U55 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101496

Myc-DDK-tagged ORF clone of viral ORF for hypothetical protein HhV6Bgp062 [Human herpesvirus 6], codon optimized for human cell expression, NP_050236

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U55
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101496 represents NCBI reference of NP_050236 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCTTCAAGAACACACTGGCTTATTATAATTTCTCCATAACCTACACTCAGGATTATAAAAACACAC
  CAAGGCTCAATACTAGAGTTCTCCATCCCTCTTGCAAGATCACATCATCCTTGAAGAAGACGCAGTACCT
  TAAGTCTAAAGTGAAGCATAAAATGTTTTTTTGCACAAACATGTACAGCCCTCAGCTGAACCATGTACTG
  CAACTTGTGTTCGATTGCAACCACATCGTGCCGAATAGCTACCAGGAGATAACACTGAAATGTCGGATCA
  GCTCTACTAAACCAGGCATCCTCTTCATCACCGACGGAACCGAGTCCGAGACAGAAGATGTGCTCTTCGA
  TGTGCAGTTCCTCGAAAATACCATGACTATTAAAATCAAAATATTGCCCATCTCAAGAACCGTGCCGGTA
  CGCATCCACCTGTTCTCTATCCCCATTTACCTTCCCAGCCCGGCCCTGGAAATCGACGACATTAATGCAC
  TCTGCCAAAAGGAAAATATAGATCCAATTGTCTACGAGCAGTACGGTCTTACCAATATGCCAATCTCTAC
  TACCAATGTCATTAACCAGTTTAAGGTGGTGTCTAACGACAGTGTAGAGACTAGTCTGATCCTGTTGAAC
  CTCTCTTGGACAGAGTCACCCTACCAGTCACAAAAGCTGGATCGGTACGGGCGGAAGTTGTTGATCGTCT
  TCTCCGTGTTTAGTATAGACACCAGGAAATGTTCATACTGGAAAAACCTCTTTAAAAGCATGATTCCTCT
  TTGCCGGGTGAACATGATCTCCGAGCCCGGCATCTCTATCTACAAGATCGAGTTCAACGACCCCTTCTTG
  ACCATCTTTCTGCGCAACGTAGTACTCCCTGAGAATGAAAGAAGATTCCCCATTACCAAAGAGTGCTATC
  TCCGATTGACATTTTCACCACCAAATCGCAGTACAGTTACCCTGAATATGAACATGCCTTACTTCAAGAT
  CTGCGCCGATGGAAAGTCACTGGAGGTGTTTTTCCCCAGCGATATGTCACTCGTGATCAACACAAACAAG
  GAAATTACGCTGCGAGGAAAGTTTACAAACAAGAAGATCGTCGGGATATTTATTCCCGTACAGACCCATG
  TCTTCAAGCTGTATCCATTTCTCTGGTTCGCACAAGAACCACTGAAACTCCGCATCTCCTGCGAGAAGAC
  CGTCTCAGTCACCGAACATGACATGCTGGGCAGAATCTTTTTTGTCACAAAGAAGCTTTTCAGAAAGCCA
  TTCCGAAGCCTCTCCAATAGCGACATGAAGTCACTGCTTACGCATACTGAGAACACGGACAAACCATTTG
  AGGTGCACTTTATGGGTAATTGTTTCCCAGCCTATCTGCTCCCAGAACTGACTCTCCACCCACTGGAGTA
  TGAGGACAAGCCCGCACGGGGGAACCCCCAAATCCTCCCGTCCGTGGAGAAGCATAGCATTATCAAAATG
  AGAATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101496 representing NP_050236
  Red=Cloning sites Green=Tags
  
  MLFKNTLAYYNFSITYTQDYKNTPRLNTRVLHPSCKITSSLKKTQYLKSKVKHKMFFCTNMYSPQLNHVL
  QLVFDCNHIVPNSYQEITLKCRISSTKPGILFITDGTESETEDVLFDVQFLENTMTIKIKILPISRTVPV
  RIHLFSIPIYLPSPALEIDDINALCQKENIDPIVYEQYGLTNMPISTTNVINQFKVVSNDSVETSLILLN
  LSWTESPYQSQKLDRYGRKLLIVFSVFSIDTRKCSYWKNLFKSMIPLCRVNMISEPGISIYKIEFNDPFL
  TIFLRNVVLPENERRFPITKECYLRLTFSPPNRSTVTLNMNMPYFKICADGKSLEVFFPSDMSLVINTNK
  EITLRGKFTNKKIVGIFIPVQTHVFKLYPFLWFAQEPLKLRISCEKTVSVTEHDMLGRIFFVTKKLFRKP
  FRSLSNSDMKSLLTHTENTDKPFEVHFMGNCFPAYLLPELTLHPLEYEDKPARGNPQILPSVEKHSIIKM
  RI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 1476 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050236
• RefSeq ORF: 1476
• Locus ID: 1497057
• MW: 57.2 kDa