BDLF2 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101068

Myc-DDK-tagged ORF clone of viral ORF for BDLF2 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129491

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BDLF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101068 represents NCBI reference of YP_001129491 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGACGAACAGGTAGCCGTGGAGCACGGAACAGTCTCCCACACAATTTCTCGAGAGGAGGATGGCG
  TTGTGCACGAAAGGCGCGTGCTGGCTAGTGGGGAAAGGGTCGAGGTGTTTTACAAAGCGCCAGCGCCGAG
  GCCTCGAGAAGGCCGGGCTTCAACATTCCATGATTTTACAGTGCCTGCAGCCGCCGCTGTACCGGGTCCC
  GAGCCGGAACCGGAGCCCCACCCTCCGATGCCTATCCACGCTAATGGCGGGGGCGAGACCAAGACAAATA
  CCCAGGATCAAAATCAAAATCAGACCACGAGAACTAGAACCAATGCAAAGGCTGAGGAGCGGACCGCCGA
  GATGGATGACACAATGGCTTCTTCCGGAGGACAGCGAGGCGCTCCTATTTCTGCCGACTTGCTGTCTCTG
  AGCTCTTTGACAGGCAGGATGGCAGCGATGGCTCCCTCCTGGATGAAATCTGAGGTCTGCGGCGAACGGA
  TGCGCTTCAAAGAGGACGTGTACGATGGAGAAGCCGAGACACTGGCGGAACCTCCCAGGTGTTTTATGCT
  GTCTTTCGTTTTCATCTACTACTGTTGCTACCTTGCATTTCTGGCCCTGCTCGCCTTCGGATTTAACCCT
  CTGTTCCTCCCTTCTTTTATGCCGGTGGGAGCAAAAGTTCTCCGAGGAAAAGGCAGAGACTTCGGAGTTC
  CACTGAGTTACGGGTGCCCAACCAATCCATTCTGCAAGGTGTACACCCTGATCCCAGCCGTAGTCATCAA
  CAACGTTACGTATTACCCCAATAATACTGATTCCCACGGAGGGCATGGGGGCTTCGAAGCAGCCGCACTG
  CACGTTGCAGCTTTGTTTGAATCCGGTTGCCCCAACTTGCAAGCCGTTACAAATAGAAATAGAACCTTCA
  ATGTCACCAGAGCCTCTGGGAGAGTCGAACGCAGACTGGTGCAGGATATGCAGCGCGTGTTGGCAAGCGC
  TGTCGTGGTGATGCACCACCACTGCCATTATGAGACCTATTACGTTTTTGATGGGGTTGGGCCAGAATTT
  GGCACCATCCCAACACCATGCTTTAAAGATGTGCTGGCTTTTAGGCCGAGCCTCGTCACAAATTGCACCG
  CTCCCTTGAAAACGTCAGTGAAGGGGCCAAACTGGTCTGGCGCAGCTGGAGGCATGAAGCGGAAGCAGTG
  TCGCGTCGACAGACTCACGGATCGGAGCTTCCCTGCATACCTCGAGGAAGTCATGTACGTTATGGTGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101068 representing YP_001129491
  Red=Cloning sites Green=Tags
  
  MVDEQVAVEHGTVSHTISREEDGVVHERRVLASGERVEVFYKAPAPRPREGRASTFHDFTVPAAAAVPGP
  EPEPEPHPPMPIHANGGGETKTNTQDQNQNQTTRTRTNAKAEERTAEMDDTMASSGGQRGAPISADLLSL
  SSLTGRMAAMAPSWMKSEVCGERMRFKEDVYDGEAETLAEPPRCFMLSFVFIYYCCYLAFLALLAFGFNP
  LFLPSFMPVGAKVLRGKGRDFGVPLSYGCPTNPFCKVYTLIPAVVINNVTYYPNNTDSHGGHGGFEAAAL
  HVAALFESGCPNLQAVTNRNRTFNVTRASGRVERRLVQDMQRVLASAVVVMHHHCHYETYYVFDGVGPEF
  GTIPTPCFKDVLAFRPSLVTNCTAPLKTSVKGPNWSGAAGGMKRKQCRVDRLTDRSFPAYLEEVMYVMVQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 1260 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009334.1, YP_001129491
• RefSeq ORF: 1260
• Locus ID: 5176177
• MW: 46.2 kDa