DR6 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101441

Myc-DDK-tagged ORF clone of viral ORF for transactivator [Human herpesvirus 6], codon optimized for human cell expression, NP_050180

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DR6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101441 represents NCBI reference of NP_050180 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTACACGACACACCCAGACCCGAGACGGCAGGATCGCTATTCGCCGCGACGGCGCCAGGCTTGCAC
  ATGCTAGGGCGAGAGCCAGATTCGAATGGCTGCTGCTGGCCCGGGGTAGGCCCTCCAAACTCTACGGCTA
  CACCTCCAGACACCGGGGAGAACGAATTCACCTCCCTTGGCCCAGATATTGGTGTCTGGAGCTGCACCCC
  GATCCCTACCGCGACGCCCGGTCCGCCACAGTGTGGGGGCACCGCTGGGGTTGGCCACCCACTCATGTTA
  GGCCCAGATCAGTGCAGGATTGTGCCCTGGACAGCAGTTTGTACGTGTGCTGCGGCTACGGCGAGAAACT
  GCAGCCTGTGGGTTTCGTCAGCTCCTACCTGACCCACAGTCCACTGGACACTCTCAGAGTGCTCCTGGTA
  GGAAGGGATGGAGCGGTCTATGTGCACCACATGCGCGCTGCAAGACTGTGCAGATTGGCTTCCAACGTGA
  CCGAGTTTGCGAGACGAGGTCTTCAGCGGGACCCAGTTGCCTATGAAGAGGACTTGGAGCTGCCCGATCG
  GAGGATGTGTGGCACCAATGTAAGGCACCTCTTCGACGTTATCGCTGCCGCTGCAGACGAACACGACCTT
  CTTACGGTTGGAGGACTCTGCCAGACCCATGCCGGCGTGAGCTGCGAGCTTCTCGAGACAGTAAGGGATC
  CTTGGACCGCCGTGCCCGGGGTGCGGATGACGCTCACAGTGGCTCGGGCTCAGTATCGCCTCTGGCCCGA
  TGCCCGCCGACAGTTGAGACTGCATCTTTACGCGGGGCATCCCCTGGGTCCTTGGATTGTGTGCGCCGTC
  CTTAGTCGAGAGCGCGAGACTCAGACGCCCAGCCCGCCAATCGGATCCGGGGGTGTGATCTTGGGAAACG
  TCCCAACCCCCGGCCCCCGCGAGGTGGAAACTGCCTGGGTTATCGTGACCGTCGCCGGCCCGCTTCTCAG
  TTTCTGGCCAGACAATGGAAAGATATGCCGATTGGCCAACTCTTTCGCCGCTCTGTGGCGGATGGGACCC
  CGGGCCATGAGGGGGCATTGGACTTACAGTGCACCAGGGCGCCACCTTCCAGGGGACGCTTGGCCTCTTT
  GTGAGCACGTGCGACCTCCCGTTGGCAAACTGCCAAGGCAGAGAGCCTACCTCGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101441 representing NP_050180
  Red=Cloning sites Green=Tags
  
  MTTRHTQTRDGRIAIRRDGARLAHARARARFEWLLLARGRPSKLYGYTSRHRGERIHLPWPRYWCLELHP
  DPYRDARSATVWGHRWGWPPTHVRPRSVQDCALDSSLYVCCGYGEKLQPVGFVSSYLTHSPLDTLRVLLV
  GRDGAVYVHHMRAARLCRLASNVTEFARRGLQRDPVAYEEDLELPDRRMCGTNVRHLFDVIAAAADEHDL
  LTVGGLCQTHAGVSCELLETVRDPWTAVPGVRMTLTVARAQYRLWPDARRQLRLHLYAGHPLGPWIVCAV
  LSRERETQTPSPPIGSGGVILGNVPTPGPREVETAWVIVTVAGPLLSFWPDNGKICRLANSFAALWRMGP
  RAMRGHWTYSAPGRHLPGDAWPLCEHVRPPVGKLPRQRAYLD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 1176 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000898.1, NP_050180
• RefSeq ORF: 1176
• Locus ID: 1497019
• MW: 44.3 kDa