BFRF3 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101025

Myc-DDK-tagged ORF clone of viral ORF for BFRF3 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129448

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BFRF3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101025 represents NCBI reference of YP_001129448 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACGCCGACTGCCGAAGCCTACCTTGCAGGGGAGGCTGGAGGCAGACTTTCCCGACAGTCCCCTTC
  TCCCAAAATTTCAGGAACTGAACCAGAACAATCTTCCCAATGACGTGTTCAGGGAGGCCCAGCGGTCCTA
  CCTGGTATTTCTTACTTCTCAATTCTGTTACGAGGAGTACGTGCAGCGGACATTTGGTGTGCCCCGCAGA
  CAGAGAGCTATAGATAAGAGGCAGAGAGCTTCTGTAGCAGGCGCAGGCGCTCACGCTCATCTGGGAGGGT
  CTTCTGCCACACCGGTCCAACAGGCACAGGCTGCCGCTTCCGCAGGCACGGGTGCCCTCGCATCTTCCGC
  TCCTAGCACCGCAGTGGCCCAGTCTGCAACCCCCTCTGTCTCCTCTTCTATTAGCTCACTTCGGGCAGCG
  ACATCTGGGGCAACCGCTGCCGCCTCTGCCGCGGCAGCAGTGGATACTGGCTCTGGTGGAGGAGGTCAGC
  CACAAGACACTGCGCCAAGGGGCGCAAGAAAGAAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101025 representing YP_001129448
  Red=Cloning sites Green=Tags
  
  MARRLPKPTLQGRLEADFPDSPLLPKFQELNQNNLPNDVFREAQRSYLVFLTSQFCYEEYVQRTFGVPRR
  QRAIDKRQRASVAGAGAHAHLGGSSATPVQQAQAAASAGTGALASSAPSTAVAQSATPSVSSSISSLRAA
  TSGATAAASAAAAVDTGSGGGGQPQDTAPRGARKKQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 528 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009334.1, YP_001129448
• RefSeq ORF: 528
• Locus ID: 5176190
• MW: 18.1 kDa