BFRF1 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101023

Myc-DDK-tagged ORF clone of viral ORF for BFRF1 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129446

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BFRF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101023 represents NCBI reference of YP_001129446 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCACCTGAAGAGCGACTGCTGGATGAATTGAATAACTTGATCGTGTCCTTTCTCTGTGACTCTG
  GAAGCCTGGAGGTTGAGAGGTGCTCTGGAGCACATGTCTTTAGTCGGGGCAGCAGCCAGCCACTGTGCAC
  TGTGAAACTGAGACATGGCCAGATATATCACCTGGAGTTCGTGTATAAATTCCTGGCATTCAAGCTGAAG
  AACTGTAACTACCCATCCTCTCCCGTGTTTGTTATTTCTAATAATGGCCTGGCCACTACCCTCAGATGCT
  TCCTGCACGAACCCTCCGGCCTCAGATCCGGTCAGAGCGGCCCCTGTCTGGGCCTGAGCACCGATGTTGA
  TCTCCCCAAGAATAGCATTATTATGCTCGGCCAGGATGACTTCATTAAGTTCAAATCTCCTCTGGTGTTT
  CCTGCTGAGCTGGACCTCCTGAAGAGTATGGTTGTTTGCAGAGCGTACATCACTGAACATCGGACCACGA
  TGCAGTTCCTGGTGTTTCAGGCGGCGAACGCACAGAAAGCAAGTCGGGTGATGGATATGATTTCTGACAT
  GAGCCAACAGTTGAGTAGATCTGGGCAGGTGGAGGATACAGGCGCAAGAGTTACTGGCGGCGGGGGACCG
  CGCCCCGGAGTCACACACTCTGGGTGTCTGGGTGATAGTCATGTACGGGGCAGGGGGGGCTGGGACTTGG
  ACAACTTCAGCGAGGCGGAAACAGAAGATGAGGCCAGCTACGCCCCCTGGCGGGATAAAGACAGTTGGTC
  CGAGAGTGAGGCTGCCCCCTGGAAGAAGGAGCTGGTGCGGCATCCTATTCGGAGACACCGCACAAGAGAA
  ACCCGCCGGATGCGCGGCTCTCATTCCAGGGTAGAGCACGTTCCTCCAGAGACTCGAGAGACAGTAGTCG
  GGGGAGCGTGGAGGTACTCCTGGAGGGCTACTCCTTATCTTGCTCGAGTGCTCGCCGTGACAGCCGTTGC
  CTTGCTGCTTATGTTTTTGCGCTGGACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101023 representing YP_001129446
  Red=Cloning sites Green=Tags
  
  MASPEERLLDELNNLIVSFLCDSGSLEVERCSGAHVFSRGSSQPLCTVKLRHGQIYHLEFVYKFLAFKLK
  NCNYPSSPVFVISNNGLATTLRCFLHEPSGLRSGQSGPCLGLSTDVDLPKNSIIMLGQDDFIKFKSPLVF
  PAELDLLKSMVVCRAYITEHRTTMQFLVFQAANAQKASRVMDMISDMSQQLSRSGQVEDTGARVTGGGGP
  RPGVTHSGCLGDSHVRGRGGWDLDNFSEAETEDEASYAPWRDKDSWSESEAAPWKKELVRHPIRRHRTRE
  TRRMRGSHSRVEHVPPETRETVVGGAWRYSWRATPYLARVLAVTAVALLLMFLRWT
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 1008 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009334.1, YP_001129446
• RefSeq ORF: 1008
• Locus ID: 5176178
• MW: 37.6 kDa