCATCTGGGTCTTACAATGAAACCGACTTTCTGGGTAATTTTCTGAATTTTATCGATAAGGAAG
  ATGATGGCCAGAGACCAGATGATGAACCACGGTACACCTACTGGCAACTGAACCAGAATCTGCTGGAAAG
  GCTGAGTCGCCTTGGTATTGACGCTGAAGGGAAGCTCGAGAAAGAGCCACATGGACCTCGAGACTTCGTC
  AAGATGTTCAAGGATGTAGACGCTGCAGTAGATGCCGAAGTGGTGCAATTTATGAACAGCATGGCAAAGA
  ATAACATTACCTACAAAGATCTGGTGAAGAGCTGCTACCATGTTATGCAGTACTCATGCAACCCATTCGC
  ACAACCTGCTTGTCCTATTTTTACTCAGCTCTTCTACCGCAGCCTTCTCACCATCCTGCAGGACATATCC
  CTGCCTATCTGTATGTGCTACGAAAACGATAATCCAGGGCTGGGCCAGTCTCCGCCAGAGTGGTTGAAGG
  GCCACTATCAGACCTTGTGTACTAATTTTAGAAGTCTCGCAATTGACAAGGGCGTTCTCACAGCAAAGGA
  AGCAAAGGTGGTCCACGGCGAGCCAACATGTGACTTGCCTGACCTTGACGCCGCGCTGCAGGGACGAGTC
  TATGGACGCAGACTGCCTGTTCGCATGAGTAAAGTTCTCATGCTGTGCCCACGGAATATTAAAATCAAAA
  ACCGCGTTGTGTTTACCGGAGAAAACGCCGCTCTTCAGAATTCCTTCATCAAGTCCACAACCCGGAGAGA
  GAATTATATCATCAACGGCCCATATATGAAGTTTCTGAACACTTACCACAAAACGCTCTTCCCTGACACT
  AAGCTCTCAAGCCTCTACCTTTGGCACAATTTTTCACGGAGAAGAAGCGTCCCCGTGCCATCAGGAGCAA
  GTGCCGAAGAGTATAGCGACTTGGCTCTCTTTGTAGATGGCGGGTCAAGGGCTCATGAGGAGAGCAATGT
  TATCGATGTCGTCCCTGGGAACTTGGTAACATACGCTAAGCAGCGCTTGAACAACGCAATTCTTAAAGCA
  TGCGGGCAGACCCAATTCTACATCTCCCTCATCCAGGGTTTGGTTCCCAGAACACAATCCGTGCCCGCCC
  GCGACTACCCCCACGTTCTCGGAACCCGGGCTGTCGAGTCCGCTGCTGCCTACGCTGAAGCCACCTCAAG
  CTTGACAGCCACCACGGTAGTGTGTGCAGCTACGGACTGTCTTTCACAGGTCTGTAAAGCGCGGCCTGTG
  GTCACCCTGCCGGTGACGATAAATAAGTACACCGGCGTGAACGGCAATAACCAAATTTTCCAGGCCGGTA
  ATCTCGGATATTTCATGGGAAGAGGAGTTGACAGGAATCTGCTGCAGGCCCCCGGCGCAGGGCTGCGCAA
  ACAGGCAGGGGGTTCCTCAATGAGGAAAAAGTTCGTATTCGCTACCCCTACATTGGGTTTGACTGTGAAG
  CGACGGACTCAAGCCGCCACCACCTACGAAATTGAAAATATCCGGGCTGGACTTGAGGCTATCATCAGTC
  AAAAACAGGAGGAGGACTGTGTTTTCGACGTAGTTTGTAATCTCGTTGACGCGATGGGGGAAGCCTGCGC
  CTCCCTTACGCGGGACGATGCCGAATATCTGCTGGGTCGCTTTTCTGTTCTCGCAGATAGTGTGCTTGAA
  ACACTCGCTACGATCGCATCAAGCGGGATCGAATGGACCGCCGGCGCTGCCCGAGACTTTCTCGAGGGGG
  TGTGGGGGGGTCCAGGCGCAGCGCAAGATAATTTCATTTCTGTGGCTGAACCTGTTTCAACCGCAAGCCA
  GGCTAGTGCAGGCCTCCTGCTGGGAGGAGGCGGGCAGGGGAGCGGAGGCAGGAGAAAACGACGGCTGGCC
  ACAGTCCTGCCTGGCTTGGAAGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101086 representing YP_001129510
  Red=Cloning sites Green=Tags
  
  MQGAQTSEDNLGSQSQPGPCGYIYFYPLATYPLREVATLGTGYAGHRCLTVPLLCGITVEPGFSINVKAL
  HRRPDPNCGLLRATSYHRDIYVFHNAHMVPPIFEGPGLEALCGETREVFGYDAYSALPRESSEPGDFFPE
  GLDPSAYLGAVAITEAFKERLYSGNLVAIPSLKQEVAVGQSASVRVPLYDKEVFPEGVPQLRQFYNSDLS
  RCMHEALYTGLAQALRVRRVGKLVELLEKQSLQDQAKVAKVAPLKEFPASTISHPDSGALMIVDSAACEL
  AVSYAPAMLEASHETPASLNYDSWPLFADCEGPEARVAALHRYNASLAPHVSTQIFATNSVLYVSGVSKS
  TGQGKESLFNSFYMTHGLGTLQEGTWDPCRRPCFSGWGGPDVTGTNGPGNYAVEHLVYAASFSPNLLARY
  AYYLQFCQGQKSSLTPVPETGSYVAGAAASPMCSLCEGRAPAVCLNTLFFRLRDRFPPVMSTQRRDPYVI
  SGASGSYNETDFLGNFLNFIDKEDDGQRPDDEPRYTYWQLNQNLLERLSRLGIDAEGKLEKEPHGPRDFV
  KMFKDVDAAVDAEVVQFMNSMAKNNITYKDLVKSCYHVMQYSCNPFAQPACPIFTQLFYRSLLTILQDIS
  LPICMCYENDNPGLGQSPPEWLKGHYQTLCTNFRSLAIDKGVLTAKEAKVVHGEPTCDLPDLDAALQGRV
  YGRRLPVRMSKVLMLCPRNIKIKNRVVFTGENAALQNSFIKSTTRRENYIINGPYMKFLNTYHKTLFPDT
  KLSSLYLWHNFSRRRSVPVPSGASAEEYSDLALFVDGGSRAHEESNVIDVVPGNLVTYAKQRLNNAILKA
  CGQTQFYISLIQGLVPRTQSVPARDYPHVLGTRAVESAAAYAEATSSLTATTVVCAATDCLSQVCKARPV
  VTLPVTINKYTGVNGNNQIFQAGNLGYFMGRGVDRNLLQAPGAGLRKQAGGSSMRKKFVFATPTLGLTVK
  RRTQAATTYEIENIRAGLEAIISQKQEEDCVFDVVCNLVDAMGEACASLTRDDAEYLLGRFSVLADSVLE
  TLATIASSGIEWTAGAARDFLEGVWGGPGAAQDNFISVAEPVSTASQASAGLLLGGGGQGSGGRRKRRLA
  TVLPGLEV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 3384 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009334.1, YP_001129510
• RefSeq ORF: 3384
• Locus ID: 5176206
• MW: 123.1 kDa