DR1 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101440

Myc-DDK-tagged ORF clone of viral ORF for putative DNA-directed RNA polymerase II [Human herpesvirus 6], codon optimized for human cell expression, NP_050176

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DR1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101440 represents NCBI reference of NP_050176 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTCTGACTGCTCGGGCCGGGCACACCTTGCACCGGCTGCCACTGTCCCACTATTGGTGGCTGCTGC
  TTGGGCGCCACAGCTTGCGCCACGTCCACTCTTACTTGCGGTTGCGAAAAGGCCTGCGCCTCCCCCTGCC
  TTGGCCAGAACAGGAGTGCCTCCACCTCCATCCAAAGCCTTACAAGTTTTTGCTGCGATATCCATGCCTC
  ACACGGCAACCTCATCTGCTGCAGGGGTGGCCCGCTGACTCAAGCCTCTGGTTTGATCCTAAGCCCTACC
  ACCCAAGCGCTGACTCTAAGTTGCTTCCCTTGGGACTCATCACGCTGTCAGCCTTCAGCATGAGGGTGAG
  CGAGCCGACGCACTGCAGTGGATTTCATGCTGCAGATCCGTCTCTGAGCTGGCTGACCGGCTCTTCACCT
  TGGCTGGTGCTCCTCCAGGCCCCTGGCGGTAGTCTGTTTTGTCACGATGTGTTCCAGGGAAGGTTGTACC
  TGCTGTCTCACTCCGTCTCACTGTTCCTGAAGACGGGCCTGAGACAGTGCGAGGCGATCTACAGAGCTCC
  CCTGTGGCGGGTGAGACCACTGCCATCCCTGTGGACCTGTCGCGACCCTGATACCGCGTTTCTCCCCAAA
  CTTCTTGCTAGAACTGCGAGAAGGGGCCTTGCAGCCTTCTACGCTCTCTGGCGCCTGCATCTGGGCTCTC
  GGAGCGAGTTGAGTCACCCAGTTCTCGAATGGGAGCGAACGGAACTGGTGCTCACAGACCGCAGGCGCGG
  ATGGCCCTGTACTCATCTTCTGTCCGGCTCCGAGAGTCAAAGAGTGTCTAGCTCCGATGCTGGGGACACC
  TGGAATGCTGCGACCGAAAAAGCTGCCGGCGGGAAAGAAGAGGCTGAGCGGGGAGGTCGGCAGCAAGCAA
  CAGACCGCTTGGCTTCTCCCCATCTTACACGCGGCCGACGGGACTGTGGGAGATCACTGCAGGGTGAGGA
  ACCCTCCGCGGCCGAGGATTTCGCCCGATGTAGGCCACTCCTGGATGAATTGTGTGGGGAGGGGGGTTGG
  CTCCCTTTTGCCTTCTTGACTGCGTCCCCCCATGTGTGCCTGATCCTGACGGAGGGAGGGCCTGTCCTGG
  CCCTGGACCTTAATGATACATCTCTTTGGAGAATCGCCGACGACCTGGAACTTCTCTTGAGGCTGGGCTC
  ACTGTTGCTTTTGTCAGGACTTCGGCTGCCCCTGCGCCCACCATCTGGCTCCGGGGAAGCCGCCAGGAAG
  CCTGGCTACGAAAAGGAAGAGGGAAGAGGTCGCGCTACCACTGCATCAGCCACAGCGGCAACCTCTCCCC
  GGAGACCGACCCGGCCACGGGGCGTGACAGAAAAGGGCCGGGTGACCACAGGGGATGTGCCTTTTAGCGC
  CCACCCTGAATCTGAGGAGCAGACCGACGGTCACCATGGAAGGCAGGAATCTGGCCATGGAGACCAGAGA
  GGTGGGGATGGCCGCGGCCACCGCGATGATGGAGCTCGCAGGCACGCAAATGATGAGACTGAGCCACAGC
  AGAGAGGGGAGCACGAAGACGGTGAACAGACAGACAGTGGGAGGGAGGAGGATGCCCAGGAATCAGAAGT
  GGCACGGCGCGACGAGAAAGGCACAGAGCAGGGAGGGAGTGGACGGAGCTGTGGAAGGGCAACCCAGACA
  TACGGTGGACGAGGCGAGCACGGGGCTTGGTCCAGTATCCCTTTGAGCGTTCCGAGACCTGACCCTCGGG
  TGTGGGTTCCACCACCCCACCTGCTGTTCCCTAGTCCTCTCCCTAGCATTACACCAGTGGAAGATGAACC
  CAGCGCACGACCCCGCTGTCCACCAGGACCGGCTGAAGAACCATCCAAGTGCAGTCCCTGCCCACCTTGC
  CCCTCACCTGATGCCCCACAATCAGCTGTGCCGCGCCTTAGCGCATTGTCTGTGCCTTCCCCCAGCACAG
  CACGGGTTCGCTTCAGTCTCTCTTCTCTGTCTAGCTCATCATCAAGTTCAAGCAGCAGCAGCCCCTCTTA
  CTCTCCCAGTCCACTTAGCCCCCCTTCCCCCGTGTCACCTTCTTCTCCGAGATCCCCCTTTATCTCCCCT
  ATAAGATCACCCGGCCTGCGCGCCAAACCATGGGTTAGTTCCGGTCATCCTGTGGCTTTCCCTCCCGCTC
  CCAGCAGTGCTCCACCCTTCTCTAAGCGGGTCCCCAGCGTGCCAAGCTCTGCCTCTCCCAGCGCTCCGTG
  CATCGGACGGTCCAGACCCCCTAGCGCCCAGACCGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101440 representing NP_050176
  Red=Cloning sites Green=Tags
  
  MPLTARAGHTLHRLPLSHYWWLLLGRHSLRHVHSYLRLRKGLRLPLPWPEQECLHLHPKPYKFLLRYPCL
  TRQPHLLQGWPADSSLWFDPKPYHPSADSKLLPLGLITLSAFSMRVSEPTHCSGFHAADPSLSWLTGSSP
  WLVLLQAPGGSLFCHDVFQGRLYLLSHSVSLFLKTGLRQCEAIYRAPLWRVRPLPSLWTCRDPDTAFLPK
  LLARTARRGLAAFYALWRLHLGSRSELSHPVLEWERTELVLTDRRRGWPCTHLLSGSESQRVSSSDAGDT
  WNAATEKAAGGKEEAERGGRQQATDRLASPHLTRGRRDCGRSLQGEEPSAAEDFARCRPLLDELCGEGGW
  LPFAFLTASPHVCLILTEGGPVLALDLNDTSLWRIADDLELLLRLGSLLLLSGLRLPLRPPSGSGEAARK
  PGYEKEEGRGRATTASATAATSPRRPTRPRGVTEKGRVTTGDVPFSAHPESEEQTDGHHGRQESGHGDQR
  GGDGRGHRDDGARRHANDETEPQQRGEHEDGEQTDSGREEDAQESEVARRDEKGTEQGGSGRSCGRATQT
  YGGRGEHGAWSSIPLSVPRPDPRVWVPPPHLLFPSPLPSITPVEDEPSARPRCPPGPAEEPSKCSPCPPC
  PSPDAPQSAVPRLSALSVPSPSTARVRFSLSSLSSSSSSSSSSSPSYSPSPLSPPSPVSPSSPRSPFISP
  IRSPGLRAKPWVSSGHPVAFPPAPSSAPPFSKRVPSVPSSASPSAPCIGRSRPPSAQTA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 2277 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050176
• RefSeq ORF: 2277
• Locus ID: 1497018
• MW: 82.6 kDa