hypothetical protein (NC_009334) Virus Tagged ORF Clone

CAT#: VC101071

Myc-DDK-tagged ORF clone of viral ORF for BcRF1 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129494

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: hypothetical protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101071 represents NCBI reference of YP_001129494 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTCAGGGCAAACGCGAGATGGGTGGCGGTCTGGAAGGCTTTTCTTCTCAGCTGGGGCTGTTTTACG
  CACTGGCTTGTAACAGATCACCTCCTGCACTGCCTGAAGACGCAACATTGCTGATTAAGTGGCTGGATAC
  CGCTCTGGGTCGCGAGGCCACTTTTTACGCATGCCGGGCTATGAGGCGGCTTCTGCTCGGCGTGATTAGG
  ATCAACGACTGCCAGGAGCTCCCTCCTGGCCTGATAATCCTGTCTCCGGGCACAGTCCCCGGTCCTCTCG
  GCGTCCAGTCCCTCGAGCATACCGATTGTGAAATCTGGTCCTCCGCTCATCCAGACCATGCTGCCCACCT
  GCCTGTGCCCAGGGTAATCACTTATACAGACTGTCCCGGCTCTATCAGCACTTCCAGCATGTTCCGACTG
  ATAATTCGCTACCTGTCACACCACCAATTCGAAAGATGCTTCGAGCAGTTTTGCAGAGTCGTTCCCCGCC
  GCTTCCTGGGCACGTGCAAACAAAACTCTGCGAAGATGCTTGCTCACTTGAAACAGGTTACCAGAATCCC
  ACCCTGTCCCCCTTTTAGCGGTAGGGAGGCGAGGTTGAAATTCCACTTCTTCAGTTGGTCTACTTTTATG
  CTCTCTTGGCCAAACAATGCGACGCTCAGGGAAATACGCACAAGGGCTGCCACGAACCTGACACACCACC
  CCCACCTGGTGGATACTCTGTACCACGCATCTCCCCAAACCCCCTTTCTCACTCGCAGCGGAGCTCTGTA
  TAGGTTCGTGACATGTTGCAATTGCACCCTGCCTAATATTAGCATCCAGCAGTGCAAAGCAGGTGATAGA
  CCTGGCGACTTGGAGATAATCCTGCAGAGCAATGGGGGAGGTCGGCCAGCTTCCTTCCAGTTCCCATCCT
  CACCCACAGGAGCCCTTCTGCGGTGCATCGTGGCTGCCGCCCTGCTGCCCGAGGTCTCAGTGGGCCACCA
  AGAGCTTTCACCTCTCATGTCCCGATCCCACGGCGGACAAACCGACGTCCGCAGCGGACCAGACCCTGCC
  CGGCGCCTTGTCGCTTTGCTCCGGAGAGAAGACGGAGCTCCCAAGGATCCTCCTTTGGGACCCTTCGGGC
  ACCCCAGAGGACCAGGCCCCGCAAAGAGTGAAGATGAAGAATCAGAGCGCCGGGACGCGCCACCGCCCCC
  TCTGGACTTCAGCTTTCAGGCTTCACGGTTGGTGCCGGTCGGACCCGGGTTCAGACTTTTGGTTTTCAAT
  ACAAACAGAGTAATCAACACAAAACTTGTCTGTAGTGAGCCACTCGTGAAAATGCGGGTGTGCAACGTCC
  CCCGATTGATCAACAACTTTGTCGCAAGGAAATACGTAGTGAAGGAAACAGCGTTTACAGTTTCTCTCTT
  TTTTACCGACGGAGTGGGCGCCAACCTGGCCATTAATGTGAATATATCCGGCACATACTTGTCCTTTCTC
  CTGGCGATGACTAGCCTCCGGTGCTTTTTGCCAGTCGAGGCCATCTACCCCGCAGCTGTTAGCAATTGGA
  ACTCCACACTCGATCTGCACGGTCTGGAAAACCAGTCTTTGGTTCGCGAAAACAGGAGCGGCGTCTTTTG
  GACGACTAACTTTCCTTCAGTCGTGTCTTGTCAGGACGGGCTGAACGTTTCATGGTTCAAAGCCGCCACT
  GCTACGATTTCAAGGGTCCACGGCCGAACTCTCGAGCAACATCTTATACGCGAAATCACCCCCATCGTCA
  CTCACCGAGAGGCCAAGATATCCCGCATCAAGAACCGGCTCTTCACGTTGCTTGAACTGAGGAACCGATC
  ACAGATCCAGGTTCTCCATAAGCGCTTCCTTGAGGGACTGCTGGACTGCGCCTCTCTTCTGCGGCTGGAC
  CCTTCATGCATCAATAGAATCGCCTCCGAGGGCCTGTTCGACTTCAGCAAGAGGTCAATCGCCCACTCTA
  AGAATCGCCACGAGTGCGCTCTCCTGGGTCATCGGCATTCAGCTAATGTTACCAAGCTCGTTGTGAACGA
  AAGGAAAACCCGGCTTGATATTCTGGGACGAAACGCTAACTTTCTGACACGATGCAAACACCAGGTGAAT
  CTCAGACAGTCACCTATCTTCCTTACCCTTCTCCGCCATATCCGCCGCCGGCTTGGGCTGGGCCGGGCCA
  GCGTGAAGCGGGAAATCACTCTGCTGCTGGCTCACCTCCGGAAGAAAACGGCCCCAATTCACTGTAGAGA
  TGCTCAGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101071 representing YP_001129494
  Red=Cloning sites Green=Tags
  
  MTQGKREMGGGLEGFSSQLGLFYALACNRSPPALPEDATLLIKWLDTALGREATFYACRAMRRLLLGVIR
  INDCQELPPGLIILSPGTVPGPLGVQSLEHTDCEIWSSAHPDHAAHLPVPRVITYTDCPGSISTSSMFRL
  IIRYLSHHQFERCFEQFCRVVPRRFLGTCKQNSAKMLAHLKQVTRIPPCPPFSGREARLKFHFFSWSTFM
  LSWPNNATLREIRTRAATNLTHHPHLVDTLYHASPQTPFLTRSGALYRFVTCCNCTLPNISIQQCKAGDR
  PGDLEIILQSNGGGRPASFQFPSSPTGALLRCIVAAALLPEVSVGHQELSPLMSRSHGGQTDVRSGPDPA
  RRLVALLRREDGAPKDPPLGPFGHPRGPGPAKSEDEESERRDAPPPPLDFSFQASRLVPVGPGFRLLVFN
  TNRVINTKLVCSEPLVKMRVCNVPRLINNFVARKYVVKETAFTVSLFFTDGVGANLAINVNISGTYLSFL
  LAMTSLRCFLPVEAIYPAAVSNWNSTLDLHGLENQSLVRENRSGVFWTTNFPSVVSCQDGLNVSWFKAAT
  ATISRVHGRTLEQHLIREITPIVTHREAKISRIKNRLFTLLELRNRSQIQVLHKRFLEGLLDCASLLRLD
  PSCINRIASEGLFDFSKRSIAHSKNRHECALLGHRHSANVTKLVVNERKTRLDILGRNANFLTRCKHQVN
  LRQSPIFLTLLRHIRRRLGLGRASVKREITLLLAHLRKKTAPIHCRDAQV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 2250 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009334.1, YP_001129494
• RefSeq ORF: 2250
• Locus ID: 5176153
• MW: 83.8 kDa