BSRF1 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101035

Myc-DDK-tagged ORF clone of viral ORF for BSRF1 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129458

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BSRF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101035 represents NCBI reference of YP_001129458 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTTCTATCTCCCCGACTGGAGCTGTTGTGGCCTCTGGCTGTTCGGTCGGCCTAGAAACAGGTACT
  CACAGCTGCCCGAAGAACCTGAAACCTTCGAATGTCCCGACAGATGGCGAGCTGAAATTGATCTTGGGCT
  CCCCCCAGGAGTGCAGGTGGGGGACCTCCTGCGGAATGAGCAGACCATGGGATCACTGAGGCAGGTCTAT
  CTGCTGGCCGTGCAGGCCAATAGTATAACCGACCACCTGAAGAGGTTCGACGCTGTGCGCGTTCCAGAAT
  CCTGTAGAGGCGTGGTGGAAGCTCAGGTTGCGAAATTGGAAGCAGTGCGCAGTGTTATTTGGAACACCAT
  GATCAGCCTCGCCGTGTCCGGCATTGAGATGGACGAGAACGGGCTTAAAGCACTGCTTGACAAACAAGCG
  GGGGATTCCCTGGCCTTGATGGAGATGGAAAAAGTCGCAACTGCACTCAAGATGGACGAAACTGGGGCCT
  GGGCACAGGAGATTAGCGCAGTGGTCAGCAGCGTGACAGCACCTTCAGCTAGCGCTCCTTTTATTAACTC
  CGCCTTCGAGCCTGAAGTGCCGACTCCCGTCTTGGCCCCTCCACCCGTTGTTCGCCAACCTGAGCATAGC
  GGTCCAACTGAGCTCGCTCTGACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101035 representing YP_001129458
  Red=Cloning sites Green=Tags
  
  MAFYLPDWSCCGLWLFGRPRNRYSQLPEEPETFECPDRWRAEIDLGLPPGVQVGDLLRNEQTMGSLRQVY
  LLAVQANSITDHLKRFDAVRVPESCRGVVEAQVAKLEAVRSVIWNTMISLAVSGIEMDENGLKALLDKQA
  GDSLALMEMEKVATALKMDETGAWAQEISAVVSSVTAPSASAPFINSAFEPEVPTPVLAPPPVVRQPEHS
  GPTELALT
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 654 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009334.1, YP_001129458
• RefSeq ORF: 654
• Locus ID: 5176173
• MW: 23.9 kDa