U42 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101483

Myc-DDK-tagged ORF clone of viral ORF for transactivator [Human herpesvirus 6], codon optimized for human cell expression, NP_050223

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U42
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101483 represents NCBI reference of NP_050223 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATCCAAGGGGCGTGAAACGCAGTCAGCACGATAGGCACAAACAGACTGCATTTAGGACCATTAAGC
  GCTCTATCACCCACCGGCCCACATCTAAGTTTATTAGCCATTTTGCTAAGAACTTCCGGGGGAAGCTGGC
  TCCTCTCAAGCAATTGGATGAAAGTCGGTTGGACGCTCTCAGCCTGACCGAATTGGAACAACTGAAAACA
  ATAATTGAAGAAAAGCAACAGGAGAAGAGAGCACAAAATAACGCCATAACATTTTTGCCTAATCTCCCAA
  CTGTGCCGTTCGCCGACACAAATTTTAGCATCAAATCCCTCGGGCTCAGACCATACAACGGGGACGCACG
  GGACCCTAAACAGCGAATACGGGATCGATTCCCTCAAACGCACGAGCGGATCTGCCTTCTGACAAACGAC
  ATTCTTGAAACCGATCTGCTGCTGCGCTATAGGCAGTGTCTCGACAGTCTCACTAGGGAAGAGAATCAGC
  AGCTGATGGGGGATAGAATTTTCAGTTTGACCAACTCCCCCTGTTTGGCATTCACCGTCGCCACAGTTGA
  AGAAGCCTGTAGTTACTTTAAATTTCATGATCTGCACAACCTTCCAGTTAACCCCCAGGATCTGTTTATG
  TATACTATCACCGTGATGAAATTCGAATTCTTTAATAAGCTGAACATGGCCAAACTTACATGCGTTTTTA
  ATGACAACGGGCATGGTGACATCGAATACCGAAAACTGCGCCAGCTTTGCGGCAAACCAGTGCTCGACAG
  GGAAATGCCTAACTCAGAATTTGAGGTCCAACAGCAAACCCCTGACAGTTTCAGACACCCTATCCAACAA
  GCGATGAGCATCGTGGTGACGTTTGCACGCATACTCAGACAGATAAAGGAACATATTATAAGAACCAAAA
  AGCCTCAGTTTATCCGGGATTTCGACACAGAGCGAGTTGCCGAGAGATATGAATGTGGCCTCATCAGTCG
  CCTCATCGGTAAGCAGTTTAGCAATCACAAGTGCGATGATGTGTCCTGTCAGAATCGGATCGAGAGAATT
  ATGGCGCCCTGGAAACCCAGCCTGTTCTTTTGCACCTATTTTGCCAAGGACGCCCCTAAATTCAAGCTGT
  TCCCCAATTTGCCACACGAGTATAGGAATCTGTCCTTTACCTGCCCTAAAGTGGACATGGAGCCCTCTTG
  CAGTTATAGCACCTCTCGGGACCTGCCCCAGACCAGCCACAGGAGTCATAAGAACCAGGGGACGCCTAAA
  GTGAAATCCAAGGTGTGCGTCGAAAAACCAGACACCAGCAACCTCACGACTACGAAGACCACTACAGAAA
  TTCTCATTGAGGAGTCTATGGAGACAGATAATAAGATTCCAGACCCGCGGGAATTGAACTTCAACCAGGC
  CAAACAAGACGAGATCGTGATTATCAACATCAACGAGAACGTCAACAGCAAACACGAATCAGAAAGTTCC
  GTGGAAATGGATCTCGACCTGGATTACGAAGCCGATACATGCGAGACTAACCTTAACACATACAGCTCCG
  ACAGCGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101483 representing NP_050223
  Red=Cloning sites Green=Tags
  
  MYPRGVKRSQHDRHKQTAFRTIKRSITHRPTSKFISHFAKNFRGKLAPLKQLDESRLDALSLTELEQLKT
  IIEEKQQEKRAQNNAITFLPNLPTVPFADTNFSIKSLGLRPYNGDARDPKQRIRDRFPQTHERICLLTND
  ILETDLLLRYRQCLDSLTREENQQLMGDRIFSLTNSPCLAFTVATVEEACSYFKFHDLHNLPVNPQDLFM
  YTITVMKFEFFNKLNMAKLTCVFNDNGHGDIEYRKLRQLCGKPVLDREMPNSEFEVQQQTPDSFRHPIQQ
  AMSIVVTFARILRQIKEHIIRTKKPQFIRDFDTERVAERYECGLISRLIGKQFSNHKCDDVSCQNRIERI
  MAPWKPSLFFCTYFAKDAPKFKLFPNLPHEYRNLSFTCPKVDMEPSCSYSTSRDLPQTSHRSHKNQGTPK
  VKSKVCVEKPDTSNLTTTKTTTEILIEESMETDNKIPDPRELNFNQAKQDEIVIININENVNSKHESESS
  VEMDLDLDYEADTCETNLNTYSSDSE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 1548 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050223
• RefSeq ORF: 1548
• Locus ID: 1497044
• MW: 60.1 kDa