BGLF5 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101058

Myc-DDK-tagged ORF clone of viral ORF for BGLF5 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129481

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BGLF5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101058 represents NCBI reference of YP_001129481 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACGTTGACGAGTTGGAAGATCCAATGGAAGAGATGACTTCATATACATTCGCAAGATTCCTCA
  GAAGCCCTGAAACAGAAGCCTTTGTTAGAAATTTGGACCGGCCTCCGCAGATGCCCGCCATGAGATACGT
  GTATCTGTACTGCCTCTGTGAGCAAATCCAAGAGTTTAGCGGCGAGACGGGCTTCTGCGACTTTGTGTCT
  AGCCTGGTTCAGGAAAATGACTCACAGGACGGACCAAGCCTGAAGTCCATCTATTGGGGACTGCAAGAGG
  CCACCGATGAACAACGCACAGTCCTGTGCTCCTACGTAGAGTCAATGACTCGCGGTCAGTCCGAGAACCT
  TATGTGGGACATACTTAGAAACGGAATTATAAGCTCCTCAAAACTGCTCAGTACCATTAAGAACGGTCCC
  ACAAAAGTATTCGAGCCCGCTCCCATCAGTACCAATCACTACTTTGGTGGCCCTGTTGCCTTCGGTCTGC
  GGTGCGAGGATACGGTAAAAGATATCGTTTGTAAGCTCATCTGTGGAGATGCTTCTGCAAATCGCCAGTT
  CGGCTTTATGATCTCTCCAACGGACGGCATATTCGGGGTGTCTCTGGATTTGTGTGTCAACGTAGAGTCC
  CAGGGAGATTTCATTCTGTTCACCGACAGATCCTGCATTTATGAGATCAAATGCCGGTTTAAATACCTGT
  TCTCCAAAAGTGAATTCGATCCTATTTACCCAAGTTACACTGCCCTGTATAAACGACCATGCAAGAGAAG
  CTTCATCCGCTTCATAAACTCTATCGCCAGGCCCACTGTGGAGTACGTGCCAGACGGACGATTGCCCTCT
  GAGGGAGACTACCTGCTGACCCAGGACGAAGCCTGGAATCTGAAGGATGTGCGAAAGCGGAAGCTTGGTC
  CTGGACACGATCTCGTGGCAGATTCCCTCGCAGCCAACCGGGGCGTTGAGAGTATGCTGTATGTCATGAC
  AGACCCGAGCGAAAACGCAGGTAGAATCGGCATCAAAGACCGCGTGCCCGTTAATATATTCATTAACCCT
  CGACACAATTACTTCTACCAAGTTCTGCTGCAGTATAAGATAGTTGGCGACTATGTCCGCCATTCAGGGG
  GGGGCAAACCAGGCCGGGATTGCAGTCCCCGAGTGAACATCGTTACTGCCTTCTTTCGAAAAAGAAGCCC
  GCTCGACCCTGCAACCTGTACATTGGGATCTGATCTTCTTCTGGACGCCTCCGTGGAGATACCCGTTGCC
  GTACTGGTGACCCCTGTCGTGCTCCCTGATTCCGTCATTCGGAAGACATTGAGCACCGCTGCTGGTTCTT
  GGAAAGCCTACGCCGACAACACTTTTGACACCGCCCCATGGGTTCCGTCTGGCTTGTTTGCTGACGACGA
  GTCAACCCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101058 representing YP_001129481
  Red=Cloning sites Green=Tags
  
  MADVDELEDPMEEMTSYTFARFLRSPETEAFVRNLDRPPQMPAMRYVYLYCLCEQIQEFSGETGFCDFVS
  SLVQENDSQDGPSLKSIYWGLQEATDEQRTVLCSYVESMTRGQSENLMWDILRNGIISSSKLLSTIKNGP
  TKVFEPAPISTNHYFGGPVAFGLRCEDTVKDIVCKLICGDASANRQFGFMISPTDGIFGVSLDLCVNVES
  QGDFILFTDRSCIYEIKCRFKYLFSKSEFDPIYPSYTALYKRPCKRSFIRFINSIARPTVEYVPDGRLPS
  EGDYLLTQDEAWNLKDVRKRKLGPGHDLVADSLAANRGVESMLYVMTDPSENAGRIGIKDRVPVNIFINP
  RHNYFYQVLLQYKIVGDYVRHSGGGKPGRDCSPRVNIVTAFFRKRSPLDPATCTLGSDLLLDASVEIPVA
  VLVTPVVLPDSVIRKTLSTAAGSWKAYADNTFDTAPWVPSGLFADDESTP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 1410 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009334.1, YP_001129481
• RefSeq ORF: 1410
• Locus ID: 5176208
• MW: 52.7 kDa