U74 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101513

Myc-DDK-tagged ORF clone of viral ORF for helicase/primase complex [Human herpesvirus 6], codon optimized for human cell expression, NP_050253

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U74
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101513 represents NCBI reference of NP_050253 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACCTTAGGGGGTGTGCTTGCCACCTGTCTCTCTACTGTGTGTATAACGATTGGGAGAACAAGATCT
  ATCGCGTGCCTATATTTCAGTGTCTGTTCCTCGAGGCCGAAACCCGATCACTGAAGACGTTCCTCATACG
  AGGCCAGTCCCTGGATCAGGAGTCTCTGAATGAAATAGAGGTTACCCGAAAAGAGACCATGCTTTGGGAT
  CTGCAGGAGCAGAGTAATATGATGGATAAGAAGATCGCCGCAATCAGTTCCCTCATTATGAATAACGGAG
  AGCTCCTTAGAAAACTTTCTAAATTCTTTGTTCCATTGACCGTCGTCCTGGGCGACGACGGGTTGGAGAT
  CCTTGAGGCTTATGTGTGCGGGGAGGAACCGATGCTGCCACTTGACACAGTGCCAGTAATCCTGCGGTGC
  GTCGGCGACTATGCCGCCCTCGACACTAAGCATCTGCTCAGTAATGAGTGCACACAGGCATCCAAGAAAC
  TCAGGTTCGGGTATTCTGTGATGGATTTCCACTTCTCCCTTACTGTGTCCGACGTGAAGATATGCTTCTC
  CCACACCGATACAGGTGAAGCAGTTTGCGAGAAAATGAAGCAGATATTCTACTTCTCTGTATGTGCCTTT
  GGTGGAGAACAGGTCCTGCTTGTGACTCCCAAAAACGCCTACGCCCTGCTTTTCGATGACGACCTTTGTT
  TGCTCCTCTTGCAATCCGTTTTTGCCTTCTTGCACGAGAAAATCTTCGCAGTCTACAAGCAGGTCCTGGT
  GCAGCTCTGCGAGTACATCGGACCTGACTTGTGGCCATTCGGTAACGAGCGCAGCGTATCTTTCATAGGT
  TACCCCAATCTCTGGCTGCTGAGCGTGAGTGACCTGGAGAGGCGCGTGCCTGATACCACATACATTTGTA
  GAGAGATCCTGTCATTTTGTGGTCTGGCCCCTATACTGGGTCCTAGAGGAAGGCACGCAATTCCCGTCAT
  CCGGGAACTTTCTGTAGAAATGCCAGGGAGCGAGACCAGTCTGCAGCGCTTCCGGTTCAACAGTCAATAC
  GTATCTTCCGAATCTCTCTGTTTTCAGACTGGTCCCGAAGACACCCACTTGTTCTTCAGCGATTCCGATA
  TGTACGTCGTGACCCTGCCCGATTGCCTTCGCCTGTTGCTTAAGTCCACGGTGCCACGCGCGTTTCTGCC
  GTGCTTCGATGAAAACGCCACGGAAATCGAACTGCTTCTTAAATTCATGTCCAGGCTGCAGCATAGGAGC
  TACGCTCTTTTTGACGCCGTCATCTTCATGCTCGACGCATTTGTGAGCGCCTTTCAGCGGGCATGCACAC
  TCATGGAGATGCGCTGGCTCTTGGTCAGAGACCTGCACGTTTTCTACCTGACATGTGACGGAAAGGATAG
  CCACGTTGTGATGCCCCTGCTGCAGACCGCCGTGGAAAATTGTTGGGAAAAGATCACCGAAATAAAACAG
  AGGCCTGCCTTTCAATGTATGGAAATTTCACGGTGCGGGTTTGTGTTCTACGCTAGATTCTTTTTGAGCT
  CCGGGCTCTCCCAGTCAAAGGAAGCTCACTGGACAGTGACTGCGAGTAAATATTTGTCCGCCTGCATCAG
  GGCTAATAAGACCGGTTTGTGCTTCGCCTCCATCACTGTGTACTTTCAGGATATGATGTGCGTATTCATA
  GCAAATAGGTATAATGTCTCTTATTGGATCGAGGAGTTTGATCCAAACGACTACTGTCTGGAGTATCACG
  AGGGCCTCCTGGATTGTAGTCGATACACCGCCGTCATGAGTGAAGACGGTCAGCTGGTGCGGCAGGCCAG
  AGGCATAGCCCTCACTGATAAAATTAACTTTTCCTACTACATATTGGTCACCCTGAGGGTTCTTCGCCGC
  TGGGTGGAGAGTAAGTTCGAAGATGTGGAGCAAGCTGAGTTTATCAGGTGGGAGAATCGCATGCTCTATG
  AACACATTCACCTGCTGCATCTCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101513 representing NP_050253
  Red=Cloning sites Green=Tags
  
  MHLRGCACHLSLYCVYNDWENKIYRVPIFQCLFLEAETRSLKTFLIRGQSLDQESLNEIEVTRKETMLWD
  LQEQSNMMDKKIAAISSLIMNNGELLRKLSKFFVPLTVVLGDDGLEILEAYVCGEEPMLPLDTVPVILRC
  VGDYAALDTKHLLSNECTQASKKLRFGYSVMDFHFSLTVSDVKICFSHTDTGEAVCEKMKQIFYFSVCAF
  GGEQVLLVTPKNAYALLFDDDLCLLLLQSVFAFLHEKIFAVYKQVLVQLCEYIGPDLWPFGNERSVSFIG
  YPNLWLLSVSDLERRVPDTTYICREILSFCGLAPILGPRGRHAIPVIRELSVEMPGSETSLQRFRFNSQY
  VSSESLCFQTGPEDTHLFFSDSDMYVVTLPDCLRLLLKSTVPRAFLPCFDENATEIELLLKFMSRLQHRS
  YALFDAVIFMLDAFVSAFQRACTLMEMRWLLVRDLHVFYLTCDGKDSHVVMPLLQTAVENCWEKITEIKQ
  RPAFQCMEISRCGFVFYARFFLSSGLSQSKEAHWTVTASKYLSACIRANKTGLCFASITVYFQDMMCVFI
  ANRYNVSYWIEEFDPNDYCLEYHEGLLDCSRYTAVMSEDGQLVRQARGIALTDKINFSYYILVTLRVLRR
  WVESKFEDVEQAEFIRWENRMLYEHIHLLHLN
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 1986 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050253
• RefSeq ORF: 1986
• Locus ID: 1497074
• MW: 76.5 kDa