helicase-primase subunit BBLF4 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101052

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for BBLF4 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129475


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Reconstitution Protocol

USD 1,290.00

5 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Symbol helicase-primase subunit BBLF4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101052 represents NCBI reference of YP_001129475 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGAGGAGCCAAGGGCTCCCGAGGCCCTGTCAAGCACTTTCATGCTGAACATGACATCAGACGCCT
CTGTTCGCCGAATCGTTCGGCGAATCGGCACACTGGCCAAGCGGAGAGTGCAGCAGCTGCCAGACATGGA
AACCTTCTCACCCGAGTTTGATCCCGAAATTTCCGAGCCTCCCTTCCTGCCATTTTCCGCCTACGTGATT
ACTGGTACAGCCGGCGCTGGGAAGTCCACTTCTGTGTCATGCCTCCATCATACAATGGATTGTTTGGTGA
CAGGAGCCACAACCGTCGCGGCGCAGAACCTCAGTCAAACCCTCAGAGCGTACTGCCCGACAGTGTACAG
CGCGTTTGGCTTTAAAAGCCGCCACATCAACATGACACAGAGAGTTTCCTCACATGGTAGGAGCACCGAC
GCCGCTCTTGAAGAACTTCAGCGCCGCGACATGGCTAAGTATTGGCCAGTGTTGTCAGATATCGCAGCCG
AGTTTCGCCGGACAAAGCCAAGGGGACTTTATTCTGGTGTGTCCGGGCCTGCATTCGAAGTGTTGAGGGA
TATGCACCAAGGCCAATTGTGGACAACGAATGTGATCGTGGTGGACGAGGCAGGAACTCTGTCCGTACAT
ATCCTGACCGCTGTTGTTTTTTGCTATTGGTTTTTCAATGCATGGCTCAGAACCCCTCTCTACAGGAGAG
GACGGATCCCTTGCATCGTGTGCGTTGGTTCACCCACCCAAACAGATGCCTTCCAATCTAGCTTTAGCCA
TGAAACACAAGTGAATAAGATAAGAGAATGTGACAACATTCTTACTTTCCTTGTGGGCAACCCTAGAGCC
GCCACTTACGTCGACGTAGCACGGAACTGGGCATTGTTTATAAATAACAAACGGTGTACCGACGTGCAAT
TCGGACACCTGATGAAAACCCTGGAATACGGACTCGAGCTGTCTCCAGATATCCTGGCGTACGTGGATCG
CTTCGTAGTTCCACGCGCGGCTATCATGGATCCAACACAGTACGTGGGATGGACACGGCTTTTCCTTTCC
CATGCCGAAGTGAAGACTTTCCTCACCACTCTCCACGCTACACTCAAGACCGCCGGTCAGGGCAGAGCCG
CTAGGGGAACCGGGGGAGACGGAGGAGGGGTGACAATGTTTACTTGTCCCGTAGAGTGCGAGGTTTTTCT
TGAGCCCTTGGCTCAATATAAGACCCTGGTAGGACTGCCTGGTCTTACAGCTCACACATGGCTGCAGAAA
AATTATGCACGCCTGGGCAACTATAGTCAATTTGCCGACCAGGATATGGTCCCTGTGGGGACAGAACAGG
ATGAAGAGAGGGTTAAGGTGACCTATAATGTGACTTACGTTAAGCACTCAAGCGTGAGTGTAAACTGCAA
AACCAAGAAGAGCATTTGCGGATACACGGGCACCTTCGGGGACTTCATGGACACACTTGAGGCTGATTCC
TTCGTGGAGGCACACGGGCATGAGCAACCTGAGTATGTGTATTCATTTCTCGCCAGGCTTATCTATGGTG
GAATCTACGCATTTAGCCACGGAGGACATTCCCTCTGTGAAAACGGGGAATACGTGGCTGAGCTTGGTGC
CGTGCCGCTGCCAGGTAGGACCTGGGACCCCGAAGTTACGGCTGGCATGGAGCTCGGAGAGTTGCCTCTG
GAGGTCGCCTGGGATGGAGAGCGCAGTCCTGCTGCCGTGTTCTACGCGAGGGTGCTGGCACCCCCAGCCG
CCAACAGCGCTCCCCTGTGCTCACTGCTGAATATATACAACGACCTGAGGGCTTATTTTCGCCAGTGCCT
TGATGTCGCCGTTAGGTATGGGCGCCGCGAGTTCCGCGACCTTCCGTTTTGCACCTTCACCAATAATATG
CTCATTAGAGATAATATCGAGTTCACCAGCGACGAGCCGCTGTTGCATGGGCTCTTGGACTATGCCTCCA
CTACCGAGAACTATACACTGCTCGGCTATACCCACCTGAACGTTTTTTTTGGAATTAGAGGCAAACAACA
GCCTCAAGACGCAGGCTCATCAAGAATGCCGAGACTGATGGTCAAGGATGAAGCAGGGTTTGTGTGCTGC
CTGGAACACAATACTAATAAACTGTACGAGACAATCGAGGACAAAAGTCTGAACTTGTGCTCAATACGAG
ACTACGGGATAAGCAGTAAGCTTGCTATGACGATCGCTAAGGCTCAAGGGCTGTCCCTTAACAAGGTTGC
CATATGTTTTGGCTCCCATAGAAACATTAAGCCTGGGCATGTTTACGTTGCACTTAGCCGGGCGCGCCAT
TCCAACTGCGTTGTAATGGATAGAAACCCCCTGTCTGAAATGATAACCGGAGAGGGAAACCCAGCAAGCG
GATATATTGTTGATGCCCTGAAAAATTCCCGGGCTTTGCTGGTGTAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101052 representing YP_001129475
Red=Cloning sites Green=Tags

MAEEPRAPEALSSTFMLNMTSDASVRRIVRRIGTLAKRRVQQLPDMETFSPEFDPEISEPPFLPFSAYVI
TGTAGAGKSTSVSCLHHTMDCLVTGATTVAAQNLSQTLRAYCPTVYSAFGFKSRHINMTQRVSSHGRSTD
AALEELQRRDMAKYWPVLSDIAAEFRRTKPRGLYSGVSGPAFEVLRDMHQGQLWTTNVIVVDEAGTLSVH
ILTAVVFCYWFFNAWLRTPLYRRGRIPCIVCVGSPTQTDAFQSSFSHETQVNKIRECDNILTFLVGNPRA
ATYVDVARNWALFINNKRCTDVQFGHLMKTLEYGLELSPDILAYVDRFVVPRAAIMDPTQYVGWTRLFLS
HAEVKTFLTTLHATLKTAGQGRAARGTGGDGGGVTMFTCPVECEVFLEPLAQYKTLVGLPGLTAHTWLQK
NYARLGNYSQFADQDMVPVGTEQDEERVKVTYNVTYVKHSSVSVNCKTKKSICGYTGTFGDFMDTLEADS
FVEAHGHEQPEYVYSFLARLIYGGIYAFSHGGHSLCENGEYVAELGAVPLPGRTWDPEVTAGMELGELPL
EVAWDGERSPAAVFYARVLAPPAANSAPLCSLLNIYNDLRAYFRQCLDVAVRYGRREFRDLPFCTFTNNM
LIRDNIEFTSDEPLLHGLLDYASTTENYTLLGYTHLNVFFGIRGKQQPQDAGSSRMPRLMVKDEAGFVCC
LEHNTNKLYETIEDKSLNLCSIRDYGISSKLAMTIAKAQGLSLNKVAICFGSHRNIKPGHVYVALSRARH
SNCVVMDRNPLSEMITGEGNPASGYIVDALKNSRALLVY

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_009334
ORF Size 2427 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Reference Data
RefSeq NC_009334.1, YP_001129475
RefSeq ORF 2427
Locus ID 5176217
MW 90.0 kDa

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