BGLF4 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101059

Myc-DDK-tagged ORF clone of viral ORF for BGLF4 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129482

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BGLF4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101059 represents NCBI reference of YP_001129482 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGTGAACATGGCGGCTGAGCTGTCACCAACTAATAGTAGCAGTAGCGGGGAACTGTCAGTGTCAC
  CAGAACCACCACGCGAGACACAGGCATTCCTGGGCAAGGTGACCGTCATCGATTACTTCACTTTCCAGCA
  CAAGCATTTGAAAGTCACAAACATTGACGATATGACCGAGACTTTGTATGTAAAGCTGCCAGAGAATATG
  ACGAGGTGTGACCACCTGCCGATAACCTGCGAGTATCTTCTGGGACGCGGGTCCTACGGCGCCGTGTACG
  CACATGCTGACAACGCAACCGTTAAACTTTATGACAGCGTTACCGAGCTGTACCATGAGCTGATGGTGTG
  CGACATGATACAAATCGGCAAAGCTACGGCCGAAGATGGACAAGATAAGGCCCTGGTTGATTACCTGTCA
  GCCTGCACCTCATGTCACGCACTGTTCATGCCACAATTCAGGTGCAGCCTCCAAGATTACGGTCACTGGC
  ACGACGGTTCAATCGAGCCACTTGTCAGGGGTTTTCAAGGGCTCAAAGACGCCGTGTATTTCCTCAACCG
  CCACTGTGGCCTTTTCCACTCTGATATTAGCCCTAGCAACATTTTGGTGGATTTTACGGACACAATGTGG
  GGCATGGGACGGCTTGTGTTGACCGATTACGGGACCGCAAGTCTGCACGATCGAAATAAAATGCTGGACG
  TGCGGCTCAAGTCAAGCAAAGGCAGACAACTGTATAGGCTGTATTGTCAGCGGGAGCCTTTCAGTATTGC
  CAAAGACACCTACAAACCCCTGTGCCTCCTTTCAAAATGCTACATACTGCGGGGCGCCGGACACATCCCT
  GATCCCTCCGCCTGCGGTCCCGTCGGGGCACAGACAGCTTTGCGGCTTGATTTGCAAAGTCTGGGATATT
  CTCTGCTCTACGGTATTATGCATCTGGCTGACTCAACTCACAAGATCCCCTATCCCAATCCCGATATGGG
  CTTCGACAGATCCGACCCCCTTTACTTCCTTCAATTTGCTGCTCCGAAAGTGGTGCTGTTGGAGGTATTG
  TCTCAGATGTGGAATTTGAACTTGGATATGGGCCTGACGAGTTGCGGCGAATCCCCCTGCGTGGACGTCA
  CCGCTGAGCACATGTCCCAGTTCCTGCAGTGGTGCCGGAGCCTCAAGAAGAGATTTAAAGAAAGTTACTT
  CTTTAACTGCAGGCCCAGATTCGAGCACCCTCACCTGCCCGGCTTGGTGGCGGAACTGTTGGCTGATGAC
  TTCTTCGGTCCCGACGGTAGACGAGGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101059 representing YP_001129482
  Red=Cloning sites Green=Tags
  
  MDVNMAAELSPTNSSSSGELSVSPEPPRETQAFLGKVTVIDYFTFQHKHLKVTNIDDMTETLYVKLPENM
  TRCDHLPITCEYLLGRGSYGAVYAHADNATVKLYDSVTELYHELMVCDMIQIGKATAEDGQDKALVDYLS
  ACTSCHALFMPQFRCSLQDYGHWHDGSIEPLVRGFQGLKDAVYFLNRHCGLFHSDISPSNILVDFTDTMW
  GMGRLVLTDYGTASLHDRNKMLDVRLKSSKGRQLYRLYCQREPFSIAKDTYKPLCLLSKCYILRGAGHIP
  DPSACGPVGAQTALRLDLQSLGYSLLYGIMHLADSTHKIPYPNPDMGFDRSDPLYFLQFAAPKVVLLEVL
  SQMWNLNLDMGLTSCGESPCVDVTAEHMSQFLQWCRSLKKRFKESYFFNCRPRFEHPHLPGLVAELLADD
  FFGPDGRRG
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 1287 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009334.1, YP_001129482
• RefSeq ORF: 1287
• Locus ID: 5176213
• MW: 48.4 kDa